Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Abby L. West is active.

Publication


Featured researches published by Abby L. West.


Analytical Chemistry | 2014

DNase 1 Retains Endodeoxyribonuclease Activity Following Gold Nanocluster Synthesis

Abby L. West; Mark H. Griep; Daniel P. Cole; Shashi P. Karna

Here we present the synthesis of the enzyme DNase 1 stabilized gold nanoclusters (DNase 1:AuNCs) with core size consisting of either 8 or 25 atoms. The DNase 1:Au8NCs exhibit blue fluorescence whereas the DNase 1:Au25NCs are red emitting. In addition to the intense fluorescence emission, the synthesized DNase 1:AuNC hybrid retains the native functionality of the protein, allowing simultaneous detection and digestion of DNA with a detection limit of 2 μg/mL. The DNase 1:AuNCs could be conveniently employed as efficient and fast sensors to augment the current time-consuming DNA contamination analysis techniques.


ACS Applied Materials & Interfaces | 2016

In situ Synthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells.

Abby L. West; Nicole M. Schaeublin; Mark H. Griep; Elizabeth I. Maurer-Gardner; Daniel P. Cole; Alexis M. Fakner; Saber M. Hussain; Shashi P. Karna

To date, the directed in situ synthesis of fluorescent gold nanoclusters (AuNCs) has only been demonstrated in cancerous cells, with the theorized synthesis mechanism prohibiting AuNC formation in nontumorigenic cell lines. This limitation hinders potential biostabilized AuNC-based technology in healthy cells involving both chemical and mechanical analysis, such as the direct sensing of protein function and the elucidation of local mechanical environments. Thus, new synthesis strategies are required to expand the application space of AuNCs beyond cancer-focused cellular studies. In this contribution, we have developed the methodology and demonstrated the direct in situ synthesis of AuNCs in the nontumorigenic neuronal microglial line, C8B4. The as-synthesized AuNCs form in situ and are stabilized by cellular proteins. The clusters exhibit bright green fluorescence and demonstrate low (<10%) toxicity. Interestingly, elevated ROS levels were not required for the in situ formation of AuNCs, although intracellular reductants such as glutamate were required for the synthesis of AuNCs in C8B4 cells. To our knowledge, this is the first-ever demonstration of AuNC synthesis in nontumorigenic cells and, as such, it considerably expands the application space of biostabilized fluorescent AuNCs.


IEEE Nanotechnology Magazine | 2015

Gold Nanocluster DNase 1 Hybrid Materials: An Efficient Method for DNA Contamination Sensing.

Abby L. West; Mark H. Griep; Dan P. Cole; Shashi P. Karna

Protein-encapsulated gold nanocluster (P-AuNC) synthesis was first demonstrated in 2009 [1]. Initially, these P-AuNCs were used as cellular imaging agents as the protein shell surrounding the AuNC made them highly biocompatible. However, recent studies have begun to show that these stabilizing proteins may also retain native biological function, thus giving a dual functionality to these hybrid molecules. Here, we present the synthesis of DNase 1 stabilized AuNCs (DNase 1:AuNCs) with core sizes consisting of either eight or 25 atoms. The DNase 1:Au8NCs exhibit blue fluorescence, whereas the DNase 1:Au25NCs are red emitting. Moreover, in addition to the intense fluorescence emission, the synthesized DNase 1:AuNC hybrids retain the native functionality of the protein, allowing simultaneous detection and digestion of DNA with a detection limit of 2 mg/mL (Figure 1). The DNase 1:AuNCs could be conveniently employed as efficient and fast sensors to augment the current inefficient and time-consuming DNA contamination analysis techniques.


international conference on nanotechnology | 2014

Gold nanocluster-DNase 1 hybrid materials for DNA contamination sensing

Abby L. West; Mark H. Griep; Dan P. Cole; Shashi P. Karna

Protein encapsulated gold nanocluster (P-AuNC) synthesis was first demonstrated in 2009.[1] Initially these P-AuNCs were used as cellular imaging agents as the protein shell surrounding the AuNC made them highly biocompatible. However, recent studies have begun to show that these stabilizing proteins may also retain native biological function thus giving a dual functionality to these hybrid molecules. Here we present the synthesis of DNase 1 stabilized gold nanoclusters (DNase 1:AuNCs) with core sizes consisting either 8 or 25 atoms. The DNase 1:Au8NCs exhibit blue fluorescence whereas the DNase 1:Au25NCs are red emitting. Moreover, in addition to the intense fluorescence emission; the synthesized DNase 1:AuNC hybrid retain the native functionality of the protein, allowing simultaneous detection and digestion of DNA with a detection limit of 2 μg/mL (Scheme 1). The DNase 1:AuNCs could be conveniently employed as efficient and fast sensors to augment the current inefficient and time consuming DNA contamination analysis techniques.


Archive | 2018

Site-Specific Nanocluster Synthesis in Energy-Coupled Biomolecular Hosts

Sasha Teymorian; Abby L. West; Michael Lee; Nick Bedford; Mark H. Griep


Archive | 2016

Purification Efficacy of Synthetic Cannabinoid Conjugates Using High-Pressure Liquid Chromatography

Alexis M. Fakner; Abby L. West; Shashi P. Karna; Mark H. Griep


Archive | 2016

Stabilizing Protein Effects on the Pressure Sensitivity of Fluorescent Gold Nanoclusters

Abby L. West; Mark H. Griep; Christopher Knoblauch; Shashi P. Karna


Archive | 2016

Use of an Ethanol-Driven Pressure Cell to Measure Hydrostatic Pressure Response of Protein-Stabilized Gold Nanoclusters

Abby L. West; Mark H. Griep; Christopher Knoblauch; Shashi P. Karna


Archive | 2016

Fluorescent Pressure Response of Protein-Nanocluster Polymer Composites

Alin Cristian. Chipara; Mark H. Griep; Timothy Walter; Abby L. West


Archive | 2015

Conjugation of the Dark Quencher QSY 7 to Various Synthetic Cannabinoids for Use in Fluorescence-Based Detection Platforms

Abby L. West; Nabila Hoque; Joseph Dougherty; Shashi P. Karna; Mark H. Griep

Collaboration


Dive into the Abby L. West's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar

Nicole M. Schaeublin

Air Force Research Laboratory

View shared research outputs
Top Co-Authors

Avatar

Saber M. Hussain

Wright-Patterson Air Force Base

View shared research outputs
Researchain Logo
Decentralizing Knowledge