Afaf Aboul Kheir

Quantitative HPLC analysis of mebeverine, mesalazine, sulphasalazine and dispersible aspirin stored in a Venalink monitored dosage system with co-prescribed medicines.

An HPLC method for the quantitative analysis of mebeverine HCl, 5-aminosalicylic acid (5-ASA), sulphasalazine and dispersible aspirin has been developed and then applied to these specific medicines when stored, with other medications, in Venalink blister packs (monitored dosage system) for periods of up to 35 days. Chromatographic separation was achieved on a reversed-phase C(12) column with an isocratic mixture of methanol, water and acetic acid as the mobile phase. The method was validated regarding: accuracy, precision, detection limits, quantification limits, specificity and robustness.

U.V. AND U.V. DERIVATIVE SPECTROPHOTOMETRIC DETERMINATION OF TWO-COMPONENT MIXTURES

Abstract In this paper, two-component mixtures (spironolactone with hydrochlorothiazide, captopril with hydrochlorothiazide, and spironolactone with furosemide) were assayed by U.V. (A), first derivative (dA/dlD) and second derivative (d2A/dlD2) spectrophotometric methods, applying “Zero-Crossing” technique of measurement. The methods were proved using laboratory prepared mixtures. The utility of the methods in assay of dosage forms is also presented.

Spectrophotometric determination of acetaminophen, oxyphenobutazone and salicylamide by nitration and subsequent complexation reactions

A simple and sensitive spectrophotometric method for the assay of three antipyretic drugs through their nitration and subsequent complexation with an nucleophilic reagent is proposed. The experimental conditions leading to optimum chromagen stability and intensity were studied. The results of the application of the method to the assay of the test compounds in unit doses were compared statistically with those obtained by official methods, and demonstrated good accuracy and precision.

Spectrophotometric determination of oxyphenbutazone by diazo coupling and subsequent chelation reactions

Two spectrophotometric procedures for the assay of oxyphenbutazone have been developed, based on coupling with a diazo reagent, reaction with copper(II) and extraction of the resulting chelate into chloroform. The absorbance of the chelate was measured and its copper(II) content determined by treatment with diethyldithiocarbamate reagent. The stoicheiometric ratios of the stepwise reactions were assessed by the continuous variations method and the assay parameters leading to quantitative results investigated. The results obtained are compared with those obtained by an official method.

The Use of Charge Transfer Complexation in the Spectrophotometric Determination of Some Corticosteroid Drugs Through Intermediate Oxime Formation

Abstract Two spectrophotometric procedures for the assay of three corticosteroid drugs through oximation and subsequent charge transfer complexation with two electron acceptor substances are proposed. The optimum experimental conditions, and molar ratio of reactants were studied. The applicability of the proposed procedures to assay the tested compounds in tablets from and comparison with an official method revealed the reliability, sensitivity and accuracy of the suggested procedures.

Spectrophotometric determination of some pharmaceutical carbonyl compounds through oximation and subsequent charge-transfer complexation reactions

Two spectrophotometric procedures are presented for the determination of six pharmaceutical carbonyl compounds through charge-transfer complexation of their oximes with either the σ-acceptor iodine or the π-acceptor choranil. The optimum assay conditions and their applicability to the determination of the test compounds in pharmaceutical products are described. The results obtained, which compared favourably with those given by a pharmacopoeial method, illustrate the accuracy, sensitivity and simplicity of the developed procedures.

Atomic Absorption Spectrometry of Flufenamic Acid

Abstract A simple and accurate method was described for the quantitative determination of flufenamic acid utilizing precipitation reactions with silver (I), copper (II) and iron (III). Flufenamic acid was precipitated from its neutral alcoholic solution by silver nitrate, copper acetate or ferric chloride standard solutions followed by direct determination of the above cited ions in the precipitate or its indirect determination in the filtrate applying atomic absorption spectroscopy. The optimum conditions for precipitation have been carefully studied. The molar ratio of the reactants were ascertained. Statistical analysis of the results obtained compared to the results of the official method revealed equal precision and accuracy. The suggested procedures were applied for determining flufenamic acid in pharmaceutical preparations as well and proved validity.

A Sensitive Spectrophotometric Method for the Determination of Progesterone, Testosterone Propionate and Nandrolone Phenylpropionate in Ampoules

Abstract A sensitive spectrophotometric method for the determination of three kdetosteriod drugs is suggested. The method is based on oximation, separation and acid cleavage of their oximes followed by spectro-photometric measurement of the liberated hydroxylamine through oxidation and diazo coupling reactions. The method gave mean percent recoveries of 98.92 ± 0.58, 99.26 ± 0.96, and 99 10 ± 0.47 for progesterone, testosterone propionate and nandrolone phenyl propionate respectively in ampoules. Results were in good agreement with B.P. 1980 method.