Is There an Association Between Extreme Levels of Boron Exposure and Decrease in Y:X Sperm Ratio in Men? Results of an Epidemiological Study

Abstract

Objectives: A negative association between Y:X sperm ratio and high levels of boron exposure was suggested in an epidemiological study conducted in boron mining areas of China. That study, however, was criticized by many authors due to some weaknesses in the study design. The present epidemiological study was designed to corroborate or refute the above-mentioned negative association between boron exposure and Y:X sperm ratio in men. Materials and Methods: The study was conducted in a boric acid production zone in Bandırma. One hundred sixty-three male workers voluntarily participated in our study. The workers employed in the boric acid production facilities were assigned as the exposed workers (n=86). The control group was composed of workers employed in the steam power plant, energy supply unit, demineralized water plant, mechanical workshop, etc. (n=77). Blood and semen samples were sampled from the participating workers at the end of the work shift. Y:X sperm ratio in semen samples was determined by fluorescence in situ hybridization. Boron concentrations in semen and blood samples were determined using inductively coupled plasma-mass spectrometry. Results: Boron-mediated adverse effect on the Y:X sperm ratio was not determined in workers in our study even under extreme occupational exposure conditions. The results of our study refute the negative association between Y:X sperm ratio and high levels of boron exposure that was suggested in a previously published epidemiological study conducted in boron mining areas of China. Conclusion: The results of our study indicate that boron-mediated adverse effects on the Y:X sperm ratio do not seem possible even under occupational boron exposure conditions.