A. Kottai Muthu

Evaluation of Hypolipidemic Effect of Various Extracts of Whole Plant of Bauhinia purpurea in Rat Fed with High Fat Diet

The objective of the present study was to investigate the hypolipidemic effect of various extracts of whole plant of Bauhinia purpurea in rat fed with high fat diet. The elevated levels of total cholesterol, ester and free cholesterol, phospholipids, triglycerides, low-density lipoprotein, and very low-density lipoprotein due to high fat diet (HFD). The group receiving ethyl acetate extract of Bauhinia purpurea at the dose of 250 mg/kg (Group IV) was significantly (P < 0.001) reduced the lipid profile and lipoprotein levels. A significant (p < 0.001) reduction in HDL-cholesterol was noticed in HFD fed groups (II); however, a significant increased the HDL level was produced by the administration of ethyl acetate extract of Bauhinia purpurea. There was a noticed increase in the body weight in HFD fed group (II), which was significantly (p < 0.001) reduced by the administration of ethyl acetate extract of Bauhinia purpurea. Therefore, it was concluded that the ethyl acetate extract of whole plant of Bauhinia purpurea as definite cardio protective effect against hyperlipidemia.

Isolation and Characterization of Active Components Derived from Whole Plant of Mucuna pruriens (Linn.)

The aim of the present investigation was isolation and characterization of active components derived from whole plant of Mucuna pruriens. The plant were extracted with various solvents (pet. ether, ethyl acetate and methanol), methanol was found to be more active among them. The preliminary phytochemical results revealed that phytosterols, flavonoids and amino acids as active constituents in methanolic extract of Mucuna pruriens. The methanolic extract of Mucuna pruriens was undergone column chromatography with different solvent fractions. Despite, three compounds were isolated from methanolic extract of Mucuna pruriens with the compound 1 was eluted with benzene: Chloroform 90:10, v/v and compound 2 were eluted with eluted with ethyl acetate: methanol 80:20, v/v and then compound 3 were eluted with ethyl acetate: methanol, 70:30, v/v. The structures of the two isolated compounds were characterized by using FT-IR, NMR and Mass spectrophotometric methods. Thus, the compound 1 was characterized as acetate of 3β-Hydroxy-5α-Cholanic acid (C26H38O3), the compound 2 was characterized as 3, 5, 7, 4′-Tetrahydroxy-6-methoxyflavone (C16H12O7) and the compound 3 was characterized as Ethyl 2-amino-5-hydroxy-3, 6, 6-trimethyl heptonate (C12H25NO3). Furthermore, pharmacological studies required for the isolated compounds.