Agui Hideo
Sumitomo Chemical
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Publication
Featured researches published by Agui Hideo.
Gene | 1986
Kishimoto Fumitaka; Gomi Hideyuki; Kanaoka Masaharu; Nakatani Tomoyuki; Ito Akira; Katoh Tomoko; Agui Hideo; Sumida Seizo; Ogino Shigeo
Abstract Human epidermal growth factor (urogastrone; UG) is a 53-amino acid polypeptide hormone. A 192-bp DNA fragment containing the coding sequence for methionyl UG (Met-UG) and the ribosome-binding site (RBS) was chemically synthesized and placed downstream from the promoter for the Escherichia coli outer-membrane lipoprotein gene (Ipp) on a plasmid. E. coli cells harboring the plasmid directed the synthesis of Met-UG at 10 2 -10 2 molecules per cell. Next, the coding sequence for Met-UG was inserted in a runaway-replication plasmid and expressed under the control of the lpp promoter and the RBS derived from bacteriophage Mu cII gene. Upon heat induction, the cells harboring the recombinant plasmid synthesized 10 5 molecules of Met-UG per cell.
Archive | 1984
Agui Hideo; Saji Ikutaro; Nakashita Mitsuo
Archive | 1982
Agui Hideo; Saji Ikutaro; Nakashita Mitsuo
Archive | 1993
Sato Hideshi; Takahashi Sene; Negoro Takaharu; Sudo Yoshiaki; Agui Hideo
Archive | 1991
Jigami Yoshifumi; Uemura Hiroshi; Muraki Michirou; Tanaka Hideaki; Nakazato Satoshi; Kishimoto Fumitaka; Agui Hideo; Ogino Shigeo
Archive | 1991
Kanaoka Masaharu; Negoro Takaharu; Kawanaka Chigusa; Agui Hideo
Archive | 1987
Agui Hideo; Tamoto Katsumi; Aono Shunji; Okuda Takao
Archive | 1985
Saji Ikutaro; Tamoto Katsumi; Aono Shunji; Achise Katsuski; Okuda Takao; Agui Hideo
Archive | 1985
Agui Hideo; Tamoto Katsumi; Aono Shunji; Okuda Takao
Archive | 1984
Saji Ikutaro; Aono Shunji; Okuda Takao; Agui Hideo
