A. Aranda

In vitro evaluation of IgE-mediated hypersensitivity reactions to quinolones

To cite this article: Aranda A, Mayorga C, Ariza A, Doña I, Rosado A, Blanca‐Lopez N, Andreu I, Torres MJ. In vitro evaluation of IgE‐mediated hypersensitivity reactions to quinolones. Allergy 2011; 66: 247–254.

Local allergic rhinitis: Allergen tolerance and immunologic changes after preseasonal immunotherapy with grass pollen

clinical population classified on the basis of ICD-9 codes alone. In particular, the ORMDL3/GSDML locus associations, which have been replicated in many GWA studies composed of carefully recruited subjects, can also be observed in a white population identified via extracted ICD-9 codes from EMRs. Despite the current small sample size of black subjects, it is evident from our data, and consistent with reported GWA studies, that the allelic frequency distribution among black subjects can be markedly different than that among white subjects at some of the loci that are most prominently reported in GWA studies. Thus, continuing to pursue asthma GWA studies in black subjects, as well as subjects of diverse ethnic backgrounds, will likely yield important insights into the genetics of asthma. Despite the disadvantages related to a more incomplete characterization of disease and environmental variables than that provided by traditional epidemiologic and clinical trial populations, iCAP and other general clinical populations are likely to provide a faster and more cost-effective means to conduct genomic studies of asthma. The insights gained through studies of these general clinical populations will complement those of traditional ones. Blanca E. Himes, PhD Barbara Klanderman, PhD Isaac S. Kohane, MD, PhD Scott T. Weiss, MD, MS

Diagnosis of immediate hypersensitivity reactions to radiocontrast media

No consensus exists on the diagnostic approach for immediate hypersensitivity reactions (IHR) to radiocontrast media (RCM). We analyzed the diagnostic value of a skin test (ST), drug provocation test (DPT) and basophil activation test (BAT) in patients with symptoms compatible with IHR to RCM.

Pru p 3 acts as a strong sensitizer for peanut allergy in Spain

Pru p 3 has been suggested to be the primary sensitizing allergen in patients with peanut allergy in the Mediterranean area. We aimed to confirm this hypothesis, studying 79 subjects.

IgE‐mediated hypersensitivity reactions to methylprednisolone

To cite this article: Aranda A, Mayorga C, Ariza A, Doña I, Blanca‐Lopez N, Canto G, Blanca M, Torres MJ. IgE‐mediated hypersensitivity reactions to methylprednisolone. Allergy 2010; 65: 1376–1380.

Fluoroquinolone Photodegradation Influences Specific Basophil Activation

Fluoroquinolones (FQs) are photoreactive drugs, but it is not known whether laboratory light exposure can influence the induction of photoproducts and modify in vitro test results. The basophil activation test (BAT) has proven to be useful for evaluating immunoglobulin E (IgE)-mediated hypersensitivity to FQs, with a higher percentage of positive responders with ciprofloxacin (CIP) than with moxifloxacin (MOX). We studied the effect of laboratory light on CIP and MOX degradation, and drug-protein conjugate formation, and its influence on the BAT for evaluating IgE-mediated hypersensitivity to FQs. The results showed an important decrease in fluorescence emission intensity under light compared to dark conditions for MOX, and that BAT positivity was lower in light (17.9%) than in dark (35.7%) conditions. No changes were found for CIP in either fluorescence emission intensity or BAT results (46.4% in both conditions). We can conclude that light exposure is a critical factor in BAT results when photolabile drugs like MOX are used. Therefore, light is important when interpreting in vitro results.

Basophil response to peanut allergens in the mediterranean area

Methods We included 60 subjects in different groups: Group 1, peanut and peach allergy; Group 2, peanut allergy and good tolerance to peach; Group 3, peach allergy and good tolerance to peanut; Group 4, good tolerance to peach and peanut. The diagnostic was performed by clinical history, skin prick test and/or double blind placebo controlled food challenge. BAT to Arah1, Arah2, Arah3, Arah6, rArah9 and rPrup3 was done using antiIgE and CD63 and cells analyzed in a FACSCalibur flow cytometer.