Agnieszka Kaczor
Jagiellonian University
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Publication
Featured researches published by Agnieszka Kaczor.
Journal of Neuroscience Methods | 2002
Jonas Bergquist; Agnieszka Sciubisz; Agnieszka Kaczor; Jerzy Silberring
Catecholamines act via dopaminergic-, and adrenergic receptors, and are involved in a variety of regulatory systems. They take part in regulation of the response to stress, psychomotor activity, emotional processes, learning, sleep and memory. Due to many catecholaminergic pathways, and a wide range of functions they are involved in, both in the central nervous system and in periphery, a development of the reliable techniques for their extraction and quantitation is essential. This paper presents an overview of the currently applied methodologies for catecholamines detection and identification in various biological samples.
Analyst | 2011
Agnieszka Kaczor; Katarzyna Turnau; Malgorzata Baranska
Raman imaging is shown to be a highly selective and sensitive method of studying in situ and in vivo astaxanthin distribution, concentration and molecular structure in the cyst form of the unicellular microalgae Haematococcus pluvialis.
Analytical Chemistry | 2011
Agnieszka Kaczor; Malgorzata Baranska
The changes of structure of astaxanthin (AXT), a superpotent antioxidant, upon thermal stress were investigated in unicellular microalgae Haematococcus pluvialis by measuring Raman spectra in situ and analyzing obtained results with DFT calculations. Although no visual changes are observed in the Haematococcus cells upon heating, discernible changes in Raman spectra occur from -100 °C systematically up to 150 °C. The exponential increase of the Raman shift of the ν C═C band at ca. 1520 cm(-1) along with the change of the intensity ratio of bands at 1190 and 1160 cm(-1) is observed, that correlates with the changes predicted by calculations for astaxanthin conformers ordered by decreasing energy. It is assumed that AXT molecules, initially in the form of H-aggregates with the trans conformations of the end-rings, interconvert toward more stable gauche forms upon thermal stress of the algae. The applied approach enables one to follow structural changes of the carotenoid upon temperature stress both in a single algal cell and in a multicellular sample in situ. Obtained information might be of use to improve the industrial process of extraction of AXT in its most bioavailable form.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2017
Ewelina Wiercigroch; Ewelina Szafraniec; Krzysztof Czamara; Marta Z. Pacia; Katarzyna Majzner; Kamila Kochan; Agnieszka Kaczor; Malgorzata Baranska; Kamilla Malek
Carbohydrates are widespread and naturally occurring compounds, and essential constituents for living organisms. They are quite often reported when biological systems are studied and their role is discussed. However surprisingly, up till now there is no database collecting vibrational spectra of carbohydrates and their assignment, as has been done already for other biomolecules. So, this paper serves as a comprehensive review, where for selected 14 carbohydrates in the solid state both FT-Raman and ATR FT-IR spectra were collected and assigned. Carbohydrates can be divided into four chemical groups and in the same way is organized this review. First, the smallest molecules are discussed, i.e. monosaccharides (d-(-)-ribose, 2-deoxy-d-ribose, l-(-)-arabinose, d-(+)-xylose, d-(+)-glucose, d-(+)-galactose and d-(-)-fructose) and disaccharides (d-(+)-sucrose, d-(+)-maltose and d-(+)-lactose), and then more complex ones, i.e. trisaccharides (d-(+)-raffinose) and polysaccharides (amylopectin, amylose, glycogen). Both Raman and IR spectra were collected in the whole spectral range and discussed looking at the specific regions, i.e. region V (3600-3050cm-1), IV (3050-2800cm-1) and II (1200-800cm-1) assigned to the stretching vibrations of the OH, CH/CH2 and C-O/C-C groups, respectively, and region III (1500-1200cm-1) and I (800-100cm-1) dominated by deformational modes of the CH/CH2 and CCO groups, respectively. In spite of the fact that vibrational spectra of saccharides are significantly less specific than spectra of other biomolecules (e.g. lipids or proteins), marker bands of the studied molecules can be identified and correlated with their structure.
PLOS ONE | 2014
Marta Pilarczyk; Lukasz Mateuszuk; Anna Rygula; Mariusz Kepczynski; Stefan Chlopicki; Malgorzata Baranska; Agnieszka Kaczor
Lipid rafts (LRs) are dynamic, sterol- and sphingolipid-enriched nanodomains involved in the regulation of cellular functions and signal transduction, that upon stimuli, via (e.g. association of raft proteins and lipids), may cluster into domains of submicron or micron scale. Up to date, however, lipid raft clusters were observed only under artificially promoted conditions and their formation in vivo has not been confirmed. Using non-destructive approach involving Raman and Atomic Force Microscopy imaging we demonstrated the presence of clustered lipid rafts in endothelium of the aorta of the db/db mice that represent a reliable murine model of type 2 diabetes. The raft clusters in the aorta of diabetic mice were shown to occupy a considerably larger (about 10-fold) area of endothelial cells surface as compared to the control. Observation of pathology-promoted LRs confirms that the cellular increase of lipid content results in clustering of LRs. Clustering of LRs leads to the formation of assemblies with diameters up to 3 micrometers and increased lipid character. This massive clustering of lipid rafts in diabetes may trigger a signaling cascade leading to vascular inflammation.
