Abel Silveira Cardoso

Heterogeneity of metallo and serine extracellular proteinases in oral clinical isolates of Candida albicans in HIV-positive and healthy children from Rio de Janeiro, Brazil

Candida yeasts frequently cause life-threatening systemic infections in immunocompromised hosts. In the present study, gelatin-SDS-PAGE analysis was used to characterize extracellular proteinases in 44 oral clinical isolates of Candida albicans from HIV-positive (29/50) and healthy children (15/50). Our survey indicates that these oral clinical isolates of C. albicans have complex extracellular proteolytic activity profiles, which illustrates the heterogeneity of this species. We showed four distinct proteolytic patterns composed of distinct serine (30-58 kDa) and metalloproteinase (64-95 kDa) activities, based on the inhibition profile with phenylmethylsulfonyl fluoride and 1,10-phenanthroline, respectively. This is the first report on secreted serine and metalloproteinases present in the culture supernatant fluids of C. albicans; however, we did not observe a significant correlation between proteolytic profile expressed by the C. albicans isolates from HIV-positive children and CD4(+) T cell count and plasma viral load.

Metastatic renal cell carcinoma to the oral cavity and clear cell mucoepidermoid carcinoma: comparative clinicopathologic and immunohistochemical study.

BACKGROUND Metastatic clear cell renal cell carcinoma (CCRCC) should be considered in differential diagnosis of intraoral clear cell tumors, including mucoepidermoid carcinoma (MEC). OBJECTIVE AND STUDY DESIGN We compared the clinical, histologic, histochemical, and immunohistochemical characteristics of 9 oral metastatic CCRCCs and 8 intraoral clear cell MECs. RESULTS Oral metastatic CCRCC affected salivary-gland containing tissues in 7 cases (78%). Microscopically, oral metastasis revealed a proliferation of neoplastic clear cells arranged in an alveolar pattern with central blood vessels, features that were not seen in any intraoral clear cell MEC. Mucicarmine staining was positive only in clear cell MEC. Immunohistochemistry showed similarities in cytokeratin expression; vimentin and CD10 were expressed in all oral metastatic CCRCCs but in only 1 clear cell MEC each. CONCLUSIONS Besides clinical history, the alveolar pattern, vessel distribution, absence of mucicarmine staining, and vimentin and CD10 immunoexpression are useful in histologic differential diagnosis of CCRCC and clear cell MEC.

Carcinoma de células escamosas da cavidade oral em pacientes jovens e sua crescente incidência: revisão de literatura

The purpose of this study was to review the squamous cell carcinoma of the oral cavity in young population. The literature shows a different behavior in this type of disease; it seems to be more aggressive. There is a close relationship between some habits (tobacco and alcohol consumption) and the development of squamous cell carcinoma of the oral cavity, but in this case the patients normally relate no risk factors. There is a little number of case report of squamous cell carcinoma of the oral cavity in patients under 40 years old, so its difficult to prove the increasing of the squamous cell carcinoma of the oral cavity as said in the literature. Further studies are necessary to know better this entity. The identification of the characteristics of this young population is necessary, because it might reflect problems in cancer control and can enable the development of a primary prevention program for squamous cell carcinoma of the oral cavity in young patients.

Blue nevus of the oral mucosa: Review of the literature and report of two cases

Abstract Intraoral blue nevus has been infrequently reported. A review of the literature yielded twenty-two cases. A clinicopathologic study of the previous twenty-two cases and our two cases suggests that this lesion has no age or sex predilection. The most common site of occurrence was the hard palate. There appears to be no tendency for recurrence.

Surgical Treatment of Stage III Bilateral Maxillary Osteonecrosis in an Oncologic Patient

MAXILLARY OSTEONECROSIS IN AN ONCOLOGIC PATIENT. ANA CAROLINA ORGANISTA CORNER, ABEL SILVEIRA CARDOSO, HERNANDO VALENTIM DA ROCHA JÚNIOR, NICOLAS HOMSI, EDUARDO SEIXAS CARDOSO. Bisphosphonate-induced maxillary osteonecrosis is characterized by an exposed bone area in the jaws. The bisphosphonates are widely used in the treatment of osteoporosis and other diseases that may cause bone loss. The disease is classified in 3 stages, with the third, the most severe, characterized by necrotic bone exposure beyond the alveolar ridge region and observed on x-ray and computed tomography exams. This report described a stage III clinical case of bilateral maxillary osteonecrosis. A buccal fat pad was used for bucosinusal fistulae closure and occlusion of the bone defect after resection of the meso and infrastructure of the upper jaw in the surgical treatment of a large area of jaw osteonecrosis after teeth extractions in a patient using aminobisphosphonate to control the progression of bone metastasis of adenocarcinoma of the prostate. The follow-up continued for 5 years, and the patient died due to the primary disease.