Adriana Pittella Sudré

Lagochilascariasis in a housecat and the potential risk for human disease

Barbosa et al.10 demonstrated that the domestic cat has a pattern of infection by L. minor very similar to those of humans, thus acting as reservoirs for this parasite. We report here the first case of L. minor natural infection in a domestic cat in the state of Rio de Janeiro, Brazil. Parasitism was not detected in the owner, but his close relationship with the cat increases the potential risk for human infection, bringing up the concern of new human cases in this area, making this information valuable for development of public health measures. Dear editor, Human lagochilascariasis is a rare zoonosis characterized by subcutaneous purulent lesions caused by Lagochilascaris sp. (Nematoda, Ascaridida), parasites of wild cats. The parasite natural life cycle and mechanisms of infection are poorly known. Definitive host infection occurs by preying on intermediate hosts with encysted L3 larvae in their muscle tissue.1 There are five known species of the genera Lagochilascaris, but only Lagochilascaris minor was associated with human infection. Currently, L. minor and Lagochilascaris major have been found parasitizing cats in Brazil,2,3 and only L. minor was reported to infect humans.4 In Brazil, L. major was found naturally infecting two domestic cats,3 and L. minor was recorded in one domestic cat.2 We report the second case of natural infection by L. minor in domestic cats (Felis catus) in Brazil, the first in Rio de Janeiro state, calling attention for potential human infection. A 2-year old female mixed-breed cat, weighting 3 kg, living in a farm situated in Km 52 of Rio-Friburgo Road in the municipality of Cachoeiras de Macacu (22° 27’49”S, 42° 39’09”W), Rio de Janeiro state, Brazil, presented anorexia, prostration and weight loss. The animal had an abscess in the right side of the neck ventral region with intense itching. During the drainage of the abscess a bloody secretion with 11 milky-white color helminthes were found and collected for identification. The helminthes were fixed in hot AFA (alcohol 70o GL, 93 mL; formaldehyde, 5 mL; acetic acid, 2 mL), clarified with acetic acid and phenol, mounted in slides with Canada balsam and deposited in the Helminthological Collection of the Instituto Oswaldo Cruz (CHIOC), number 35752 (whole mounts). Adult parasites had 14 to 21 mm length by 0.45 to 0.61 mm width and were identified as L. minor.5,6 Microphotographs were obtained with Olympus BX41 bright field microscope (Fig. 1). The first Brazilian case of human lagochilascariasis was described by Artigas et al.7 in the state of São Paulo, and today Brazil has the highest number (88) of human cases reported in the literature.4 However, lagochilascariasis in naturally infected domestic cats has been rarely reported, with only a few cases in Uruguay,5,8 Argentina,9 and Brazil.2,3 Letter to the Editor

TOXOPLASMOSIS-RELATED KNOWLEDGE AMONG PREGNANT AND POSTPARTUM WOMEN ATTENDED IN PUBLIC HEALTH UNITS IN NITERÓI, RIO DE JANEIRO, BRAZIL

The present study conducted a toxoplasmosis-related knowledge level survey with 400 pregnant and puerperal women attended in public health units in the municipality of Niterói, Rio de Janeiro. Only 111 (27.8%) women claimed to know about the disease. Most of them (n = 289; 72.2%) had never heard about toxoplasmosis nor knew how to prevent the infection by Toxoplasma gondii. A significant difference (p = 0.013) regarding the presence of anti-T. gondii IgG was observed between women who claimed to know about the disease and those who had never heard about it. These results highlight the importance of a systematic serological screening process for toxoplasmosis, as well as the importance of primary prevention by accurate information during prenatal care, an important Public Health action to be implemented.

Ectopic dirofilariosis in two dogs from Rio de Janeiro state, Brazil

Report of two canine dirofilariosis cases of ectopic location in the state of Rio de Janeiro. This is the first report of erratic migration for this parasitosis in dogs in the state, calling attention to the short period of time between the two cases. The fact that the area is endemic for this parasite, its zoonotic potential and the report of human cases in the state, demonstrates that authorities should be alerted to the control programs of dirofilariosis along with the pathogenic profile of the infections.

Methodological innovations for the study of irreplaceable samples reveal giardiasis in extinct animals (Nothrotherium maquinense and Palaeolama maior)

The use of diagnostic methods that prevent irreplaceable samples (from museum collections, archaeological and paleontological samples) of being consumed or that increase their yield is relevant. For museum collections, archaeological and paleontological samples it is essential to conserve samples, subsamples or portions for future research. We are addressing methods for conservation of irreplaceable samples that could be fully consumed. Innovations in methodologies that are used in studies of Paleoparasitology and Paleomicrobiology will contribute to the preservation of collections. Therefore, to the development of archaeology and paleontology in the future, we evaluated whether the discarded material of the immunochromatography test could be used for molecular diagnosis and vice versa. We used a genotyped experimental coprolite positive for Giardia duodenalis. The diagnosis was positive for giardiasis in both cases. This methodology can be corroborated with the coprolite of a Paleolama maior (extinct llama) previously diagnosed for G. duodenalis with an immunoenzymatic test. The residue of the pre-digestion step of the DNA extraction before adding Proteinase K was confirmed positive with the immunochromatographic test. Also, the DNA extraction residue from a coprolite of Nothrotherium maquinense (ground sloth) was tested positive with immunochromatographic test for G. duodenalis. These are the oldest findings for G. duodenalis confirming that this intestinal parasite occurred among Northeastern Brazilian Megafauna animals from the late Pleistocene period, correlated to human occupation. The relevance of these results will allow the study by different methodological approaches from a small amount of material, reusing discarded materials.

