Effectiveness Of Stepping Dose Ozonated Virgin Coconut Oil For Wound Healing In Autologous Full Thickness Skin Graft In Terms Of Fibroblast Proliferation And The Expression Of Vascular Endothelial Growth Factor In Sprague Dawley Rats

Abstract

Abstract \nBackground: Now, Skin grafts are one of the therapies of choice in the wound healing process. And this is still developing today. The process of formation fibroblas and the formation of vascular endothelial growth factor have an important role in healing skin graft wounds. Ozonated virgin coconut oil has an important role in wound healing. \nObjective: To prove the effect of ozonated virgin coconut oil in various doses in increasing number of fibroblas and expression of vascular endothelial growth factor of skin graft wounds. \nMethods: This study is an experimental study with Randomized parallel study with controlled group design on 40 Sprague Dawley rats which are performed an autologous skin graft at the same time. Samples were divided randomly into 8 groups (K1 and K2 = without ozonated VCO), (A1 and A2 = ozonated VCO 50,4 mg/ml), (B1 and B2 = ozonated VCO \xa0103,2 mg/ml), (C1 and C2\xa0 = ozonated VCO 204 \xa0mg/ml). Evaluation of the amount of fibroblas was done by staining hematoxylin & eosin after the 6th day and the expression of vascular endothelial growth factor \xa0was done by staining immunohistochemistry after the 12th \xa0day after skin graft. \nResults: Statistical analysis of the amount of fibroblas after the 6th\xa0 day was found to be significantly different between the K1 vs A1 group (p = 0.029), K1 vs B1 (p = 0.004), K1 vs C1 (p = 0.000), on the 12th day was found significantly different the amount of fibroblast between K2 vs A2 ( p = 0,029 ), K2 vs B2 ( p = 0,010 ), K2 vs C2 ( p = 0,001 ). The expression of vascular endothelial growth factor on the 6th day was found a significant difference between the K1 vs A1 group (p = 0.024), K1 vs B1 (p = 0.005), K1 vs C1 (p = 0.001), on the 12th day was found significantly different the expression of vascular endothelial growth factor between K2 vs A2 ( p = 0,011 ), K2 vs B2 ( p = 0,036 ), K2 vs C2 ( p = 0,004 ) . \nConclusion: Ozonated VCO can increase the amount of fibroblas and expression of vascular endothelial growth factor \xa0in the wound healing process of autologous skin graft in Sprague Dawley rats. \nKeywords: Ozonated VCO, full thickness skin graft, fibroblas, VEGF.