Plasma Levels of Glycohydrolase Activities in Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Patients.

Abstract

In recent years, a new method for the evaluation of total plasma antioxidant defenses, known as lag time,3 has proved to be a valid marker of oxidative stress (OS) in different diseases.4 In this study we collected plasma samples from 13 COVID-19 patients and 52 non-COVID-19 patients presenting with COVID-19-like symptoms at the emergency room of Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Policlinico San Donato, San Donato Milanese, Milano, Italy. In the COVID19-positive group, lag-time values showed a significant negative linear correlation with GCR level, suggesting that this parameter could be a good indicator of OS in COVID-19 patients (Figure). [...]there has been little information on the involvement of plasma glycohydrolases in infectious diseases, such as HIV infection.5 In this regard, to our knowledge, our data highlight for the first time an activation of the lysosomal apparatus in SARS-CoV-2 infection. In particular, our results indicate a negative correlation between total plasma antioxidant defenses and GCR, suggesting a link between OS and lysosomal apparatus activation. [...]because of the clinical relevance of OS in COVID-19, the evaluation of both glycohydrolase levels and plasmatic antioxidant defenses could be suggested as a new possible tool for future studies characterizing the role of OS in COVID-19 infection. 1Department of Biomedical Sciences for Health (SCIBIS), Universita degli Studi di Milano, Milan, Italy;2Department of Cardiovascular Anesthesia and Intensive Care, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy;3IRCCS Orthopedic Institute, Galeazzi, Milan, Italy;4O.U. of Clinical Pathology, Department of Pathology and Laboratory Medicine, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy We acknowledge Camilla Mocchi, BSci, for her precious help in the enzymatic assay realization and Judy Baggott, PhD, for English editing.