Aaron D. Shultz

Chemical excretions of angled bonefish Albula vulpes and their potential use as predation cues by juvenile lemon sharks Negaprion brevirostris

Bonefish Albula vulpes (n = 7) exercised to exhaustion and air exposed for 1 min as part of a catch-and-release angling event were found to excrete both ammonia and urea, but cortisol and lactate were below detectable levels. Urea made up a greater proportion of total nitrogen excretion from these fish at all time points following an angling event. When captive juvenile lemon sharks Negaprion brevirostris (n = 12) were exposed to a 30 s pulse of these chemicals [ammonia (500 mM), cortisol (20 µg l(-1) ), lactate (6 mM) or urea (3 mM)], they showed a significant reduction in the frequency of resting behaviours when exposed to ammonia and urea than when exposed to control water. It appears that products excreted by A. vulpes, particularly ammonia and urea, may provide an olfactory cue for the post-release predation of A. vulpes by N. brevirostris during catch-and-release angling events.

Seasonal blood chemistry response of sub-tropical nearshore fishes to climate change

This experiment uses environmental challenges associated with global climate change to assess the stress response of mangrove fish (bonefish, checkered puffer, and yellowfin mojarra) in two seasons. Identifying species that are most susceptible to environmental stressors will enhance our ability to predict change in ecosystems.

Puffed and bothered: Personality, performance, and the effects of stress on checkered pufferfish

Although consistent individual-level differences in behaviour are widespread and potentially important in evolutionary and ecological processes, relatively few studies focus on the physiological mechanisms that might underlie and regulate these individual-level differences in wild populations. We conducted experiments to determine whether checkered pufferfish (Sphoeroides testudineus), which were collected from a dynamic (in terms of depth and water temperature) tidal mangrove creek environment in The Bahamas, have consistent individual-level differences in locomotor activity and the response to a simulated predator threat, as well as swimming performance and puffing in response to stressors. The relationships between personality and performance traits were evaluated to determine whether they represented stress-coping styles or syndromes. Subsequently, a displacement study was conducted to determine how personality and performance in the laboratory compared to movements in the field. In addition, we tested whether a physiological dose of the stress hormone cortisol would alter individual consistency in behavioural and performance traits. We found that pufferfish exhibited consistent individual differences in personality traits over time (e.g., activity and the duration of a response to a threat) and that performance was consistent between the lab and the natural enclosure. Locomotor activity and the duration of startled behaviour were not associated with swimming and puffing performance. Locomotor activity, puffing performance, and swimming performance were not related to whether fish returned to the tidal creek of capture after displacement. Similarly, a cortisol treatment did not modify behaviour or performance in the laboratory. The results reveal that consistent individual-level differences in behaviour and performance were present in a population from a fluctuating and physiologically challenging environment but that such traits are not necessarily correlated. We also determined that certain individual performance traits were repeatable between the lab and a natural enclosure. However, we found no evidence of a relationship between exogenous cortisol levels and behavioural traits or performance in these fish, which suggests that other internal and external mechanisms may underlie the behaviours and performance tested.

Impacts of sun protection on feeding behavior and mucus removal of bonefish, Albula vulpes

Catch-and-release angling is growing as a tool for the conservation of fish stocks because it assumes that the impacts of angling are negligible. However, many studies have shown that catch-and-release can be stressful to the fish and even result in mortality. Bonefishing represents a popular catch-and-release fishery in the tropics and subtropics, with most anglers spending 6+ hours per day in full sunlight. To protect themselves, anglers typically employ sun protection in the form of liquid sunscreen and UV-blocking clothing. Exposure to chemicals contained in sunscreens may impose additional stressors on fish that are handled and subsequently released. In this study we conducted two separate experiments in the lab facilities in Cape Eleuthera, Bahamas. The first examined bonefish feeding behaviors in response to bait handled with zinc-based sunscreen, oxybenzone-based sunscreen, and no coating on the researcher’s hands. The second experiment quantified the effects of sunscreens and UV blocking gloves on the removal of fish’s protective mucus layer as a result of handling. We did not observe evidence of a change in feeding behavior when bait was handled with hands covered in sunscreen compared to wet hands. However, there was an increase in removal of protective mucus of bonefish when researcher’s hands were coated in oxybenzone containing sunscreen compared to researchers handling fish with wet hands. The results of this study indicate wet hands are the best way to handle fish when participating in catch-and-release angling.

Potential consequences of angling on nest-site fidelity in largemouth bass

Breeding site fidelity has evolved in many vertebrate taxa, suggesting both that site selection has an important influence on fitness potential and that the decision to reuse a nesting site is related to the individual’s prior nesting success at that location. For a species that provides parental care, such as the Largemouth Bass Micropterus salmoides, catch-and-release angling impacts individual nesting success and fitness through physiological disturbance and by removing the nest-guarding male from its brood, thereby allowing temporary access to eggs and hatchlings by brood predators. To assess the impact of catch-and-release angling on nest site fidelity, we compared the consequences of angling on individually marked (i.e., with passive integrated transponders) nest-guarding male Largemouth Bass in Ontario. An extremely high degree of nest site fidelity in year two was observed for males that were angled only once during year one (87% within 10 m of the previous year’s nest), 96.7% of which remained on the nest and completed parental care activities. There was significantly lower fidelity in year two, however, for males that were angled multiple times during year one (27% within 10m of the previous year’s nest), only 5.6% of which remained on the nest and completed parental care activities. This observed difference suggests that angling nesting bass may cause them to avoid previously used nest sites and instead search for alternative sites during future reproductive seasons. This human-induced impact on nest site choice may impact the future reproductive success of those Largemouth Bass.