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Water Research | 1989

Isolation of wild-type hepatitis A virus from the environment

M Divizia; P De Filippis; A. Di Napoli; A. Venuti; R. Perez-Bercoff; A Pana

Abstract Three isolates of human hepatitis A virus (HAV) were cultivated directly from polluted Tiber water on Frp/3 cells. From 50 to 125 l. of water were prefiltered through 10 and 1 μm polygard filters and concentrated using a molecular ultrafiltration system. The prefilters were washed with 500 ml of beef-extract pH 9.0–9.5 to remove any adsorbed virus. The washing buffer was acid-pH precipitated or concentrated by ultrafiltration. Each sample was neutralized with a pool of antibodies against all the enteroviruses excluding hepatitis A virus. Three samples obtained from prefilters were scored as positive. Hepatitis A virus was identified by commercial ELISA test, indirect immunofluorescence, neutralization test, performed with polyclonal or monoclonal antibody, and by hybridization test with cDNA probes.


European Journal of Epidemiology | 1988

Genital herpes infection in outpatients attending a sexually transmitted disease clinic in Italy.

Alfonso Mele; Franco E; Federico Caprilli; G. Gentili; B. Capitanio; E. Crescimbeni; A. Di Napoli; Laura Zaratti; Susanna Conti; Rosamaria Corona; Giovanni Rezza; A Pana; Paolo Pasquini

Prevalence of Herpes Simplex, type 2, specific antibodies was estimated in sexually transmitted disease outpatients: 783 heterosexuals and 158 homosexual-bisexuals. The anti-HSV-2 prevalence rates were 69% in the homosexual-bisexuals and 35% in the heterosexuals. In both groups positive association with age of anti-HSV-2 prevalence was found: only in the homosexual-bisexuals negative as sociation with education level was detected. No difference exists between the two groups regarding the symptomatic/asymptomatic ratio of HSV-2 infection.


European Journal of Epidemiology | 1987

Detection of Hepatitis A virus in the stools of healthy people from endemic areas

P. de Filippis; M Divizia; Alfonso Mele; B. Adamo; A Pana

One-hundred-ninety-two stool samples were tested for the presence of human Hepatitis A antigen. Sixteen of these were also evaluated for the presence of infectious virus. All samples were obtained from young and apparently healthy people from endemic areas for Hepatitis A disease. The isolation of the infectious virus from these stools demonstrates clearly the wide diffusion of the virus in these areas, and its transmission by the oral-fecal route.


The Lancet | 1987

ISOLATION OF HEPATITIS A VIRUS FROM POLLUTED RIVER WATER ON FRP/3 CELLS

A Pana; M Divizia; P De Filippis; A. Di Napoli


prevenzione e sanita’ pubblica: modelli organizzativi e buone pratiche | 2009

Legionella pneumophila: quattro anni di monitoraggio in una struttura termale del lazio

P De Filippis; F Damiani; S Iannotti; R Gabrieli; M Maurici; C Mozzetti; A Pana


Igiene e sanità pubblica | 2007

La qualità microbiologica delle acque utilizzate in emodialisi in vari ospedali e case di cura del lazio sulla base di controlli effettuati nell’arco di cinque anni

P De Filippis; G Spitaleri; F Damiani; A Pana


‘La prevenzione per la promozione della salute e l’integrazione dei popoli’ | 2006

RICERCA DI LEGIONELLA IN UN CENTRO TERMALE DEL LAZIO

P De Filippis; G Spiteleri; F Damiani; A Pana


‘L’igienista nelle scelte strategiche e operative’, | 2005

CONTAMINAZIONE IN AMBIENTE ODONTOIATRICO DETERMINATA DAGLI STRUMENTI PER LA DETARTRASI

R Gabrieli; C Salvatori; P De Filippis; G Bandini; M Divizia; A Pana


Igiene e sanità pubblica | 2005

Ricerca di pseudomonas aeruginosa nelle acque di dialisi quale ulteriore parametro microbiologico di qualità

P De Filippis; A Spinaci; G Spitaleri; F Damiani; A Pana


Conferenza nazionale di sanità pubblica "L’igienista nelle scelte strategiche e operative" | 2005

Controlli microbiologici ambientali nei blocchi operatori con particolare riguardo alla ricerca di legionella

P De Filippis; A Spinaci; M Di Porto; F Damiani; A Pana

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R Gabrieli

University of Rome Tor Vergata

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Alfonso Mele

Istituto Superiore di Sanità

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A. Venuti

Sapienza University of Rome

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C Mozzetti

University of Rome Tor Vergata

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Franco E

University of Rome Tor Vergata

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G. Gentili

Istituto Superiore di Sanità

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Giovanni Rezza

Istituto Superiore di Sanità

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Laura Zaratti

University of Rome Tor Vergata

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Paolo Pasquini

Istituto Superiore di Sanità

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R. Perez-Bercoff

Sapienza University of Rome

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