Archive | 2019
Molecular analysis of hemaglutinin and neuraminidase genes of avian influenza A (H5N6) virus in Shandong province from 2015 to 2017
Abstract
Objective \nTo analyze the sequences of hemaglutinin (HA) and neuramindase (NA) genes in four strains of avian influenza A (H5N6) virus isolated from environment during the period of 2015-2017, so as to explore genetic variations and molecular biological characteristics of the virus. \n \n \nMethods \nVirus isolation and gene sequencing were performed for avian influenza virus strains detected as H5 subtype. The genetic evolution and characteristics of molecular variation were analyzed by MEGA 6.0. \n \n \nResults \nThe similarities of HA and NA genes in four avian influenza A (H5N6) virus strains isolated in Shandong province were 94.6%-99.4% and 95.4%-98.6%, respectively. The phylogenetic analyses indicated that the HA genes were grouped into 2 separated clades of the 2.3.4.4 cluster. The amino acid sequences analysis revealed multi-locus mutations both in HA and NA. Glycosylation analysis showed that there were 4-8 sites of N-glycosylation. \n \n \nConclusions \nThe avian influenza A (H5N6) virus strains showed molecular characters of high pathogenicity, but no sign of receptor-binding to facilitate the infections of human. \n \n \nKey words: \nAvian influenza A(H5N6) virus;\xa0Hemaglutinin;\xa0Neuraminidase;\xa0Molecular characteristics