Physicochemical Properties and Determination of Some Bioactive Phytochemical Constituents From Linum Usitatissimum L Oil (Flaxseed) by Gas Chromatography-mass Spectrometry (GC-MS) and Atomic Absorption

Abstract

Flaxseed (Linum usitatissimum L.) is an important source of oil, The dried seed of flaxseed 100 g, produced 38% of oil extracted by n-hexane, the physiochemical properties of flaxseed such as acid value (0.71 mg KOH/g) as oleic acid, peroxide value (2.69 meq O2/kg), saponification value (186.50 mg KOH/g oil), and iodine value (96.67 g/100 g), density (0.91 g/cm3), refractive index (1.39 and at 28°C), viscosity (CST) (68.33). Gas Chromatography-Mass Spectrometry (GC-MS) was used to identify bioactive phytochemical constituents it contained 26 compounds identified, the major compound were 9-Octadecenoic acid (Z)-, methyl ester (Oleic acid) (43.72) act as antioxidants, anti-cancer, Hexadecanoic acid, methyl ester (Palmitic acid) (17.36) act as Anti-oxidant, decrease blood cholesterol, anti-inflammatory, 9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z) fatty acids:α-Linolenic acid, an omega-3 (n-3) fatty acid γ-Linolenic acid, an omega-6 (n-6) fatty acid (15.72), Octadecanoic acid Methyl stearate (13.13) used As a food additive, soaps, cosmetics, detergents lubricants, softening and release agents,13-Docosenoic acid, methyl ester, (Z)-(1.31), cis-11-Eicosenoic acid, methyl ester (0.96) act as Antibacterial, antitumor, antifungal, cytotoxic. Atomic absorption is used to identify minerals content it contains Calcium 1180ppm, Copper 4.1ppm, Ferric 15.7ppm, Zinc 34.58ppm.