Afifah Bambang Sutjiatmo

Angiotensin Converting Enzyme Inhibitor Activity of Ethanol Extract of Sonchus Arvensis (Linn.) Leaves

Objective: This research was to know the activity of Ethanol extract of Sonchus arvensis (Linn.) leaves in inhibiting the angiotensin converting enzyme (ACE) activity. ACE is an enzyme that functions to convert the Angiotensin I into Angiotensin II. The Angiotensin II has a role in the process of hypertension, it has a strong vasoconstriction effect inducting the hypertension. Methods: The ethanol extract of Sonchus arvensis (Linn.) was made through the maceration method with ethanol 96% as solvent. The inhibition extract activity of ACE activity was evaluated using N-hippuryl-L-histidyl-L-leucine (HHL) as a substrate and will be cleaved by ACE. The inhibitory activity of ethanol extract of Sonchus arvensis (Linn.) leaves towards ACE was calculated based on the number of hypural acid was created and calculated based on the absorbance value at 228 nm wavelength measured using spectrophotometer UV. The results: The ethanol extract of Sonchus arvensis (Linn.) has inhibition activity ACE at IC50 46.71 μg/ml and IC50 from captopril as a reference drug in 1,26 μg/ml. Therefore, it can be said that the inhibitory activity of Ethanol extract was lower that captopril. Conclusion: Sonchus arvensis (Linn.) is potentially used as the ACE inhibitor and is potential to be developed as antihypertension.

UJI EFEK ANTITUKAK LAMBUNG EKSTRAK AIR HERBA BAYAM MERAH(Amaranthus tricolor L.)TERHADAP TIKUS WISTAR BETINA

Herba bayam merah ( Amaranthus tricolor Linn) dilaporkan dapat mengatasi gangguan lambung yang disebabkan asam berlebih. Penelitian ini bertujuan untuk menguji aktivitas antitukak lambung ekstrak air herba bayam merah. Ekstrak air herba bayam merah dibuat dengan cara perendaman pada air dingin selama 2 jam, selanjutnya disaring dan filtrat dikeringkan menggunakan freeze dry . Dosis ekstrak air herba bayam merah yang digunakan adalah 47,5; 95, dan 190 mg/kg bb, dan digunakan simetidin72 mg/kg bb sebagai pembanding. Induksi tukak lambung dilakukan dengan pemberian asetosal500 mg/kg bb dan pengikatan pilorus. Parameter yang diukur adalah keasaman lambung, jumlah tukak, diameter tukak, indeks tukak. Hasil penelitian menunjukkan ekstrak air dosis 47,5; 95, dan 190 mg/kg bb mampu menurunkan keasaman lambung, menurunkan jumlah tukak lambung dan memperkecil diameter tukak lambung jika dibandingkan kelompok kontrol. Dosis ekstrak air herba bayam merah yang paling efektif sebagai anti tukak lambung adalah 190 mg/kg bb dan setara dengan pembanding simetidin 72 mg/kg bb (p>0,05).

Hypoglycaemic activity of aqueous extract of leafs of Strobilanthes crispa L. in male Wistar rats using glucose tolerance test method

ABSTRACT Extract of has been used traditionally to treat diabetes mellitus and gallstones. The objective of this study is to test hypoglycemic effect of aqueous extract of leafs of S. crispa in rats using glucose tolerance test method. Aqueous extract of leafs of S. crispa L. at doses 150 mg/kg bw and 300 mg/kg bw were orally administered in male Wistar rat. Blood glucose rate was analysed every 60 minutes over 3 hours. As conclusion, aqueous extract of leafs of S. crispa at dose 150 mg/kg bw gave similar effect with chlorpropamid at dose 9 mg/kg bw. ABSTRAK Daun Strobilanthes crispa L. digunakan dalam pengobatan tradisional untuk mengatasi diabetes dan batu ginjal. Penelitian ini bertujuan untuk mengetahui efek hipoglikemik ekstrak air daun Strobilanthes crispa L. pada tikus betina galur Wistar dengan metode toleransi glukosa. Ekstrak dosis 150 dan 300 mg/kg bb masing-masing diberikan p.o pada tikus betina. Analisa peningkatan glukosa darah dilakukan setiap 60 menit selama 3 jam. Kesimpulan penelitian ini, ekstrak air daun Strobilanthes crispa L. dosis 150 mg/kg bb memiliki efek yang setara dengan klorpropamid dosis 9 mg/kg bb.