International Journal of Anesthesiology and Resuscitation | 2019
Protective effects of hypoxia pretreatment on mitochondrial respiration function in oxygen-glucose deprivation human brain microvascular endothelial cell
Abstract
Objective \nThe objective of this research is to investigate the protective effects of hypoxia pretreatment on oxygen-glucose deprivation human brain microvascular endothelial cell (HBMVEC) via detecting mitochondrial function and hypoxia-inducible factor-1α(HIF-1α) and succinate dehydrogenase complex iron sulfur subunit B(SDHB) expression in oxygen-glucose deprivation HBMVEC. \n \n \nMethods \nCultured HBMVEC were randomly divided into 5 groups: sham group (group A), oxygen-glucose deprivation group (group B), oxygen-glucose deprivation+hypoxia pretreatment group (group C), oxygen-glucose deprivation+hypoxia pretreatment+dimethyloxalylglycine (DMOG)(group D) and oxygen-glucose deprivation+hypoxia pretreatment+2-ME (group E). Then, cell apoptosis was detected by Annexin V kit. ATP content was analyzed by ATP assay kit. The level of HIF-1α and SDHB expression were determined by Western blot. \n \n \nResults \nCompared with group A, the expression of HIF-1α was up-regulated, ATP content was significantly decreased and cell apoptosis was increased in group B, group C, group D and group E (P<0.05), the expression of SDHB was significantly down-regulated in group B, group C and group E (P<0.05). Compared with group B, the expression of HIF-1α and SDHB was up-regulated, ATP content was significantly increased and cell apoptosis was decreased in group C and group D(P<0.05). \n \n \nConclusions \nHypoxia pretreatment can protect mitochondrial respiration function in oxygen-glucose deprivation HBMVEC via promoting HIF-1α and SDHB expression. \n \n \nKey words: \nOxygen-glucose deprivation;\xa0Human brain microvascular endothelial cell;\xa0Hypoxia pretreatment;\xa0Hypoxia-inducible factor-1α;\xa0Succinate dehydrogenase complex iron sulfur subunit B