BACTERIOLOGICAL PROFILE OF BURN PATIENTS AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF BIO FILM FORMING ISOLATES TOWARD ANTIBIOTIC AND PLANT EXTRACTS

Abstract

Objective: Bio film is an association of micro-organisms in which microbial cells adhere to each other on a living or non-living surfaces within selfproduced matrix. Bacteria are the most common microorganism found in a bio film and they are very infectious. Antibiotics are a normal choice to treat bacteria, but because of the bacterial resistance for the usual antibiotics, we aimed to examine some natural plants extracts effect on bio film. Methods: A total of 56 specimens were obtained from patients suffering from burn wound infections. Eight types of bacteria were isolated and identified by standard biochemical tests used for clinical diagnosis. The susceptibilities of all strains against Penicillin G, Trimethoprim, Gentamicin, Vancomycin, Tetracycline and Ciprofloxacin were assessed by Disc-Diffusion method. Assessment of antibacterial activity of some plant extract on bio film forming bacteria was done by agar well diffusion method. Results: 57.14 % of swabs were strong bio film forming bacteria, 25 % were moderate, 10.72 % were weak and 7.14 % were no bio film forming. The single bacterial isolates were 42.86 %, mixed isolates were 57.14 %. The most common isolated pathogen was Pseudomonas aeruginosa (23.41 %) as well as Escherichia coli (19.14 %). The isolates were highly resistant to Penicillin G, Vancomycin, Trimethoprim and Tetracycline (91.0 %, 87 %, 85.7 % and 82.1 %) respectively and sensitive to Ciprofloxacin. The results show that fresh juice of Allium sativum has strong antibacterial activity against the isolate, hot water extract of Myrtus communis has weak antibacterial activity while fresh juice of Allium cepa has no antibacterial activity against the isolates.