Genetic diversity and drug resistance of Mycobacterium tuberculosis in Ecuador.

Abstract

BACKGROUND\nThe genetic diversity of Mycobacterium tuberculosis in Quito, Ecuador is not well known.\n\n\nOBJECTIVE\nTo investigate mutations related to drug resistance and bacterial genotypes in M. tuberculosis strains in Ecuador.\n\n\nDESIGN\nThis was a retrospective study of M. tuberculosis isolates from 104 patients. Isolates were phenotypically resistant to rifampicin (RMP) and/or isoniazid (INH). The genotype was determined using 24-locus mycobacterial interspersed repetitive units-variable-number tandem repeats (MIRU-VNTR).\n\n\nRESULTS\nIsolates showed mutations in the rpoB and katG genes, and the inhA promoter. In rpoB, we found 13 genetic alterations at codons 511, 513, 514, 515, 516, 526 and 531. Forty-six (44.2%) RMP-resistant isolates belonged to codon 531. In katG, there were nine genetic alterations at codons 296, 312, 314, 315, 322, 324 and 351. Fifty-three (51%) INH-resistant isolates belonged to codon 315. Five mutations not previously described were identified in katG: Thr324Ser, Thr314Ala, Ala312Pro, Trp351Stop and deleted G at 296 codon. The Latin American Mediterranean (LAM) (33.7%) and Ghana (30.8%) lineages presented most of the main mutations observed.\n\n\nCONCLUSION\nThis is the first report from Ecuador; it describes five new mutations in katG and indicates that LAM is the most prevalent lineage.