Ademilso Sampaio de Oliveira

Cytogenetic and identification of the nucleolus organizer region in Heliconia bihai (L.) L.

The genus Heliconia is not much studied and the number of existing species in this genus is still uncertain. It is known that this number relies between 150 to 250 species. In Brazil, about 40 species are native and known by many different names. The objective of this paper was to characterize morphometrically and to identify the NOR (active nucleolus organizer regions) by Ag-NOR banding of chromosomes of Heliconia bihai (L) L. Root meristems were submitted to blocking treatment in an amiprofos-methyl (APM) solution, fixed in methanol-acetic acid solution for 24 hours, at least. The meristems were washed in distilled water and submitted to enzymatic digestion with pectinase enzyme. The slides were prepared by dissociation of the root meristem, dried in the air and also on hot plate at 50°C. Subsequently, some slides were submitted to 5% Giemsa stain for karyotype construction and to a solution of silver nitrate (AgNO 3 ) 50% for Ag-NOR banding. The species H. bihai has 2n = 22 chromosomes, 4 pairs of submetacentric chromosomes and 7 pairs of metacentric chromosomes, and graded medium to short (3.96 to 0.67 μM), with the presence of active NOR in pairs 1 and 2 and interphase cells with 2 nucleoli. These are the features of a diploid species.

CONFLITOS DE USO E OCUPACAO DOS SOLOS NO ASSENTAMENTO RURAL SAO PAULO NO MUNICIPIO DE CARLINDA, MT

The objective of this work was to map the land use and occupation in a rural settlement with an area of 2,413,9015 ha, located in the municipality of Carlinda MT, in the northern region of the state. To diagnose conflicts, according to the new Forest Code, Law no. 12.651, of May 25, 2012. For the mapping of the ground cover a supervised classification was used, realized in software ArcGIS 10.1 by means of orbital images of the satellites SPOT5 of the year 2008 and OLI-Landsat-8 of the years 2013, 2015. The results obtained showed that the APPs in 2008 were with