Multiplicação in vitro de Xylopia aromatica em diferentes meios de cultura e concentrações de BAP

Abstract

The purpose of this study was to develop an in vitro multiplication methodology for Xylopia aromatica , aiming the production of seedlings, evaluating culture media and concentrations of more suitable growth regulators. Nodal segments from seedlings produced from seeds and kept in a nursery were used as explants. The Murashige & Skoog (MS) and Wood Plant Medium (WPM) culture media were tested supplemented with 800 mg L -1 of polyvinylpyrrolidone (PVP), 30 g L -1 of sucrose, 0.054 µM of naphthaleneacetic acid (NAA), 6 g L -1 \xa0of agar and concentrations of 2.22 and 3.55 µM of benzylaminopurine (BAP) for in vitro multiplication. At 30 days, the number of lateral sprouts emitted and percentage of explants forming callus at the base were evaluated. The highest multiplication rates were obtained on MS medium supplemented with 3.55 µM of BAP. Regarding callus formation, the MS medium promoted greater formation than WPM medium, however it did not compromise the formation of shoots. It was possible to establish the introduction and multiplication phases, thus defining the culture medium and concentrations of growth regulators most suitable for in vitro culture of Xylopia aromatica .