Effects of catalpol on oxidative stress and cardiac function in type 2 diabetic rats

Abstract

Objective \nTo study the protective effects of catalpol on cardiomyocytes in type 2 diabetic rats. \n \n \nMethods \nBased on the method of random number table, 8-wee k-old SD rats were randomly divided into normal group (10 rats) and experimental group (90 rats). The rats in the experimental group that were fed with high-fat and high-sugar diet for 8 weeks were injected intraperitoneally with STZ (15 mg/kg), with fasting blood glucose≥16.7mmol/L as a model for type 2 diabetes. Then based on fasting blood glucose and body weight, the rats meeting the criteria of type 2 diabete mellitus were randomy divided into five group, namely, the model group, the control group, the low or medium and high doses of the catalpol group, and there were 12 rats in each group. The normal group and the model group were filled with distilled water (5 ml/kg•d, the control group were treated with metformin (90 mg/kg•d), and Low, medium and high dose group were intragastric administration of Catalpol(2.5, 5, 10 mg/kg•d). Then, the level of glucose, myocardial oxidative stress related protein, cardiac strcture and function change were measured after 12 weeks of intervention in rats. \n \n \nResults \nCompared with the model group, the level of fasting blood glucose in catalpol low, medium and high dose groups was significantly lower (P<0.05). The levels of SOD (197.43 ± 8.85 U/ml, 186.54 ± 5.89 U/ml, 175.62 ± 7.67 U/ml vs. 157.75 ± 11.29 U/ml) in myocardial tissue of those groups significantly increased (P<0.05), the levels of MDA (7.26 ± 0.72 nmol/mg, 8.58 ± 0.93 nmol/mg, 10.62 ± 0.59 nmol/mg vs. 14.80 ± 0.71 nmol/mg) in myocardial tissue of those groups significantly decreased (P<0.05), the E/A value (1.25 ± 0.18, 1.09 ± 0.14, 0.97 ± 0.11 vs. 0.51 ± 0.11) in those groups significantly increased (P<0.05), the E/E values (12.33 ± 0.73, 13.26 ± 1.07, 14.73 ± 1.23 vs. 20.54 ± 1.64) and the IVRT (21.90 ± 2.60 ms, 22.05 ± 2.84 ms, 24.42 ± 2.22 ms vs. 27.40 ± 2.81 ms) in those groups significantly decreased (P<0.05). \n \n \nConclusions \nThe catalpol can reduce oxidative stress, improve cardiac function and protect cardiomyocytes. \n \n \nKey words: \nCatalpol;\xa0Diabetes mellitus, type 2;\xa0Oxidative stress;\xa0Atrial function;\xa0Rats