Scientific Reports | 2017
Krzysztof Czamara; Katarzyna Majzner; Anna Selmi; Malgorzata Baranska; Yukihiro Ozaki; Agnieszka Kaczor
Endothelial HMEC-1 cells incubated with pro-inflammatory cytokine TNF-α for 6 and 24 hours were studied as a model of inflammation using Raman imaging. Striking changes in distribution, composition and concentration of cellular lipids were observed after exposure to TNF-α compared to the control. In particular, 3D Raman imaging revealed a significant increase in the amount of lipid entities formed under inflammation. Lipid bodies were randomly distributed in the cytoplasm and two types of droplets were assembled: more saturated one, in spectral characteristics resembling phosphatidylcholine and saturated cholesteryl esters, observed also in the control, and highly unsaturated one, containing also cholesterols, being a hallmark of inflamed cells. The statistical analysis showed that the number of lipid bodies was significantly dependent on the exposure time to TNF-α. Overall, observed formation of unsaturated lipid droplets can be directly correlated with the increase in production of prostacyclins - endogenous inflammation mediators.
Pharmacological Reports | 2015
Malgorzata Baranska; Agnieszka Kaczor; Kamilla Malek; Aleksandra Jaworska; Katarzyna Majzner; Emilia Staniszewska-Slezak; Marta Z. Pacia; Grzegorz Zajac; Jakub Dybas; Ewelina Wiercigroch
Raman microscopy, a label-free method with high spatial resolution, shows growing potential in various fields of medical diagnostics. Several proof-of-concept studies related to the application of Raman microscopy to detect endothelial dysfunction are summarized in this work. Both ex vivo measurements of the tissues in the murine models of endothelial pathologies, as well as in vitro investigations of the cell cultures in the context of cellular transport, drug action and inflammation processes are discussed. The future directions in application of Raman spectroscopy-based methods in such studies are also described.
Journal of Physical Chemistry B | 2016
Grzegorz Zajac; Agnieszka Kaczor; Ana Pallares Zazo; Jacek Mlynarski; Monika Dudek; Malgorzata Baranska
Raman optical activity (ROA) spectroscopy is hampered by low sensitivity, with limited possibilities for enhancing the signal. In the present study, we report a new mechanism whereby chirality is enhanced using the resonance resulting from supramolecular aggregation. We have named this mechanism aggregation-induced resonance Raman optical activity (AIRROA). As an example, we study J-aggregates of astaxanthin (AXT), which show strong absorption of circularly polarized light in the range of ROA excitation. The implications of aggregation-induced signal enhancement for chiroptical spectroscopy are discussed.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2011
Elżbieta Szostak; Anna Migdał-Mikuli; Agnieszka Kaczor; Wojciech Nitek
Single crystal X-ray diffraction studies of [Mn(OS(CH3)2)6](ClO4)2 have shown that the low temperature phase transition, detected by differential scanning calorimetry (DSC) at about 223 K, is associated with the crystal symmetrys reduction from an orthorhombic crystallographic system (Fdd2, No. 43) to a monoclinic one (Cc, No. 9). The analysis of the full width at half maximum of the bands connected with: δd(OClO)F2 and ρ(CH3) vibrational modes in the FT-IR and FT-RS spectra, respectively, registered in the function of temperature, proved that the reorientational motions of ClO4- anions and CH3 groups from (CH3)2SO ligands, began to slow down at temperatures below the phase transition at about 223K. Mean values of activation energy for ClO4- reorientation in the high temperature phase I and low temperature phase II are: Ea(I)≈14 kJ mol(-1) and Ea(II)≈10 kJ mol(-1), respectively. Analogous values for CH3 reorientation are: Ea(I)≈23 kJ mol(-1) and Ea(II)≈1 kJ mol(-1), respectively.
Archive | 2016
Agnieszka Kaczor; Malgorzata Baranska
Carotenoids: Nutrition, Analysis and Technology is an up-to-date overview of the key areas of carotenoids in nutrition, therapy and technology. In the first section, the authors present a functional food perspective, outlining the therapeutic applications of the bioactive pigments. The second part is dedicated to the spectroscopic analysis of carotenoids, providing in-depth scientific methods and real research findings. In the final section, various technological applications of carotenoids are considered, including biotechnology and future prospects.