“Some Anti-viral Activities in Spring Food Material, Mugwort”

Abstract

Background A corona pandemic has been spread all over the world emerging some mutants sub-strains. Many trials were reported including anti-viral agent and vaccines in many developing countries. However, none of the system still remained to be absolute for concur this evil corona virus. Therefore, we tried to review the traditional method for impel the way for this microorganism, especially suggesting traditional food additives. Coronavirus 2 (SARS-CoV-2) and has now spread to more than 200 countries and territories, infecting March in US peoples 450,000 peoples deaths US wide. The mugwort had been traditionally used as food additive as well as famous health care material, such in muxibution can reported as traditional health care in Eastern Asian countries. Our in vitro experiment also by the human cytomegalovirus (HCMV) of the DNA virus were suppressed for replication by this material. Introduction Mugwort (Artemisia indica Willd. var. maximowiczii) had been traditionally included in daily life of Japan. For mugwort had been succeeded to employ as medicinal plant in East Asian countries as food additive as well as health care substance, including heating therapy such as in moxybution. In Japan special rice cake that turn to βto α-polysaccharide had been prepared artificially in early spring in order to featuring newly grown mugwort for traditional foods. For the people had been confirmed and including their annual life for health maintenance especially for infectious disease by the microorganism. Materials and Methods Human embryonic pulmonary fibroblast (HEL), obtained from human fetal lungs cultivated in 9.6 cm2 shell until confluence with Dulbeccos Modified Eagle Medium (DMEM) medium was carried out, supplemented with 8% amount of fetal bovine serum (FCS). Towne strain of HCMV has been adsorbed and infected in a variety of infections (MOI) = 1 in HEL cells. After incubation at 37 °C for 1 hour, concentrations of 1, 3, 5% of mugwort was added and cultivated for 6 to 12 days, and anti-HCMV effects were quantified by the cytopathic effect under the International Journal of Medical Science and Health Research Vol. 5, No. 03; 2021