Expressions and significances of miR-135a-5p and TXNIP in papillary thyroid cancer

Abstract

Objective \nTo detect the expression of miR-135a-5p and thioredoxin interaction protein (TXNIP) in papillary thyroid carcinoma (PTC) and to analyze their clinical significances. \n \n \nMethods \n48 patients with PTC who underwent operation and confirmed by pathological examination in Shandong Provincial Third Hospital from March 2017 to March 2018 were selected. Tumor tissues and adjacent tissues were collected. The expression of TXNIP protein was detected by streptavidin peroxidase (SP) and Western blot, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-135a-5p. \n \n \nResults \nThe positive expression rate of TXNIP protein in adjacent tissues of PTC patients was significantly higher than that in tumor tissues (P<0.05); the expression level of TXNIP protein in tumor tissues of PTC patients was significantly lower than that in adjacent tissues (P<0.05); the expression of miR-135a-5p in PTC patients was significantly lower than that in adjacent tissues(P<0.05); in patients with PTC tumor diameter (≥2 cm) and lymph node metastasis, the proportion of patients with low expression of miR-135a-5p was significantly higher than those with high expression of miR-135a-5p(P<0.05); in patients with PTC tumor diameter (≥2 cm) and lymph node metastasis, the proportion of patients with negative TXNIP expression was significantly higher than that of patients with positive TXNIP expression (P<0.05); there was a significant positive correlation between the expression of miR-135a-5p and TXNIP in PTC patients (P<0.05). \n \n \nConclusions \nThe expression levels of miR-135a-5p and TXNIP in tumor tissues of PTC patients are lower than those in adjacent tissues. The expression levels of miR-135a-5p and TXNIP were positively correlated with the increase of tumor diameter and lymph node metastasis. The low expression of miR-135a-5p and TXNIP may be related to the occurrence and deterioration of PTC tumors. \n \n \nKey words: \nMicroRNAs;\xa0Carrier proteins;\xa0Papilloma;\xa0Thyroid neoplasms