Assessment of the diagnostic performance of four methods for the detection of Giardia duodenalis in fecal samples from human, canine and feline carriers

Enteric parasitic diseases including giardiasis are of public health concern. Different methods are available for the diagnosis of this parasitic infection in fecal samples such as the identification of protozoan cysts and trophozoites by light microscopy, detection of specific antigens by ELISA, and amplification of DNA fragments by PCR. The present study aimed at assessing the performance of four laboratory tests for the detection of Giardia duodenalis in fecal specimens from three different host species with a previous diagnosis of giardiasis; canine, feline and human patients provided new stool samples to be retested for Giardia before initiating treatment with antiprotozoal drugs. For this purpose, triplicate fecal specimens from 54 humans, 24 dogs and 18 cats living in the city of Niterói, RJ, southeast Brazil, were analysed by light microscopy, ELISA, immunochromatography, and nested PCR. The centrifugal-flotation method detected Giardia cysts in 89.6% (86/96) of the fecal samples. The protozoan parasite was detected via immunochromatography in 87.5% (84/96) of these samples. Giardia was detected by ELISA in 69.8% (67/96) of the stool specimens from carriers with a previous diagnosis of Giardia infection. Giardia was detected by PCR in only 39.6% (38/96) of the fecal specimens. Based on these findings, we suggest that, among the four assays that were used in this study, the zinc sulphate flotation technique (Faust et al., 1939) is the best diagnostic assay in terms of sensitivity and specificity to detect G. duodenalis on serially collected samples from dogs, cats and humans.

Modification of the Alere GIARDIA Ag TEST immunochromatography KIT methodology for its use in frozen fecal sediment of dogs and cats

Giardia duodenalis is a worldwide intestinal parasite and is one of the most frequent protozoa species infecting dogs and cats. This study aimed to modify the methodology of Alere GIARDIA Ag TEST KIT for its use in frozen fecal sediments with different storage times in a freezer (-20°C), thus expanding the range of use of this methodology. One hundred fecal sediments from dogs (n=50) and cats (n=50) previously examined by optical microscopy for Giardia cysts were selected for this study. The agreement between the modified immunochromatography and microscopy results was calculated by Kappa coefficient. To evaluate the performance of the modified immunochromatography assay on samples with different storage time, the fecal sediments were divided into three groups according to the time of storage in a freezer: (a) ≤ 1 year (n=37); (b) > 1 year and ≤ 3 years (n=39); (c) > 10 years (max. 13 years) (n=24). The results obtained by the modified immunochromatography assay demonstrates a higher sensitivity of this technique when compared with microscopy, regardless of the frozen storage time. These results allow for the use of this methodology in a greater scope of analysis, especially in frozen fecal sediment triage in sample collections, enabling epidemiological and comparative analysis along different decades.

Captive-bred neotropical birds diagnosed with Cryptosporidium Avian genotype III

Currently, there are only three valid species of Cryptosporidium infecting avian hosts, namely, Cryptosporidium meleagridis, Cryptosporidium baileyi, Cryptosporidium galli and Cryptosporidium avium in addition to 12 genotypes of unknown species status. The objectives of this study were to microscopically diagnose the presence of Cryptosporidium in birds from a commercial aviary located in Rio de Janeiro, Brazil; genotypically characterize species and/or genotypes of genus Cryptosporidum; and conduct sequencing and phylogenetic analyses to compare the obtained DNA sequences with those deposited in GenBank. A total of 85 fecal samples were collected from wild captive-bred birds: 48 of family Psittacidae and 37 of family Ramphastidae. Initially, a search for the presence of Cryptosporidium sp. oocysts was conducted using the centrifugal-flotation in saturated sugar solution technique, after that, the collected samples were analyzed microscopically. Cryptosporidium infections were only detected in 24.32% of samples belonging to the family Ramphastidae. DNA was extracted from positive samples and molecular diagnostics was applied targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis. The Cryptosporidium Avian genotype III was diagnosed in this study more closely related to the gastric species. This is the first record of Cryptosporidium Avian genotype III in order Piciformes and family Ramphastidae, where three host species (Ramphastus toco, Ramphastus tucanus, and Pteroglossus bailloni) were positive for the etiologic agent. Based on the molecular data obtained, these wild birds raised in captivity do not represent a source of human cryptosporidiosis, considering that Cryptosporidium Avian genotype III does not constitute a zoonosis.