A. O. Barabanova
Russian Academy of Sciences
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Featured researches published by A. O. Barabanova.
Journal of Plant Diseases and Protection | 2004
A. V. Reunov; V. P. Nagorskaya; Larissa Lapshina; Irina M. Yermak; A. O. Barabanova
It is shown that κ/ß-carrageenan obtained from red marine alga Tichocarpus crinitus possesses antiviral activity against Tobacco mosaic virus (TMV). Two days after inoculation of Nicotiana tabacum L. cv. ‘Samsun’ detached leaves with a mixture of TMV (1 μ/ml) and carrageenan (1 mg/ml), the infectivity and virus content in the leaves as well as the intracellular accumulation of virus particles were significantly inhibited in comparison with leaves inoculated with TMV only. The carrageenan applied 24 h prior to inoculation with TMV also exhibited the antiviral effects but to a lower degree than when the virus and polysaccharide were inoculated together. As the infection developed, the inhibitory effects of carrageenan decreased. Treatment of the leaves with carrageenan affected the formation of TMV-specific intracellular inclusions (granular and tubule) known to consist of the virus-encoded protein components of the viral replicase. A characteristic of the infection development in the presence of carrageenan, 4 days after infection of the leaves with TMV, was the predominant formation in cells of granular inclusions famous to appear at early stages of TMV multiplication. At the same time, cells of the leaves inoculated with TMV alone contained for the most part tubule inclusions considered to arise from granular ones on the advanced infection stages. Taken together, our observations indicate that the κ/ß-carrageenan inhibits TMV infection in detached tobacco leaves at early stages.ZusammenfassungEs wurde gezeigt, dass κ/ß-Karagheen, das aus den roten Meeresalgen Tichocarpus crinitus gewonnen wurde, hohe Antivirusaktivität gegen das Tabakmosaikvirus (TMV) besitzt. Zwei Tage nach der Inokulation der Blätter von Nicotiana tabacum L. cv. ‘Samsun’ mit der Mischung TMV (1 μ/ml) und κ/ß-Karagheen (1 mg/ml) werden die Infektionsstufe, der Gehalt an Virus in den Blättern, und auch die Akkumulation von Virusbestandteilen in den Zellen signifikant gehemmt. κ/ß-Karagheen, das 24 h vor der Inokulation mit TMV angewendet wurde, auch Antiviruseffekte, aber weniger als wenn Virus und Polysaccharid gemeinsam inokuliert wurden. Im Laufe der Entwicklung der Infektion wurden die Hemmeffekte von κ/ß-Karagheen immer schwächer. Die Behandlung der Blätter mit κ/ß-Karagheen beeinflusste die Entstehung von TMV-spezifischen intrazellulären Komponenten (granulär und tubulär), die bekanntlich aus den virus-codierten Eiweißkomponenten der Virenreplikase bestehen. Charakteristisches Merkmal der Entwicklung der Infektion in Anwesenheit von κ/ß-Karagheen (4 Tage nach der Inokulation der Blätter mit TMV) war die vorwiegende Bildung der Granular- komponenten in den Zellen, die in den Frühstadien der TMV-Replikation entstehen. Die Zellen der Blätter, die nur mit TMV inokuliert worden waren, enthielten hauptsächlich die tubuläre Komponente, die, wie man vermutet, sich während der fortschreitenden Etappen der Infektion aus den Granular- komponenten bilden. Die Ergebnisse zeigen, dass κ/ß-Karagheen die TMV-Infektion in abgeschnittenen Tabakblättern hemmt.
Journal of Applied Phycology | 2008
A. O. Barabanova; A. S. Shashkov; V. P. Glazunov; Vladimir V. Isakov; T. B. Nebylovskaya; W. Helbert; T. F. Solov’eva; Irina M. Yermak
Sulfated polysaccharides occurring in the red algae Tichocarpus crinitus cell wall were fractionated and purified. NMR and FT-IR spectroscopy analyses revealed that the non-gelling fraction contained a sulfated galactans having a new carrageenan-like structure. It is built with alternatively linked 1,3-linked β-D-galactopyranosyl-2,4-disulphates and 1,4-linked 3,6-anhydro-α-D-galactopyranosyl residues. Minor amounts of its biosynthetic precursor were detected in a water-extracted specimen. Brief analysis of rheological and biological properties of the non-gelling fraction was carried out. The carrageenan-like polysaccharide from T. crinitus displayed the properties of “random coil” polymer at high temperature, and possesses high anticoagulant activity at low concentration.
