Ahmed Ghoneima

Three-dimensional computed tomography analysis of airway volume changes after rapid maxillary expansion

INTRODUCTION In this retrospective study with 3-dimensional computed tomography, we evaluated airway volume, soft-palate area, and soft-tissue thickness changes before and after rapid maxillary expansion in adolescents. Another purpose was to determine whether rapid maxillary expansion caused changes in the palatal and mandibular planes and facial height. METHODS The sample comprised 20 patients who were treated with rapid maxillary expansion. Spiral tomographs were taken before and 3 months after treatment. Reliability studies were performed, and then volumetric, soft-palate area, soft-tissue thickness, and cephalometric parameters were compared on the tomographs. Intraclass correlations were performed on the reliability measurements. Before and after rapid maxillary expansion measurements were compared by using Wilcoxon signed rank tests. Spearman correlation coefficients were used to evaluate the associations among the airway volume, soft-palate area, soft-tissue thickness, and cephalometric measurements. Significance was accepted at P ≤0.05 for all tests. RESULTS Intraclass correlation coefficients were ≥0.90 for all reliability measures. Significant increases from before to after rapid maxillary expansion were found in nasal cavity and nasopharynx volumes, and for the measurements of MP-SN, S-PNS, N-ANS, ANS-Me, and N-Me. Significant positive correlations existed between changes in PP-SN and N-ANS, and ANS-Me and N-Me. CONCLUSIONS Rapid maxillary expansion causes significant increases in nasal cavity volume, nasopharynx volume, anterior and posterior facial heights, and palatal and mandibular planes.

Accuracy and reliability of cone-beam computed tomography for airway volume analysis

The purpose of the present study was to evaluate the accuracy and reliability of airway volume digital measurements of cone-beam computed tomography (CBCT) compared with the manual measurements of an airway model. An acrylic airway model was constructed and attached to a human dry skull in the natural position of the airway passage. The total and internal airway volumes, as well as the most constricted airway area, were measured manually on the model and on the CBCTs taken after the model was attached to the skull. The CBCT images were analysed using the Dolphin3D (Dolphin Imaging & Management solutions, Chatsworth, California, USA) software. Reliability and accuracy were assessed by using intraclass correlation and Students t-test. A P ≤ 0.05 was considered statistically significant. No significant statistical difference was found between the total, the internal airway volume, and the most constricted airway area measured on CBCTs compared with the manual measurements. The intra-examiner reliability was high for all measurements recorded from both methods (r ≥ 0.90). These results suggested that the three-dimensional CBCT digital measurements of the airway volume and the most constricted area of the airway are reliable and accurate. The use of CBCT imaging for the assessment of the airway can provide clinically useful information in orthodontics.

Effects of rapid maxillary expansion on the cranial and circummaxillary sutures.

INTRODUCTION The aim of this study was to determine whether the orthopedic forces of rapid maxillary expansion cause significant quantitative changes in the cranial and the circummaxillary sutures. METHODS Twenty patients (mean age, 12.3 ± 1.9 years) who required rapid maxillary expansion as a part of their comprehensive orthodontic treatment had preexpansion and postexpansion computed tomography scans. Ten cranial and circummaxillary sutures were located and measured on one of the axial, coronal, or sagittal sections of each patients preexpansion and postexpansion computed tomography scans. Quantitative variables between the 2 measurements were compared by using the Wilcoxon signed rank test. A P value less than 0.05 was considered statistically significant. RESULTS Rapid maxillary expansion produced significant width increases in the intermaxillary, internasal, maxillonasal, frontomaxillary, and frontonasal sutures, whereas the frontozygomatic, zygomaticomaxillary, zygomaticotemporal, and pterygomaxillary sutures showed nonsignificant changes. The greatest increase in width was recorded for the intermaxillary suture (1.7 ± 0.9 mm), followed by the internasal suture (0.6 ± 0.3 mm), and the maxillonasal suture (0.4 ± 0.2 mm). The midpalatal suture showed the greatest increase in width at the central incisor level (1.6 ± 0.8 mm) followed by the increases in width at the canine level (1.5 ± 0.8 mm) and the first molar level (1.2 ± 0.6 mm). CONCLUSIONS Forces elicited by rapid maxillary expansion affect primarily the anterior sutures (intermaxillary and maxillary frontal nasal interfaces) compared with the posterior (zygomatic interface) craniofacial structures.

Bisphosphonates treatment and orthodontic considerations.

Currently, the use of oral and systemic forms of bisphosphonates is increasing dramatically in a large group of patients either in the form of anti-osteoporosis medications or as a part of a chemotherapeutic regimen for several malignant diseases. As adult orthodontic treatment has become more widely accepted in most orthodontic practices, orthodontists must be aware of the risks, benefits, and effects of bisphosphonates use on the patients general health status, as well as on their orthodontic treatment outcomes. This review aims to discuss the use of bisphosphonates, the complications associated with their use, and their impact on orthodontic treatment.

