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Dive into the research topics where A. Van de Weghe is active.

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Featured researches published by A. Van de Weghe.


web science | 1995

THE PIGMAP CONSORTIUM LINKAGE MAP OF THE PIG (SUS SCROFA).

Alan Archibald; Chris Haley; J. F. Brown; S. Couperwhite; H A McQueen; D. Nicholson; W. Coppieters; A. Van de Weghe; A. Stratil; Anne Katrine Winterø; Merete Fredholm; N. J. Larsen; Vivi Hunnicke Nielsen; Denis Milan; N. Woloszyn; Annie Robic; M. Dalens; Juliette Riquet; J. Gellin; J. C. Caritez; G. Burgaud; L. Ollivier; J. P. Bidanel; Marcel Vaiman; Christine Renard; H. Geldermann; R. Davoli; D. Ruyter; E. J. M. Verstege; M.A.M. Groenen

A linkage map of the porcine genome has been developed by segregation analysis of 239 genetic markers. Eighty-one of these markers correspond to known genes. Linkage groups have been assigned to all 18 autosomes plus the X Chromosome (Chr). As 69 of the markers on the linkage map have also been mapped physically (by others), there is significant integration of linkage and physical map data. Six informative markers failed to show linkage to these maps. As in other species, the genetic map of the heterogametic sex (male) was significantly shorter (∼16.5 Morgans) than the genetic map of the homogametic sex (female) (∼21.5 Morgans). The sex-averaged genetic map of the pig was estimated to be ∼18 Morgans in length. Mapping information for 61 Type I loci (genes) enhances the contribution of the pig gene map to comparative gene mapping. Because the linkage map incorporates both highly polymorphic Type II loci, predominantly microsatellites, and Type I loci, it will be useful both for large experiments to map quantitative trait loci and for the subsequent isolation of trait genes following a comparative and candidate gene approach.


Mammalian Genome | 1997

A medium-density genetic linkage map of the bovine genome

W. Barendse; D. Vaiman; Stephen J. Kemp; Yoshikazu Sugimoto; S. M. Armitage; J. L. Williams; H. S. Sun; A. Eggen; Morris Agaba; S. A. Aleyasin; Mark Band; M. D. Bishop; J. Buitkamp; K. Byrne; F. Collins; L. Cooper; W. Coppettiers; B. Denys; R. D. Drinkwater; K. Easterday; C. Elduque; Sean Ennis; G. Erhardt; L. Ferretti; N. Flavin; Q. Gao; Michel Georges; R. Gurung; B. Harlizius; G. Hawkins

A cattle genetic linkage map was constructed which covers more than 95 percent of the bovine genome at medium density. Seven hundred and forty six DNA polymorphisms were genotyped in cattle families which comprise 347 individuals in full sibling pedigrees. Seven hundred and three of the loci are linked to at least one other locus. All linkage groups are assigned to chromosomes, and all are orientated with regards to the centromere. There is little overall difference in the lengths of the bull and cow linkage maps although there are individual differences between maps of chromosomes. One hundred and sixty polymorphisms are in or near genes, and the resultant genome-wide comparative analyses indicate that while there is greater conservation of synteny between cattle and humans compared with mice, the conservation of gene order between cattle and humans is much less than would be expected from the conservation of synteny. This map provides a basis for high-resolution mapping of the bovine genome with physical resources such as Yeast and Bacterial Artificial Chromosomes as well as providing the underpinning for the interpolation of information from the Human Genome Project.USDA-MARC family and data for validating this family. P. Creighton, C. Skidmore, T. Holm, and A. Georgoudis provided some validation data for the BOVMAP families. R. Fries, S. Johnson, S. Solinas Toldo, and A. Mezzelani kindly made some of their FISH assignments available before publication. We wish to thank all those researchers who kindly sent us probes and DNA primers.


Mammalian Genome | 1996

A detailed physical map of the porcine major histocompatibility complex (MHC) class III region: comparison with human and mouse MHC class III regions.