Carbohydrate Polymers | 2013
Ekaterina V. Sokolova; Evgeniy Anatolievich Chusovitin; A. O. Barabanova; S.A. Balagan; N.G. Galkin; Irina M. Yermak
In the present article, the atomic force microscopy was applied to investigate macromolecular structures of various carrageenan types including hybrid polysaccharides (κ-, κ/β-, κ/ι-, λ-, and X-carrageenans) depending on polysaccharide concentration. The structures dependence on a polysaccharide concentration also was focused. κ-Carrageenan forms both single and two stranded structures at a low concentration. At high concentrations κ-, κ/β-, and κ/ι-carrageenans form fibrous network-like structures by a side-by-side association type at the same time for κ/ι-carrageenan end-to-end association type also was found. Comparably to κ-carrageenan, κ/β-carrageenan network was more open with coarser fibers while κ/ι-carrageenan structure is characterized with a more flexible network. Honeycombed structures due to end-to-end and side-by-side association types were observed for X-carrageenan, while λ-carrageenan formed honeycombed structures only at high concentrations. In order to investigate topographical parameters of the carrageenans macromolecular structure a new method of the autocorrelation function analysis was used for the first time.
Biology Bulletin | 2008
V. P. Nagorskaya; A. V. Reunov; L. A. Lapshina; Irina M. Yermak; A. O. Barabanova
The influence of κ/β-carrageenan from red marine alga Tichocarpus crinitus on the development of tobacco mosaic virus (TMV) infection in Xanthi-nc tobacco leaves was studied. It was shown that the number of necrotic lesions on the leaves inoculated with the mixture of TMV (2 μg/ml) and carrageenan (1 mg/ml) was reduced by 87%, compared to the leaves inoculated with the virus only. The suppression of virus infection was also observed when leaves were treated with carrageenan 24 h before or 24 h after leaf inoculation with TMV; however, in these cases, suppression was less evident than after inoculation with the virus-polysaccharide mixture. It is supposed that the antiviral activity of carrageenan applied together with TMV may be explained by its action not only on the plant but also on the virus itself. The inhibitory effect of carrageenan pretreatment can be explained by its favorable effect on tissue resistance to infection. The suppression of this resistance by actinomycin D indicates that carrageenan functions via its action on the cell genome.
Bulletin of Experimental Biology and Medicine | 2011
Ekaterina V. Sokolova; A. O. Barabanova; V. A. Homenko; T. F. Solov’eva; R. N. Bogdanovich; Irina M. Yermak
Antioxidant properties of structurally different sulfated polysaccharides (carrageenans) were studied in vitro and ex vivo. Ferric reducing antioxidant activity of carrageenans and their inhibitory effects on hydroxyl radicals and superoxide anion radicals were demonstrated in vitro. Activity of carrageenans depends on the polysaccharide structure. Carrageenans stimulate catalytic activity of SOD from donor erythrocyte.
Carbohydrate Polymers | 2015
Alexandra A. Kalitnik; Pavel A. Marcov; Stanislav D. Anastyuk; A. O. Barabanova; Valeriy P. Glazunov; Sergey V. Popov; Yuriy S. Ovodov; Irina M. Yermak
Structural peculiarities of low molecular weight (LMW) sample obtained by mild acid hydrolysis of κ-carrageenan from Chondrus armatus was investigated by a rapid mass spectrometric method. The selected conditions allowed avoiding excess destruction of 3,6-AnGal residues that was shown by using tandem (MS/MS) mode. Main oligosaccharide fraction with molecular weight 2.3 kg/mol, obtained by mild acid hydrolysis was chosen for the analysis. It was shown that fragments with even degree of polymerization (DP) were mostly built of (-G4S-DA-)n repeating unit, n=1-5. Some fragments with odd DP were shown to contain -DA2S- insertions, being the fragments of ι-type blocks, which were randomly distributed along the polysaccharide chain as single insertions. The anti-inflammatory activity (on acetic acid-induced colitis in mice) of the initial polymer and its derivatives was studied. The anti-inflammatory effect of the polymer was observed at a dose of 5mg/kg. Polysaccharide decreased the degree of colon damage more than twice and area of damage in 40%.
