Quantification of anti-quorum sensing and antibiofilm activity of Phyllosphere bacteria against food spoilage bacteria

Abstract

Food spoilage and microbial contamination require\xa0 attention during the food production process since the presence of these bacteria can create problems including the formation of biofilms produced by these\xa0 bacteria. Biofilm formations are initiated through cell-to-cell communication which is called quorum sensing mechanism. Hence, inhibition of this communication\xa0 mechanism could be one of the solutions to inhibit\xa0 biofilm formation. Therefore, exploration of bioactive compounds from various sources including\xa0 hyllosphere bacteria with anti-quorum sensing inhibition activities is important. Phyllosphere bacteria are a community of bacteria found on the surface of plant leaves at a very\xa0 large population. These bacteria can produce bioactive compounds that can inhibit quorum sensing mechanism. In this study, 54 phyllosphere bacteria\xa0 isolates were tested, 8 bacterial isolates had potential effect to inhibit quorum sensing. From biofilm inhibition assay, the highest percentages were showed by\xa0 ifferent phyllosphere isolates against each pathogen. Whereas, for biofilm destruction assay, JB 8F isolate had the highest percentage of destruction biofilm activity\xa0 against biofilm formed by Bacillus cereus and\xa0 Shewanella putrefaciens. Eight isolates of phyllosphere\xa0 bacteria had the potential as quorum quencher and\xa0 anti-biofilm agents, both for inhibition and destruction of biofilm.\n\xa0