Adam Deutsch

Changes in the deoxyadenylate regions in 5 cases of chronic lymphocytic leukemic DNA

Abstract The (dA) levels in the DNAs from normal human tissues and from the lymphocytes of five CLL patients were estimated by formation of ribonuclease A-resistant poly (U)-DNA complexes. Alterations in the (dA) levels of all five CLL DNAs were observed when comparison was made to the normal (dA) pattern. The type of (dA) alterations varied among the different CLL patients, but the variation correlated to the WBC levels of the patients at the time of study. A specificity of neoplastic-associated (dA) alterations for slow repeated + unique DNA sequences and for main band DNA was demonstrated with two of the CLL DNAs.

Alterations in the deoxyadenylate regions of the DNA from four human breast tumors.

The levels of (dA) regions in 4 breast tumor DNAs were compared with the level in normal DNA. Although the (dA) regions in normal DNA remained constant in breast tissue, lymphocytes and placenta, (dA) alterations from the normal pattern were observed in the DNA from all four breast tumors. Tumor histology varied and was parallelled by a corresponding variation in the (dA) alterations following a quantitative analysis of the (dA) rich and (dA) “pure” sequences. The (dA) alterations associated with 2 of the tumor DNAs were distributed into both A + T rich and G + C rich DNA fractions.