Artificial Intelligence Review | 2019
Ameliorating Effect of Ganoderma lucidum on Combined Oral Contraceptive-induced Cardiometabolic Syndrome in Female Guinea Pigs
Abstract
Aim: This study evaluated the effect of Ganoderma lucidum on selected biochemical indices for cardiometabolic risk. Study Design: Case-control study. Place and duration of Study: This study was carried out in the Department of Medical Laboratory Science, Babcock University between December, 2018-May, 2019. Methods: The study included 32 female guinea pigs which were assigned into 4 groups (A-D) with 8 in each group. Group A animals served as control, group B animals received only monophasic combined oral contraceptives (COC), group C animals received monophasic COC and 50mg/kg of G. lucidum, group D animals received monophasic COC and 100 mg/kg of G. lucidum. All the test animals received their treatment once daily through oral gavage for 12 weeks. All animals were Original Research Article Adediji et al.; AIR, 19(4): 1-7, 2019; Article no.AIR.50332 2 sacrificed 24 hours after the last dose was given to the test groups. Blood sample was collected via cardiac puncture. Lipoprotein-associated phospholipase-A2 (Lp-PLA2), high sensitive C reactive protein (hsCRP), insulin, insulin-like growth factor-1 (IGF-1), fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TC) and high density lipoprotein-cholesterol (HDL-C) were determined using spectrophotometric methods and ELISA as appropriate. HOMA-IR was calculated using homeostasis model assessment index. Data obtained were statistically analyzed using ANOVA, post hoc, all values were expressed as mean±standard deviation. P value less than 0.05 was considered significant. Results: We observed that groups B (25%) and C (25%) animals had significantly higher levels of hsCRP, LpPLA2, TC, TG, LDL, FBG, IGF-1 and HOMA-IR when compared with groups A (25%) and D animals (25%) (p<0.05), however there was no significant statistical difference when groups B and C were compared. Also we observed higher levels of HDL in groups C and D animals when compared with groups A and B animals but not significant statistically. Conclusion: The use of G. lucidum stymies the development of COC-induced cardiometabolic syndrome in a dose-dependent manner.