Abdullah Eryavuz

The effects of dietary boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats

The aims of this study were to clarify the effects of high dietary supplementation with boric acid and borax, called boron (B) compounds, on lipid peroxidation (LPO), antioxidant activity, some vitamin levels, and DNA damage in rats. Thirty Sprague Dawley male rats were divided into three equal groups: the animals in the first group (control) were fed with a standard rodent diet containing 6.4 mg B/kg, and the animals in the experimental group were fed with a standard rodent diet added with a supra-nutritional amount of boric acid and borax (100 mg B/kg) throughout the experimental period of 28 days. The B compounds decreased malondialdehyde (MDA), DNA damage, the protein carbonyl content (PCO) level in blood, and glutathione (GSH) concentration in the liver, Cu-Zn superoxide dismutase (SOD), and catalase (CAT) activity in the kidney. The B compounds increased GSH concentration in blood and the vitamin C level in plasma. Consequently, our results demonstrate that B supplementation (100 mg/kg) in diet decreases LPO, and enhances the antioxidant defense mechanism and vitamin status. There are no differences in oxidant/antioxidant balance and biochemical parameters except for serum vitamin A and liver GSH concentration, between the boron compounds used in this study.

Effects of urea and sulfur on performance of faunate and defaunate Ramlic lambs, and some rumen and blood parameters

This study was conducted to investigate effects of defaunation, and addition of urea and sulfur, in replacement of plant protein (PP) sources on levels of some blood and rumen parameters, and body weight (BW) gain in lambs. Forty male ramlic lambs, 2.70 ± 0.30 months of age, and weighing approximately 16.44 ± 0.41 kg were used. Lambs were divided into four groups as: faunate + control diet (FC); defaunate + control diet (DC); faunate + experimental diet (FE); and defaunate + experimental diet (DE). The control diet contained plant protein as a N source, whereas the experimental diet was supplemented with urea and sulfur, both at 3% of BW per day. The forage portion of the diet was 350 g of alfalfa hay per lamb per day. Diets were fed twice daily in equal amounts. Defaunation, or feeding a diet supplemented with urea and sulfur in place of plant protein sources, had no effect on BW gain. Ruminal pH and ammonia N concentrations were higher ( P< 0.001) with the experimental diet versus the control diet, while they were not affected by defaunation. Nitrate concentrations in rumen fluid were decreased by defaunation on days 30 ( P< 0.001) and 60 (P = 0.04), but not affected with the experimental diet. There was a main effect interaction for the nitrite concentration in the rumen fluid on days 60 ( P< 0.001) and 90 (P = 0.02). Protozoa disappeared from the rumen of lambs fed the experimental diet, whereas protozoa in the rumen contents of lambs fed the control diet were highest on day 30, but decreased thereafter. Plasma glucose and urea N levels were higher ( P< 0.001) in lambs fed the experimental diet. On day 30, there was a main effect interaction on blood methemoglobin levels. Methemoglobin Abbreviations: AA, amino acid; BW, body weight; DC, defaunate lambs fed the control diet; DE, defaunate lambs fed the experimental diet; DSS, dioctylesodium sulphosuccinate; FC, faunate lamb fed the control diet; FD × PN, faunate and defaunate versus plant protein and non-protein nitrogen; FD, faunate versus defaunate; FE, faunate lamb fed the experimental diet; NPN, non-protein nitrogen; PP, plant protein; PN, plant protein versus non-protein nitrogen; PUN, plasma urea nitrogen; SEM, standard error of the mean

Effects of Yucca Schidigera To Diet of Dairy Cows on Blood Oxidant-Antioxidant Balance, Biochemical Parameters, and Milk Quality

Hayvan beslemede verim artirici ve buyumeyi tesvik edici olarak kullanilan ionofor grubu antibiyotikler basta olmak uzere bircok kimyasal katki maddesi Avrupa’da yasaklanmistir. Son yillarda bu maddelerin yerine bitkilerden elde edilen saponinler gibi biyolojik aktif urunler kullanilmaktadir. Bu arastirmanin amaci steroidal saponin iceren Yucca schidigera (YS) bitki tozunun sutcu sigirlarda kan oksidan-antioksidan denge ve sutte malondialdehit, ure-N ile sut kalitesi uzerine etkilerini belirlemektir. Calismada ortalama sut verimi 20,7 kg ± 3,1 kg/gun ve canli agirliklari 552 kg ± 30 kg olan 10 adet Holstayn inek kullanildi. Calismanin baslangicinda ve 15 gunun sonunda (15 gun boyunca rasyonlarina 1500 pp Yucca schidigera ilave edildi) bu hayvanlardan sabah yemlemesinden oncesi ve 2 saat sonrasinda kan ve sut ornekleri alindi. Kanda malondialdehit (MDA), antioksidan aktivite (AOA), redukte glutatyon (GSH), superoksid dismutaz (SOD), katalaz (CAT), A vitamini, beta karoten, amonyak tayini ve sut orneklerinde MDA, ure-N ile sut kalite parametreleri olculdu. Istatistik analizler icin SPSS paket programi, verilerin degerlendirilmesinde paired-t testi uygulandi. Yemleme oncesi ve sonrasinda kanda MDA ve amonyak duzeyleri dusuk bulundu (p < 0,05), yemleme oncesi GSH yemleme sonrasi GSH ve β-karoten duzeyleri ise yuksek bulundu (p < 0,05). Calismada yeme YS ilavesinin sut MDA ve ure azotu duzeylerini dusurdugu (p ˂ 0.05), sut yag yuzdesini artirdigi (p ˂ 0.05), diger parametrelere ise etkisinin olmadigi bulundu. Bu bulgular YS ilavesinin sutte MDA ve ure azotu duzeylerini dusurdugunu gosteren ilk calismadir. Sonuc olarak, bu calismada elde edilen bulgular, sut ineklerinin rasyonlarina dogal bir antioksidan olarak YS ilavesinin kanda antioksidan savunmayi guclendirebilecegi ve sut kalitesinin artmasinda olumlu bir etkisinin olabilecegini gostermektedir.