Modified water sac method and intraperitoneal perfusion for establishment of liquid animal model with high intra-abdominal pressure: a comparative study

Abstract

Objective \nTo investigate the advantages and disadvantages of modified water sac method and intraperitoneal perfusion for establishment of liquid animal model with high intra-abdominal pressure. \n \n \nMethods \nTwenty healthy adult New Zealand rabbits were randomly divided into two groups (n=10 each) . Group I was given intraperitoneal perfusion to establish liquid animal models with high intra-abdominal pressure. Group Ⅱ was treated with water sac method to establish modified liquid animal models with high intra-abdominal pressure. The infusion volume and the corresponding abdominal pressure were measured, and the pressure-capacity curve was drawn. When the intra-abdominal pressure was 10, 20, 30, 40, and 50 cmH2O (1 cmH2O=0.098 kPa) , the infusion volume of normal saline was recorded. \n \n \nResults \nThe pressure-volume curve of the animal models with high intra-abdominal pressure showed a positive correlation between abdominal pressure and infusion volume in the two groups of animal models. Group I (perfusion model) regression equation: y=-68.84+0.0762x (R2=0.9068, P=0.0001) . Group Ⅱ (water sac model) regression equation: y=-132+0.1643x (R2=0.8023, P=0.0064) . When the intra-abdominal pressure was 10, 20, 30, 40 and 50 cm H2O, the infusion volume of water sac method was significantly less than that of perfusion method[ (920±35.28) ml vs (1 070±60.54) ml, (970±40.44) ml vs (1 260±64.62) ml, (1 000±41.36) ml vs (1 370±65.28) ml, (1 025±44.22) ml vs (1 470±68.68) ml, (1 045±48.60) ml vs (1 520±75.76) ml], all P<0.01. \n \n \nConclusion \nThe animal model with high intra-abdominal pressure established by modified water sac method is a new animal model better than that established by intraperitoneal perfusion. It justifies use for reference in establishing animal models of high intra-abdominal pressure in clinical research. \n \n \nKey words: \nIntra-abdominal hypertension;\xa0Animal model;\xa0Water sac;\xa0Perfusion