Aaron T. Simmons

Trichomes of Lycopersicon species and their hybrids: effects on pests and natural enemies

1 The cultivated tomato, Lycopersicon esculentum, is an economically important worldwide crop. Current pest management techniques rely heavily on pesticides but trichome‐based host‐plant resistance may reduce pesticide use.

Insect attraction to synthetic herbivore-induced plant volatile-treated field crops

1 Plants produce natural enemy‐attracting semiochemicals known as herbivore‐induced plant volatiles (HIPV) in response to herbivore damage. Deployment of synthetic HIPV in crops could enhance the biological control of pests. To test this, six HIPV [methyl salicylate (MeSA), methyl anthranilate (MeA), methyl jasmonate (MeJA), benzaldehyde (Be), cis‐3‐hexenyl acetate (HA), cis‐hexen‐1‐ol (He)] in three concentrations (0.5%, 1.0% and 2.0% v/v) mixed with a vegetable oil adjuvant, Synertrol® (Organic Crop Protectants Pty Ltd, Australia), were sprayed onto winegrape, broccoli and sweet corn plants. 2 The relative abundance of insects within treated plots was assessed with non‐attracting, transparent sticky traps at varying time intervals up to 22 days after spraying. 3 In the vineyard experiment, Trichogrammatidae responded to Be and MeA (0.5%) and Be (1.0%); Encyrtidae and Bethylidae responded to MeA (1.0%); Scelionidae responded to all compounds at 1.0% and 2.0%; and predatory insects responded to MeA. In sweet corn, parasitoids as a group and Encyrtidae responded to MeA (0.5%); Braconidae responded to all compounds at 0.5% and Synertrol‐only; thrips responded to all compounds at 0.5% and 1.0%; while all parasitoids responded to all compounds at 0.5% and 1.0% and Synertrol‐only. In broccoli, parasitoids as a group and Scelionidae responded to Be, HA, He and Synertrol‐only; Trichogrammatidae responded to Be (0.5%), He (0.5% and 1.0%), MeJA (1.0%) and MeSA (0.5%); and thrips responded to all compounds at to 0.5% and 1.0%. 4 Significant attraction of insects occurred up to 6 days after the HIPV application, suggesting that plants may have been induced to produce endogenous volatiles that attracted insects over an extended period. 5 The results obtained are discussed in relation to the potential utility of synthetic HIPV to enhance the biological control of pests.

Trichome-based host plant resistance of Lycopersicon species and the biocontrol agent Mallada signata: are they compatible?

Trichome‐based host plant resistance of Lycopersicon (Solanaceae) species offers the potential to reduce pesticide use in tomato production, but its compatibility with biocontrol agents is largely unknown. The effect of trichome‐based host plant resistance on the lacewing biocontrol agent, Mallada signata, was assessed for five accessions of L. cheesmanii, four accessions of Lycopersicon hirsutum, two accessions of Lycopersicon pennellii, and one Lycopersicon esculentum cultivar. An intact leaf was isolated from the whole plant using Tangletrap to coat the petiole and 20 green peach aphids [Myzus persicae (Sulzer) (Homoptera: Aphididae)] were placed on the leaf surface. After 24 h, 10 lacewings were placed on the leaf. The numbers of dead, trapped by exudates, untrapped and predated lacewings and aphids, and the numbers that had left the leaf were recorded a further 24 h later. Differences in insect designations between accessions were analysed using ANOVA. A General Linear Model (GLM), consisting of the densities of each trichome type and leaf area, was fitted to the data to determine the role of trichomes on the observed effects on lacewings and aphids. Lacewing mortality was greater on one accession of L. pennellii and one accession of L. hirsutum than on L. esculentum. The GLM indicated that type IV trichomes decreased the numbers of aphids predated, and increased cannibalism and, along with type III trichomes, increased entrapment‐related predator mortality. Although there were no differences in the numbers of predated aphids, with the majority predated for all accessions, the compatibility of trichome‐based host plant resistance of Lycopersicon species and the biocontrol of aphids by lacewings is questionable.

Relationship between environmental and land-use variables on soil carbon levels at the regional scale in central New South Wales, Australia

The potential to change agricultural land use to increase soil carbon stocks has been proposed as a mechanism to offset greenhouse gas emissions. To estimate the potential carbon storage in the soil from regional surveys it is important to understand the influence of environmental variables (climate, soil type, and landscape) before land management can be assessed. A survey was done of 354 sites to determine soil organic carbon stock (SOC stock; Mg C/ha) across the Lachlan and Macquarie catchments of New South Wales, Australia. The influences of climate, soil physical and chemical properties, landscape position, and 10 years of land management information were assessed. The environmental variables described most of the regional variation compared with management. The strongest influence on SOC stock at 0–10 cm was from climatic variables, particularly 30-year average annual rainfall. At a soil depth of 20–30 cm, the proportion of silica (SiO2) determined by mid-infrared spectra (SiMIR) had a negative relationship with SOC stock, and sand and clay measured by particle size analysis also showed strong relationships at sites where measured. Of the difference in SOC stock explained by land use, cropping had lower soil carbon than pasture in rotation or permanent pasture at 0–10 cm. This relationship was consistent across a rainfall gradient, but once soil carbon was standardised per mm of average annual rainfall, there was a greater difference between cropping and permanent pasture with increasing SiMIR in soils. Land use is also regulated by climate, topography, and soil type, and the effect on SOC stock is better assessed in smaller land-management units to remove some variability due to climate and soil.