Chemistry of Natural Compounds | 2012
A.V. Volod’ko; V. N. Davydova; A. O. Barabanova; T. F. Solov’eva; I. M. Ermak
The formation process of soluble chitosan (C)/κ-carrageenan (K) complexes was studied. It was shown that soluble complexes were obtained preferentially by mixing the starting components at given ratios whereas soluble complexes were formed only over a narrow range of starting-component ratios by titration of K with C. The formation of C/K polyelectrolyte complexes was confirmed by gel-permeation chromatography and centrifugation in a Percoll gradient. The formation process of C/K complexes depended on the C molecular weight and the concentrations and ratios of starting polysaccharides. It was shown that unbound components in addition to the complex remained in the mixture upon mixing carrageenan with high-molecular-weight C (C-HM) in 1:1.5 and 1:30 ratios (mol/mol) whereas low-molecular-weight C (C-LM) was bonded completely to the polyanion.
Biochemistry | 2011
L. A. Lapshina; V. P. Nagorskaya; A. V. Reunov; A. O. Barabanova; N. M. Shevchenko; Irina M. Yermak; T. N. Zvyagintseva; L. A. Elyakova
The activities of hydrolases (acid phosphatase, RNase, and proteases) in healthy and tobacco mosaic virus-infected leaves of Nicotiana tabacum L. var. Samsun, both untreated and treated with polysaccharides (PS) (1,3;1,6-β-D-glucan, fucoidan, and κ/β-carrageenan), were determined. The PS lead to substantial increase in the hydrolase level. The percentage of viral particles undergoing destructive change also increases in leaves treated with PS 24 h before infection. We suppose that the PS-mediated hydrolase activation promotes intracellular destruction of the viral particles and, thus, comprises one of the PS-induced protective mechanisms limiting intracellular viral accumulation.
Biology Bulletin | 2010
V. P. Nagorskaya; A. V. Reunov; L. A. Lapshina; I. M. Ermak; A. O. Barabanova
The effect of κ/β-carrageenan from red alda Tichocarpus crinitus on the development of a potato virus X (PVX) infection in the leaves of Datura stramonium L. has been studied. The treatment of leaves with carrageenan stimulates a protein synthesis in the cells, causing an increase in the size of nucleoli and in the number of mitochondria and membranes of the rough endoplasmic reticulum. At the same time, such treatment slightly stimulates lytic processes, causing an increase in the number of smooth endoplasmic reticulum cisternae, dictyosomes, and cytoplasmic vacuoles and the formation of cytoplasmic electron-transparent zones. The carrageenan-induced stimulation of lytic processes results in the destruction of viral particles and can be considered as one of the defense mechanisms, preventing the intracellular accumulation of virus. The carrageenan-stimulated formation of PVX-specific laminar structures, able to bind viral particles and, therefore, prevent their intracellular translocation and reproduction, represents another carrageenan-induced mechanism of the antiviral defense in plant cells.
Advanced Materials Research | 2013
A. O. Barabanova; Andrei Shibaev; V.S. Molchanov; O.E. Philippova; Alexei R. Khokhlov
This paper describes two methods of preparation of magnetic fluids based on associated polymers. The first method is ultrasonic (US) dispersing of magnetite microparticles (MP) in aqueous solutions of associated polymers such as hydrophobically modified polyacrylamides (HM PAAm) – terpolymers of acrylamide (AAm), sodium acrylate (SA) and N-dodecylacrylamide (DDAA). According to the second method the magnetic fluids were formed by addition of magnetic polymers, synthesized by means of a micellar copolymerization of AA, SA and DDAA in the presence of MP, into water. The investigation of the rheological properties has shown that magnetic fluids obtained can be reversibly transformed in physical gels in magnetic field (MF).