A. S. Lino

Metal bioaccumulation in consumed marine bivalves in Southeast Brazilian coast.

This work aimed to investigate metal bioaccumulation by mussels (Perna perna) and Lions Scallop (Nodipecten nodosus) farmed in tropical bays, in order to estimate spatial and temporal variation in the exposure to these elements, as well as human health risk. The concentration of each measured element was considered for this evaluation, using maximum residue level (MRL) in foods established by the Brazilian (ANVISA), American (USFDA) and European Communities (EC) legislations. Values for estimated daily ingestion (EDI) were determined for metals intake through mussel and scallop consumption. These estimates were compared with the reference value of (PTDI) proposed by World Health Organization (WHO). Trace elements concentration was measured on ninety mussels P. perna (tissue) and ninety Lions Scallop N. nodosus (muscle and gonad) reared in four different tropical areas of the Southeast Brazilian coast, between 2009 and 2010. Zinc (Zn), Iron (Fe), Copper (Cu), Manganese (Mn), Chrome (Cr), Nickel (Ni), Cadmium (Cd) and Lead (Pb) concentrations were measured by flame atomic absorption spectrometry after acid mineralization. Cd and Mn were more efficiently bioaccumulated by scallops than mussels and the opposite was found for Fe, Cu and Ni. Guanabara Bay and Sepetiba Bay were considered the most impacted between ecosystems studied. Higher Cd values in Arraial do Cabo in the other sites studied were associated with upwelling that occurs in the region. Consumption of both species cannot be considered safe, because the Cu and Cr concentrations, in accordance with the limits established by the Brazilian Agency (ANVISA). On the other hand, any EDI value exceeded the corresponding value of the PTDI, proposed by World Health Organization (WHO).

Zirconium phosphate organically intercalated/exfoliated with long chain amine

Nanolayered zirconium phosphate was synthesized by the direct precipitation reaction method, and it was organically modified with long chain amine (octadecylamine) at different amine:phosphate ratios (0.5:1, 1:1, and 2:1). Both zirconium phosphate and amine were dispersed in a 2:1 ethanol/water solution. Infrared spectroscopy (FT-IR), thermogravimetry (TG/DTG), differential scanning calorimetry, wide-angle X-ray diffraction (WAXD), and scanning electron microscopy (SEM) were used for characterization. The amine:phosphate ratio regulated the amine insertion in the zirconium phosphate lamellae, as observed by infrared bands, thermal curves, and SEM images. Different TG/DTG degradation temperatures evidenced adsorbed and bonded amine molecules in the phosphate lamellae. Calorimetric results indicated several amine melting temperatures suggesting different crystal arrangements in the phosphate galleries. The presence of octadecylamine changed the crystallographic features of the zirconium phosphate as observed in WAXD.

High density polyethylene and zirconium phosphate nanocomposites

Nanocomposite based on high density polyethylene (HDPE) and layered zirconium phosphate organically modified with octadecylamine (ZrPOct) was obtained through melt processing. The ZrPOct was synthesized by precipitation and modified by suspension and sonication procedures. The initial and maximum degradation temperatures (Tonset and Tmax) were increased. A slight decrease of crystallinity degree was detected. Reduction of elastic modulus and elongation at break were noticed. The lamellar spacing was increased (3.3 times higher). The storage modulus decreased and low field nuclear magnetic resonance (LFNMR) revealed an increasing of molecular mobility. The presence of octadecylamine enhanced the entrance of HDPE in the ZrPOct galleries. Several characteristics of HDPE were changed indicating that intercalation was successful. All results indicated that partially intercalated and/or exfoliated nanocomposite was achieved.

Mercury and selenium in fishes from the Tapajós River in the Brazilian Amazon: An evaluation of human exposure

This work aimed to evaluate associated risks of fish consumption to human health, concerning mercury (Hg) and selenium (Se) concentrations in fish species largely consumed in the Tapajós River basin in the Brazilian Amazon. Total mercury (THg), methylmercury (MeHg) and Se concentrations were measured in 129 fish specimens from four sites of the Tapajós River basin. Estimated daily intake (EDI) of Hg and Se were reported regarding fish consumption. EDI were compared with the reference value of provisional tolerable daily intake proposed by the World Health Organization (WHO). Se:Hg ratios and selenium health benefit values (Se HBVs) seem to offer a more comprehensive fish safety model. THg concentrations in fishes ranged from 0.03 to 1.51 μg g-1 of wet weight (w.w.) and MeHg concentrations ranged from 0.02 to 1.44 μg g-1 (w.w.). 80% of the samples were below the value of Hg recommended by the WHO for human consumption (0.5 μg g-1 w.w.). However, Hg EDI exceeded the dose suggested by the United States Environmental Protection Agency (0.1 μg kg-1 day-1), due to the large level of fish consumption in that area. Se concentrations in fishes ranged from 0.02 to 0.44 μg g-1 w.w. An inverse pattern was observed between Hg and Se concentrations in the trophic chain (highest levels of Se in the lowest trophic levels). The molar ratio Se:Hg and Se HBVs were higher in iliophagous and herbivorous fishes, which is noteworthy to reduce toxic effects of Hg contamination. For planktivores, the content of Se and Hg was almost equimolar. Carnivorous fishes - with the exception of Hemisorubim platyrhynchos and Pseudoplatystoma fasciatum -, showed Se:Hg ratios <1. Thus, they do not act as a favorable source of Se in the diet. Therefore, reduced intake of carnivorous fishes with preferential consumption of iliophages, herbivores and, to some extent, even planktivores should be promoted as part of a healthier diet.

Far-reaching cytogenotoxic effects of mine waste from the Fundão dam disaster in Brazil

On November 2015, one of Brazils most important watersheds was impacted by the mine waste from Fundão dam collapse in Mariana. The mine waste traveled over 600 km along the Doce River before reaching the sea, causing severe devastation along its way. Here we assessed trace element concentrations and cytogenotoxic effects of the released mine waste. Water samples were collected along the Doce River ten days after the disaster in two impacted sites and one non-impacted site. Sampling points were located hundreds of kilometers downstream of the collapsed dam. Water samples were used for trace element quantification and to run an experiment using Allium cepa to test cytogenotoxicity. We found extremely high concentrations of particulate Fe, Al, and Mn in the impacted sites. We observed cytogenotoxic effects such as alterations in mitotic and phase indexes, and enhanced frequency of chromosomal aberrations. Our results indicate interferences in the cell cycle in impacted sites located hundreds of kilometers downstream of the disaster. The environmental impacts of the dam collapse may not only be far-reaching but also very likely long-lasting, because the mine waste may persist in the Doce River sediment for decades.