Luc Peelman; Patrick Chardon; Marcel Vaiman; Marc Mattheeuws; A. Van Zeveren; A. Van de Weghe; Y. Bouquet; R. D. Campbell

A detailed physical map of the porcine MHC class III region on Chr 7 was constructed with a panel of probes in a series of hybridizations on genomic pulsed field gel electrophoresis (PFGE) Southern blots. A precise organization of the 700-kb segment of DNA between G18 and BAT1 can now be proposed, with more than 30 genes mapped to it. Comparison of this region with homologous regions in human and mouse showed only minor differences. The biggest difference was observed in the CYP21/C4 locus with only one CYP21 gene and one C4 gene found, whereas in human and mouse these genes are duplicated. These results show the class III region is very well conserved between pig, human, and mouse, in contrast with the class I and class II regions, which seem more prone to rearrangements.


Mammalian Genome | 1994

A porcine minisatellite located on chromosome 14q29.

W. Coppieters; C. Zijlstra; A. Van de Weghe; A.A. Bosma; Luc Peelman; A. Van Zeveren; Y. Bouquet

Microsatellites or short tandem repeats (STR) have undoubtedly become the most successful class of anonymous genetic markers. They can be typed very efficiently by means of PCR (Weber and May 1989; Litt and Luty 1989) and are almost randomly distributed in mammalian genomes (Stallings et al. 1991; Winterr et al. 1992). In the pig genome, they are underrepresented in the telomeric regions (Winterr et al. 1992). Minisatellites, on the other hand, have become less attractive in linkage studies. This is not only owing to the laborious typing by Southern blot hybridization, but also because there are indications for a nonrandom distribution in the human genome towards proterminal regions (Royle et al. 1988). However, highly polymorphic minisatellites located at the telomeres could complement a microsatellite-based map, as end points for chromosomes. Thus far, only two porcine minisatellites have been described (Coppieters et al. 1990; Davies et al. 1992). Here we report the isolation, partial sequencing, and localization of a new pig minisatellite locus. A size-selected library containing genomic pig DNA fragments ranging from 3 to 4.5 kb was constructed in pUC 18:500 ~tg genomic DNA was digested to completion with MboI and separated on a 0.8% agarose gel. The 2 to 23-kb fragments were electroeluted and subsequently concentrated to 30 ~tl by ethanol precipitation. This fraction was separated a second time on a 1% Seaplaque agarose gel (FMC, Rockland, USA). The agarose was sliced and digested with Gelase (Epicentre Technologies, Madison, Wis., USA). The digested agarose slices that contain the right-sized fragments were used in a ligation reaction with BamHI-digested and dephosphorylated pUC 18 vector. The ligation mix was transformed in DH5c~ high-efficiency competent E. coli cells (BRL, Gaithersburg, Md., USA) and plated at low density on LB agar plates containing Xgal, IPTG, and ampiciline. A total of 2400 white colonies was transferred to microtiterplates and grown overnight in 150 ~tl Terrific Broth.


Comparative Biochemistry and Physiology B | 1982

The vitamin D binding protein in domestic animals

A. Van de Weghe; A. Van Zeveren; Y. Bouquet

Abstract 1. 1. The Gc-fraction in plasma or serum of different animal species was determined and identified by PAGE, autoradiography and two dimensional electrophoresis. 2. 2. Large differences in allele frequencies of populations of some domestic species support the interest in studies for polymorphism as a useful tool in ethnographic description, on the analogy of human genetic population research.


Comparative Biochemistry and Physiology B | 1995

PIG PLASMA ALPHA-PROTEASE INHIBITORS PI2, PI3 AND PI4 ARE MEMBERS OF THE ANTICHYMOTRYPSIN FAMILY.

