Polymorphism in Toll-like Receptor 10 and Tuberculosis Susceptibility in Egyptian Population

Abstract

Background: One-third of the world’s population is infected with Mycobacterium\ntuberculosis. Difference in clinical outcome of infection implies that host genetics may be\nimplicated in such variability. Investigations of Toll-like receptors (TLRs) revealed new\ninformation regarding the immunopathogenesis of tuberculosis. Toll-like receptor 2\n(TLR2) mediates crucial immune response against Mycobacterium tuberculosis. There is\nargument that Toll-like receptor (TLR10) participate in tuberculosis susceptibility by\nacting as a signaling modulator for TLR2. Objectives: The aim of this study was\ninvestigating the relationship between TLR 10 SNP 720A/C (rs11096957) and increase\nsusceptibility to tuberculosis. Methodology: Eighty patients with radiological,\nmicrobiological and clinical proven active pulmonary tuberculosis (T.B) were included\nin this study. (TLR10) polymorphisms and allele distributions were compared between\nthese 80 patients and 70 healthy control subjects. Peripheral blood samples were taken\nfrom all patients and controls. Genotyping was accomplished by polymerase chain\nreaction-restriction fragment length polymorphism (PCR-RFLP). Results: When we\ncompare T.B cases with controls, a statistically significant association was observed\nbetween T.B susceptibility and SNP 720A/C (rs11096957) in (TLR10). Allele (A) was\nmore frequent in tuberculous cases while allele (C) was more common in controls. It was\nreported that the AA genotype of (TLR10) SNP rs11096957 was considerably related to\nthe increased risk of developing pulmonary T.B. Homozygosity (AA) has been associated\nwith predisposition to disease by comparing cases to controls (P = 0.045; OR = 2.0;\n95% C.I. = 1.0- 4.0). A/C heterozygosity was considerably different in tuberculous cases\nthan in healthy controls with lower risk of developing tuberculosis (P = 0.044; OR =\n0.5; 95% C.I. = 0.26 –0.98). Conclusion: TLR10 SNP rs11096957 polymorphism is a\nrisk factor for tuberculosis infection.