Determination of ANAE and AcP-ase Positivity in the Peripheral Blood of Pregnant Women with Hypothyroidism

Abstract

Objective: Pregnancy is a stress factor for thyroid functions. The most commonly observed thyroid disorder is hypothyroidism. The immune system is affected both by pregnancy and hypothyroidism. This disorder causes a decrease in humoral and cell-mediated immune responses. This study was aimed at the comparison of alpha-naphthyl acetate esterase- and acid phosphatase-positive T lymphocyte distribution between healthy and hypothyroid pregnant women.\xa0\xa0 Methods: Blood samples were taken from healthy and hypothyroid pregnant women between June-December 2016. The sampled subjects included 10 healthy non-pregnant women (HC), 10 healthy pregnant women (HP), and 30 hypothyroid pregnant women in different trimesters of pregnancy (HpPTRI, HpPTRII and HpPTRIII). From each of these groups, six smears were prepared and histologically stained for ANAE and AcP-ase activity and with May Grunwald-Giemsa.\xa0\xa0 Results: The lowest ANAE- and AcP-ase-positive T lymphocyte percentages were detected in Group HP, and these percentages were higher in the hypothyroid pregnant groups, compared to Group HC (P<0.05). Based on PBL counts, the lowest lymphocyte and highest neutrophil leukocyte rates were detected in the pregnant groups. Hypothyroid pregnant women were determined to have monocytic leukocyte rates lower than those of Group HC and Group HP (P<0.05).\xa0\xa0 Conclusions: In this study, it was concluded that significant alterations had occurred in the PBL and ANAE- and AcP-ase-positive T lymphocyte counts of Group HP and the hypothyroid pregnant groups. These findings provide important data about the immunity of hypothyroid pregnant women.