Abdullah Yusuf

In vitro anti-arthritic and thrombolytic activities of methanolic extract of Protium serratum leaves

The present study was engineered to find out the anti-arthritic and thrombolytic activity of methanolic extract of Protium serratum by using in vitro method. Methanolic extract of P. serratum was assessed with the inhibition of protein denaturation model which was used to evaluate anti-arthritic potential and this extract assessed with human blood to evaluate thrombolytic effect. The extract showed remarkable anti-arthritic activity which was evaluated by the percentages of inhibition of protein denaturation in different concentration of plant extract compared to diclofenac sodium, a reference drug. The maximum percentage inhibition of protein denaturation was observed as 83.94% at 1000 μg/ml. It has significant thrombolytic effect compared to streptokinase. During assay for thrombolytic activity, the methanolic extract of P. serratum revealed 59.653 ± 8.626% lysis of clot, while standard streptokinase (SK) and water used as positive and negative controls, demonstrated 72.835 ± 5.702 and 2.725 ± 0.983% lysis of clot, respectively. These findings demonstrate that the leaves extract of P. serratum have excellent antiarthritic and thrombolytic effect.

Complications of Therapeutic Plasma Exchange in Patient with Neurological Disorders

Background: Therapeutic plasma exchange (TPE) is a procedure used in neurological disorders where autoimmunity plays a major role. Though it is a relatively safe procedure, severe complications may occur.Objective: The aim of the present study was to analyze the incidence of TPE related complications in patients with different neurological disorders.Methodology: This prospective study was conducted in 91 patients, in department of Transfusion Medicine at National Institute of Neurosciences and Hospital (NINS) from February 2014 to January 2017 for a period of three (03) years. All admitted patients with neurological disorders advised for TPE were included in this study.Results: Age range was from 13 to 70 years with a mean age of 37.14±13.79 years. 67(74%) were men and 24(26.0%) were women. The most common diagnosis was Guillain-Barre Syndrome (GBS) in 79.1 % (n=72) cases followed by myasthenia gravis (MG) in 13.2% (n=12), chronic inflammatory demyelinating polyneuropathy (CIDP) in 6.6 % (n= 6), multifocal motor neuropathy (MMN) in 1.1% (n=1) cases. Total number of 332 TPE sessions was performed with the median of 4 sessions per patient (range 1 to 9). Total 99 (29.8%) adverse reactions occurred in 332 TPE sessions. The most common adverse effect was hypotension (n=35, 10.54 %) followed by allergic reactions (n=29, 8.73 %), access problem (n= 12, 3.61 %) and vomiting (n=8, 2.41 %). Most of the adverse reactions were mild and improved spontaneously or by using simple medications. Out of 332 TPE sessions, 330(99.4%) procedures were completed successfully after managing the adverse reactions. TPE were discontinued in 2 occasions (0.6% of procedures) due to inadequate venous access. There was no mortality related to TPE procedure.Conclusion: In spite of few complications, TPE can be considered as a safe method of treatment in neurologic disorders when carried out with all necessary precautions.Journal of National Institute of Neurosciences Bangladesh, 2017;3(2): 69-74