Evaluation the Histological Effects on Brian and Skeletal Malformations in Fetuses and Neonates of Rats Treated with the Antibiotic Levofloxacin

Abstract

This study desig to reveal the possible negative effects of the antibiotic Levoflexacin (Levo) on the fetuse numbers (total, living, absorbed and dead ) during three periods of pregnancy (20,16,7) days respectively and on some histological effects of brain of fetuses during two periods of pregnancy (20,16) days respectively and the skeletal malformations in fetuses for pregnancy period(20) days and neonates after birth ,the study was carried out in the animal house of the Department of biology at the College of Education for Girls University of Kufa, This study lasted from November -2020 to March2021, 60 adult female rats and 60 fertile male rats Rattus ratuss were used in the current study, female rats were with an average age of 12 weeks and an average weight of 240 g, while male rats were at an average age of 11 weeks and the average weight of 230 g which was used for the purpose of mating with females only, and after mating between males and females rats and obtaining a sufficient number of pregnant females rats, the pregnant rats were divided into 4 groups, each containing 20 pregnant female rats, The first group was treated as a control group that was dosed with physiological salt only, the second group was dosed with the antibiotic levofloxacin at a concentration of 500 gm / kg of body weight and the third group was dosed with the antibiotic levofloxacin at a concentration of 750 gm / kg of body weight, the first 15 pregnant female rats from all groups were dissected during the two pregnancy periods (7,16, 20) days respectively , It included each group 5 pregnant females for each pregnancy period, while the remaining 5 pregnant females from each group were left to the birth, all pregnant animals were dosed from the first day of pregnancy by a stomach tube with single dose per day. The results of the present indicated that there was a significant decrease(P<0.05) in the numbers of total and the numbers of live embryos, while the numbers of absorbed and dead embryos were significantly increased (P<0.05) in groups of animals treated with the antibiotic levofloxacin at concentrations 500 g/kg and 750 g/kg of body weight for pregnancy periods (7,16,20) days respectively compared with the numbers of all embryos and for the three pregnancy periods (7,16,20) days respectively in the control groups, and the results pointed to significant differences in two groups treated with concentrations of (500,750) g/kg of body weight during the three pregnancy periods when comparing them. The results of the study showed that the brain sections of the embryos in the pregnant rats groups in the control groups during the two pregnancy periods (16,20) days respectively did not show any pathological changes in their