A. Stratil; D. Čižová-Schröffelová; E Gabrisova; M. Pavlik; W. Coppieters; Luc Peelman; A. Van de Weghe; Y. Bouquet

Three related alpha-protease inhibitors, PI2 I, PI3 C and PI4 C2, of blood serum of the pig (Sus scrofa) were isolated. PI2 I inhibited both trypsin and chymotrypsin; PI3 C and PI4 C2 strongly inhibited chymotrypsin, but did not significantly inhibit trypsin. By using SDS-PAGE, the three proteins were found to be composed of single polypeptide chains, and molecular weights were 63,000 for PI2 I, 58,000 for PI3 C and 64,000 for PI4 C2. All three proteins were shown to be glycoproteins. In PI3 C, eight sialic acid residues were found, and in PI4 C2 (similarly as in PI2 F) 10-11 residues were found. Amino acid composition as well as N-terminal sequences of the three proteins were very similar, indicating close homology. Comparison of these partial amino acid sequences with the cDNA-deduced amino acid sequence of pig alpha-antichymotrypsin (AACT; Buchman, 1989, GenBank, Accession No. M29508) revealed great similarities, the sequence of PI2 I being virtually identical with the pig AACT. On the basis of all available results, PI2 is proposed to be pig AACT, an orthologue of human AACT.


Biochemical Genetics | 1984

Heterogeneity of Es-1 esterases in the rabbit (Oryctolagus cuniculus)

Hugo J. Bellen; A. Van de Weghe; Y. Bouquet; L.F.M. van Zutphen

Analysis of the Es-1 system in the rabbit with polyacrylamide gel electrophoresis (PAGE) revealed a high degree of individual variation. In the liver the number of esterase bands found in the Es-1 region of the gels ranged from 2 to 16. The results indicate that one locus with three alleles is responsible for all of the esterase bands in the Es-1 region. The most plausible explanation for the observed heterogeneity is that each of the alleles codes for a protein (MW 65,000±2000) that is changed by posttranslational modifications, thus giving rise to two to five monomeric enzymes with esterase activity. Polymerization of these monomers then results in 1–11 dimers. Based on similarities with mouse Es-9, chromosomal homology between rabbit Es-1 and mouse Es-9 is proposed.


Livestock Production Science | 1983

Genetic variability expressed by marker-systems in two Belgian pig populations

A. Van Zeveren; Y. Bouquet; A. Van de Weghe; H. Varewyck; J Hojny

Abstract The genetic structure and the variability of 2 Belgian pig breeds, Landrace and Pietrain, were investigated by means of blood marker-systems. The gene frequencies were estimated at 35 loci in a sample of 612 Belgian Landrace and 304 Pietrain pigs. The average heterozygosity is about 34% in Landrace and 28.5% in Pietrain. Various genetic distances were computed and they fluctuate between 0.0624 for the minimum Dm and 0.1309 for the maximum D′, values which are fairly high for a comparison of local breeds within a species. A value of 0.1340 is estimated for the mean coefficient of relationship.


Comparative Biochemistry and Physiology B | 1981

Polymorphism in blood substances of the cat

A. Van de Weghe; Y. Bouquet; D. Mattheeuws; A. Van Zeveren

Abstract 1. 1. In serum and erythrocytes of domestic cats (Felis Catus), genetic variation was detected by starch and polyacrylamide gel electrophoresis. 2. 2. Gc, Tf and X-protein in the serum and 6-PGD, PHI and aryl-Es in the hemolysates are presumed to be under the control of 2 alleles, whereas a second Es in the red cells is postulated to be a codominant system, controlled by 3 alleles. 3. 3. The distribution of the observed phenotypes in most of the systems fits closely to the distribution, expected by the law of random mating, so that a hereditary transmission can be accepted for the 7 polymorphic systems under scope.


Comparative Biochemistry and Physiology B | 1988

The homology between the serum proteins Po2 in pig, Xk in horse and α1B-glycoprotein in human

A. Van de Weghe; W. Coppieters; G. Bauw; J. Vandekerckhove; Y. Bouquet

Abstract 1. 1. Pig serum Po2 protein and horse Xk protein were purified by FPLC, non-denaturing 2D agarose-PAGE and 2D IPG-PAGE. 2. 2. The separated fractions were electroblotted to poly (4- vinyl -N- methylpyridinium iodide ) coated GF/C glass fiber sheets. 3. 3. The partial amino acid sequences and amino acid compositions of different genetic variants of the proteins were determined. 4. 4. The results proved that previously reported polymorphic serum post-albumins in each of these species were homologous to human plasma α 1 B-glycoprotein .

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Marcel Vaiman

Institut national de la recherche agronomique

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A. Stratil

Academy of Sciences of the Czech Republic

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B. Denys

University of Liège

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