Trichome characteristics of F1 Lycopersicon esculentum × L. cheesmanii f. minor and L. esculentum × L. pennellii hybrids and effects on myzus persicae

SummaryThe green peach aphid (Myzus persicae) is a serious pest of the tomato (Lycopersiconesculentum). Trichome-based host plant resistance of wild Lycopersicon species may offer an alternative to pesticides for management of this pest. Two wild species, Lycopersiconcheesmanii f. minor and L. pennellii, were crossed with L. esculentum and the types and densities of trichomes possessed by the F1 hybrids measured. The effects of these trichomes on M. persicae were assessed by placing nymphs on leaves and, 96 hr later, designating each nymph as either dead, alive, emigrating off the leaf or unaccounted for. A generalised linear model was used to detect relationships between the numbers of nymphs in each designation and the densities of trichomes and leaf area. Mortality of nymphs on L. pennellii hybrids was associated with high densities of type IV and low densities of type I trichomes. Mortality of nymphs on L. cheesmanii f. minor hybrids was associated with high densities of type III trichomes. An increase in the densities of type III trichomes on L. pennellii hybrids was associated with greater numbers of nymphs emigrating off the leaf whilst unaccounted nymphs on L. pennellii hybrids were associated with increased densities of type VII and type I trichomes. Further experimentation using excised leaflets found mortality on L. pennellii hybrids may be due to nymphs being hindered by type I trichomes and increased densities of type V trichomes were associated with greater numbers of unhindered nymphs. Implications of results are discussed in the context of introducing trichome-based host plant resistance into L. esculentum.

Seedling recruitment of native perennial grasses within existing swards

Two field experiments, one each on Austrodanthonia spp. and Bothriochloa macra, investigated the effects of biomass manipulation, seed level modification, site preparation and pasture composition on the recruitment of native perennial grass seedlings. The experiments coincided with drier than average years and although successful emergence of seedlings occurred, survival was extremely low. In the Austrodanthonia experiment, control treatments resulted in the emergence of only 1 seedling/m2, whereas there were 130/m2 in the best treatment which had biomass cut with plant material removed, seed added, and the soil surface scarified. Insecticide treatments increased emergence as seed-harvesting ants are common in these systems, but the benefits were small. Similarly, B. macra had no emergence in the control treatment compared with 73 seedlings/m2 in the best treatment, which was pasture cropped, and had seed added and herbicide applied. Availability of microsites may be a major constraint to B. macra emergence, as soil disturbance through pasture cropping substantially increased seedling numbers (279/m2). The effects of herbicide on emergence were small with the largest being related to bare ground and litter biomass. Austrodanthonia seedling numbers at emergence were related to bare ground, litter and green biomass. Survival of young Austrodanthonia plants 24 weeks after emergence was negatively related to plant cover, but only in treatments where plant material was cut and removed. The success of survival was determined at 52 weeks after emergence and the number of young plants that survived in both experiments seemed to have been influenced by the presence of competitive biomass of existing plants.

Cradle-to-farmgate greenhouse gas emissions for 2-year wheat monoculture and break crop–wheat sequences in south-eastern Australia

Abstract. We used life cycle assessment methodology to determine the cradle-to-farmgate GHG emissions for rainfed wheat grown in monoculture or in sequence with the break crops canola (Brassica napus) and field peas (Pisum sativum), and for the break crops, in the south-eastern grains region of Australia. Total GHG emissions were 225 kg carbon dioxide equivalents (CO2-e)/t grain for a 3 t/ha wheat crop following wheat, compared with 199 and 172 kg CO2-e/t for wheat following canola and field peas, respectively. On an area basis, calculated emissions were 676, 677 and 586 kg CO2-e/ha for wheat following wheat, canola and field peas, respectively. Highest emissions were associated with the production and transport of fertilisers (23–28% of total GHG emissions) and their use in the field (16–23% of total GHG emissions). Production, transport and use of lime accounted for an additional 19–21% of total GHG emissions. The lower emissions for wheat after break crops were associated with higher yields, improved use of fertiliser nitrogen (N) and reduced fertiliser N inputs in the case of wheat after field peas. Emissions of GHG for the production and harvesting of canola were calculated at 841 kg CO2-e/ha, equivalent to 420 kg CO2-e/t grain. Those of field peas were 530 kg CO2-e/ha, equivalent to 294 kg CO2-e/t grain. When the gross margin returns for the crops were considered together with their GHG emissions, the field pea–wheat sequence had the highest value per unit emissions, at AU

Rubidium labelling demonstrates movement of predators from native vegetation to cotton

787/t CO2-e, followed by wheat–wheat (

Contents of Al, Cu, Fe, and Mo in Phalaris aquatica and Trifolium subterraneum grown on an unamended and amended gold-mine tailings-storage facility in central-western New South Wales, Australia

703/t CO2-e) and canola–wheat (

Attract and reward: combining chemical ecology and habitat manipulation to enhance biological control in field crops

696/t CO2-e). Uncertainties associated with emissions factor values for fertiliser N, legume-fixed N and mineralised soil organic matter N are discussed, together with the potentially high C cost of legume N2 fixation and the impact of relatively small changes in soil C during grain cropping either to offset all or most pre- and on-farm GHG emissions or to add to them.