Adrian P. Rowley

Elastic properties of porcine ocular posterior soft tissues

We examine the thickness and mechanical properties of the porcine posterior retina, choroid, and sclera in different environments and surface directions. Vertical and horizontal samples were surgically obtained. Uniaxial experiments were performed in room-temperature air, room-temperature saline, and body-temperature saline. Sample thicknesses were estimated optically. Thickness of all layers was found to vary significantly among the samples; thickness standard deviation of the mean was 24, 19, and 19% for the retina, choroid, and sclera, respectively. Transition stresses and heel moduli of all layers were consistently higher in saline than air. The retinal stress-strain relationship in air was typically linear with significantly lower horizontal transition strain. Transition stresses and moduli of all layers were consistently lower in body than room temperature and the differences in the transition stresses and heel moduli of the retina and sclera were significant. Also, the sclera had significantly lower transition strains in body temperature. These results illustrate the importance of testing the tissues at conditions like those found in the body. In body-temperature saline, all layers behaved nonlinearly, but only the retina exhibited surface anisotropy between the vertical and horizontal directions.

Finite element modeling of retinal prosthesis mechanics.

Epiretinal prostheses used to treat degenerative retina diseases apply stimulus via an electrode array fixed to the ganglion cell side of the retina. Mechanical pressure applied by these arrays to the retina, both during initial insertion and throughout chronic use, could cause sufficient retinal damage to reduce the devices effectiveness. In order to understand and minimize potential mechanical damage, we have used finite element analysis to model mechanical interactions between an electrode array and the retina in both acute and chronic loading configurations. Modeling indicates that an acute tacking force distributes stress primarily underneath the tack site and heel edge of the array, while more moderate chronic stresses are distributed more evenly underneath the array. Retinal damage in a canine model chronically implanted with a similar array occurred in correlating locations, and model predictions correlate well with benchtop eyewall compression tests. This model provides retinal prosthesis researchers with a tool to optimize the mechanical electrode array design, but the techniques used here represent a unique effort to combine a modifiable device and soft biological tissues in the same model and those techniques could be extended to other devices that come into mechanical contact with soft neural tissues.

Novel method to quantify traction in a vitrectomy procedure

Aim To report a novel method to quantify traction applied to the retina using vitreous cutters during pars plana vitrectomy. Methods Fresh porcine eyes were positioned in a specially developed holder and transfixed to the retinal layers with a wire and the other end fixed to the load cell of a strain gauge. Five separate 20-gauge electrical drive mechanism vitrectors were introduced into the eye at a 45° angle and positioned at a distance of either 3 or 5 mm from the retina. Data from the strain gauge were acquired and the traction force computed. Results The analysis revealed that the vitreoretinal traction increased by 7.90 dynes for each 100 mm Hg increase in vacuum (p<0.05). The traction forces decreased by 2.51 dynes for each 500 cuts per minute increased (p<0.05) and the traction force increased by 2.17 dynes at 3 mm compared with 5 mm (p<0.05). Conclusion The traction was directly proportional to the aspiration vacuum and inversely proportional to the cut rate. The cutter traction force increased with proximity to the retina.