Adriano A. Bordalo

Macrobenthic community in the Douro estuary: relations with trace metals and natural sediment characteristics.

The relationship between macrobenthic community structure and natural characteristics of sediment and trace metal contamination were studied in the lower Douro estuary (Portugal, NW, Iberian Peninsula), using an innovative threefold approach (SQG, Sediment Quality Guidelines), metal normalization to Fe, and macrobenthic community structure. This study allowed detection of a clear signature of anthropogenic contamination, in terms of Zn, Cu, Pb, and Cr in the north bank of the estuary, which experiences high urban pressure. Using the SQG approach, metal concentrations above ERM (effects range-median) were observed only at one sampling station, but several stations had levels above ERL (effects range-low). The macrobenthic community had a low diversity, with only 19 species found in the entire estuarine area, dominated by opportunistic species. The granulometric distribution of the sediments (estimated from the combination of organic matter, Fe and Al) seemed to be the major structuring factor for the communities, establishing the natural macrobenthic distribution pattern. The metals (Zn, Cu, Pb, and Cr) seemed to act as a disturbing factor over the natural distribution, with deleterious consequences for the macrobenthic communities.

Water quality and uses of the Bangpakong River (eastern Thailand).

The Bangpakong River is the most important watershed in the Eastern part of Thailand. Water quality parameters were sampled from June 1998 through May 1999 at 11 sites along a 227 km gradient, covering the wet season (June-November) and the dry season (December-May). Surface water was collected at three different stations per site (close to the banks and in the middle of the river), and analyzed for temperature, dissolved oxygen, turbidity, suspended solids, pH. ammonia, fecal coliforms, biochemical oxygen demand and chemical oxygen demand as well as conductivity, phosphate, and heavy metals. The Scottish water quality index (WQI) was adaptated to the tropical environment. The averaged WQI was low (41%) and quality declined significantly during the dry season (ANOVA, p<0.001). Although the quality rose somewhat at middle sites, only 27% of the WQI values during wet season and 2.5% during dry season were higher than 50%, denoting poor environmental quality. Within each season, the main sources of variability were the differences between sites along the gradient (48% during the wet season, 63% during the dry season), whereas monthly variability represented less than 20% of the variability. The seasonal results show that the river is suitable only for tolerant fish and wildlife species and is of doubtful use for potable water supply during the dry season. As quality improves during the wet period, water can be used for the production of potable water, but only with advanced treatment, and for indirect and noncontact recreational activities. In the middle stretches of the river, higher water quality permits multiple uses at moderate cost.

The Douro estuary (Portugal) : a mesotidal salt wedge

Abstract The Douro River drains a large part of the Iberian Peninsula before it flows into the Atlantic Ocean in northern Portugal. Although heavily dammed throughout its course, the Douro forms a mesotidal estuary in the last 22 km. No data had ever been collected in this estuary prior to 1994. An analysis of salinity data measured once a month in 1994 and current data obtained in the fall of 1994 revealed a salt wedge estuary highly dependent on river discharge: strong vertical salinity stratification under conditions of low runoff, whereas during high river flows the whole estuary became a river. The tide in the fall of 1994 was very close to a standing wave throughout the estuary; the tidal range, as high as 2.8 m at spring tides, was not powerful enough to destroy the vertical stratification. Tidal straining prevented mixing within the salt wedge during ebb; however, the column became vertically mixed at the end of flood. The salt wedge became arrested at three favored positions determined by the bathymetry. The salt intrusion, more sensitive to freshwater inflow than to tidal action, penetrated as far as 2/3 of the estuarys length. Residence times were estimated with a box model and were dependent on river discharge; they varied from 8 hours with early spring (March) inflows to 16 days under summer runoff conditions.

Survival of faecal indicator bacteria in tropical estuarine waters (Bangpakong River, Thailand)

Aims: To investigate the survival of cultivable bacteria in the tropical Bangpakong estuary (Eastern Thailand) under different salinities and light conditions.

Can PAHs influence Cu accumulation by salt marsh plants

The presence of polycyclic aromatic hydrocarbons (PAHs) may change the mechanisms of metal uptake, thus influencing kinetics and extent of metal phytoextraction. Studies on the subject are scarce, particularly for salt marsh plants. The aim of this work was to investigate the effect of PAHs on the uptake of Cu by Halimione portulacoides, a plant commonly found in salt marshes. Experiments were carried out in the laboratory, either in hydroponics (sediment elutriate) or in sediment soaked in elutriate, which were prepared with sediment and water from a salt marsh of the Cavado river estuary (NW Portugal). Groups of H. portulacoides (grown in a greenhouse) were exposed to those media during six days. Cu2+ (as Cu(NO3)2), 10(2) and 10(4) microg l(-1), was added to the media as well as 1.6 microg l(-1) of the sixteen EPA priority PAHs (0.1 microg l(-1) of each PAHs). Cu was assayed in solutions, sediments and different plant tissues before and after experiments. After exposure, photosynthetic efficiency and levels of chlorophylls were also measured, indicating that plant stress indicators were identical in all plants independently of the media to which the plants were exposed. PAHs influenced both the soluble Cu fraction and Cu uptake by plants. The amounts of metal accumulated in both roots and stems were significantly higher when the 10(4) microg l(-1) of Cu enriched elutriate was amended with PAHs. Thus, results suggest that PAHs may modify Cu solubility, the Cu sorption by plants and/or the passive penetration of Cu into the root cells. Therefore, the combined effects of different types of pollutants should be taken in consideration when studying the remediation potential of plants, namely in terms of phytoextraction.

Crenarchaeota and Euryarchaeota in temperate estuarine sediments

Aims: Application of molecular techniques to ecological studies has unveiled a wide diversity of micro‐organisms in natural communities, previously unknown to microbial ecologists. New lineages of Archaea were retrieved from several non‐extreme environments, showing that these micro‐organisms are present in a large variety of ecosystems. The aim was therefore to assess the presence and diversity of Archaea in the sediments of the river Douro estuary (Portugal), relating the results obtained to ecological data.

Bacterial community response to petroleum contamination and nutrient addition in sediments from a temperate salt marsh.

Microbial communities play an important role in the biodegradation of organic pollutants in sediments, including hydrocarbons. The aim of this study was to evaluate the response of temperate salt marsh microbial communities to petroleum contamination, in terms of community structure, abundance and capacity to degrade hydrocarbons. Sediments un-colonized and colonized (rhizosediments) by Juncus maritimus, Phragmites australis and Triglochin striata were collected in a temperate estuary (Lima, NW Portugal), spiked with petroleum under variable nutritional conditions, and incubated for 15 days. Results showed that plant speciation emerged as the major factor for shaping the rhizosphere community structure, overriding the petroleum influence. Moreover, when exposed to petroleum contamination, the distinct salt marsh microbial communities responded similarly with (i) increased abundance, (ii) changes in structure, and (iii) decreased diversity. Communities, particularly those associated to J. maritimus and P. australis roots displayed a potential to degrade petroleum hydrocarbons, with degradation percentages between 15% and 41%, depending on sediment type and nutritional conditions. In conclusion, distinct salt marsh microbial communities responded similarly to petroleum contamination, but presented different pace, nutritional requirements, and potential for its biodegradation, which should be taken into account when developing bioremediation strategies.

Impact of copper on the diversity, abundance and transcription of nitrite and nitrous oxide reductase genes in an urban European estuary

In this study, the effects of increasing copper (Cu) concentrations on the denitrification pathway and on the diversity of a denitrifier community and gene expression were evaluated in intertidal sandy sediments of the Douro River estuary (north-west Portugal). The results demonstrated that sediment denitrification rates were highly affected by Cu; almost complete inhibition (85%) of the process was observed in sediments amended with 60 μg Cu g(-1) wet wt sediment. Moreover, the addition of Cu stimulated the accumulation of both N(2)O and NO(2)(-) and inhibited the rate of NO(3)(-) uptake. Further, the amendment with even the lowest Cu concentration (4 μg Cu g(-1) wet wt sediment) yielded a drastic decrease in the abundance of nirK, nirS and nosZ (between 79% and 81%) assessed by means of real-time PCR. In agreement, reverse transcription-PCR-denaturing gradient gel electrophoresis analysis of nirK, nirS and nosZ transcripts showed a progressive decrease in the diversity of the transcription products of these genes with an increase of the Cu concentration.

Influence of surfactants on the Cu phytoremediation potential of a salt marsh plant

To assess the possible effect that surfactants commonly found in the aquatic environment may have on the remediation potential of the salt marsh plant Halimione portulacoides, a non-ionic (Triton X-100) and an anionic (sodium dodecyl sulfate, SDS) surfactants were used. Experiments were carried out in the laboratory, either in hydroponics (sediment elutriate) or in sediment soaked in elutriate, using sediment and water from an estuarine salt marsh (Cávado River, NW Portugal). Groups of H. portulacoides (grown in a greenhouse) were exposed for 6d to media with 0.16mM added Cu(II) in the absence and in the presence of each one of the two selected surfactants, at concentrations lower than the respective micellar critical concentration. Cu was determined in solutions, sediments and in different plant tissues before and after experiments. Plant photosynthetic efficiency did not indicate deletory effects of the exposure to the added pollutants. The non-ionic surfactant Triton X-100 and, to a lesser extent, the anionic surfactant SDS too, favored Cu accumulation in the plant roots but not Cu translocation, indicating that surfactants may favor Cu adsorption to the roots (phytostabilization). On the other hand, both surfactants favored Cu solubility from the sediment. Therefore, the presence of surfactants, which are frequently found in estuarine areas, as a result of urban and industrial effluent discharges, may condition metal distribution in those environments.

Influence of river discharge patterns on the hydrodynamics and potential contaminant dispersion in the Douro estuary (Portugal).

Freshwater input to estuaries is a fundamental feature of these ecosystems, which may be profoundly altered by river damming as human needs for water consumption, irrigation or energy production increase. The Douro estuary is limited upstream by a dam since 1985, which reduced its length by ca. 60%. Freshwater inputs to the estuary are now irregular and greatly dependent on hydroelectric power demand; values ranging from zero to over 1000m(3)s(-1), in a matter of hours, especially in summer are common. In the present study, a three-dimensional hydrodynamic model was applied to the Douro estuary. The model was calibrated and validated against water elevation, current velocity, salinity and temperature data. Thereafter, it was used to analyse the effects of different flow regimes and magnitudes on estuarine hydrodynamics and contaminant dispersion. Results obtained suggest that the highly variable flow regimes, currently observed in the Douro, tend to reduce water column stratification and to enhance seawater intrusion, when compared with flow discharges of similar average magnitude, but lower variability. Stable flows seem to be the most effective in dispersing contaminants eventually introduced into the estuary through its small river tributaries. Overall results suggest that flow management may have important effects on estuarine hydrodynamics through non-linear interactions between flow magnitude and variability.