The reliability and reproducibility of cephalometric measurements: a comparison of conventional and digital methods

OBJECTIVE The aim of this study was to assess the reliability and reproducibility of angular and linear measurements of conventional and digital cephalometric methods. METHODS A total of 13 landmarks and 16 skeletal and dental parameters were defined and measured on pre-treatment cephalometric radiographs of 30 patients. The conventional and digital tracings and measurements were performed twice by the same examiner with a 6 week interval between measurements. The reliability within the method was determined using Pearsons correlation coefficient (r²). The reproducibility between methods was calculated by paired t-test. The level of statistical significance was set at p < 0.05. RESULTS All measurements for each method were above 0.90 r² (strong correlation) except maxillary length, which had a correlation of 0.82 for conventional tracing. Significant differences between the two methods were observed in most angular and linear measurements except for ANB angle (p = 0.5), angle of convexity (p = 0.09), anterior cranial base (p = 0.3) and the lower anterior facial height (p = 0.6). CONCLUSION In general, both methods of conventional and digital cephalometric analysis are highly reliable. Although the reproducibility of the two methods showed some statistically significant differences, most differences were not clinically significant.

In vivo effects of zoledronic acid on oral mucosal epithelial cells.

OBJECTIVE Osteonecrosis of the jaw is a serious complication of bisphosphonate treatment for which the pathophysiology is unknown. The purpose of this study was to investigate whether in vivo zoledronic acid (ZA) induces alterations in cell proliferation, apoptosis, and matrix metalloproteinases (MMPs) expression in oral mucosal epithelial cells. METHODS One-year-old dogs were either untreated (control group) or given high doses of intravenous ZA (ZA group) for 3 months. The doses of ZA were equivalent to those given to cancer patients, yet were administered two times more frequently (every 2 weeks). Mucosal tissues were assessed immunohistochemically for cell proliferation (proliferating cell nuclear antigen, PCNA), matrix metalloproteinase (MMP) expression, and apoptosis (caspase 3 and TUNEL). RESULTS There were no significant differences between the groups with respect to PCNA, MMP-2, MMP-14, and TUNEL positive cells. However, the expression of MMP-9 was significantly higher in the control group than in the ZA group (P < 0.05), whereas the expression of caspase 3 was significantly lower in the control group than in the ZA group (P < 0.05). CONCLUSION   These results suggest that high doses of ZA resulted in higher levels of apoptosis and lower levels of MMP-9 in the oral epithelial cells supporting the idea of bisphosphonate treatment affects the oral mucosa.

Orthodontic soft-tissue parameters: A comparison of cone-beam computed tomography and the 3dMD imaging system

INTRODUCTION Orthodontists rely heavily on soft-tissue analysis to determine facial esthetics and treatment stability. The aim of this retrospective study was to determine the equivalence of soft-tissue measurements between the 3dMD imaging system (3dMD, Atlanta, Ga) and the segmented skin surface images derived from cone-beam computed tomography. METHODS Seventy preexisting 3dMD facial photographs and cone-beam computed tomography scans taken within minutes of each other for the same subjects were registered in 3 dimensions and superimposed using Vultus (3dMD) software. After reliability studies, 28 soft-tissue measurements were recorded with both imaging modalities and compared to analyze their equivalence. Intraclass correlation coefficients and Bland-Altman plots were used to assess interexaminer and intraexaminer repeatability and agreement. Summary statistics were calculated for all measurements. To demonstrate equivalence of the 2 methods, the difference needed a 95% confidence interval contained entirely within the equivalence limits defined by the repeatability results. RESULTS Statistically significant differences were reported for the vermilion height, mouth width, total facial width, mouth symmetry, soft-tissue lip thickness, and eye symmetry. CONCLUSIONS There are areas of nonequivalence between the 2 imaging methods; however, the differences are clinically acceptable from the orthodontic point of view.

Effects of tobacco on cytokine expression from human endothelial cells

OBJECTIVE To investigate the effects of nicotine and cigarette smoke condensate (CSC) exposure on cytokine expression from human endothelial cells in order to identify one possible mechanism that smoking plays in the pathogenesis of both periodontal disease (PDD) and cardiovascular disease (CVD). METHODS Human endothelial cells (HUVECs) were exposed to different concentrations of nicotine and CSC to examine the effects that they have on cell proliferation and cytotoxicity. Non-toxic levels were then used to examine cytokine expression using cytokine protein arrays. RESULTS Exposure to nicotine caused significant down-regulation in the expression of IL-10 (P = 0.046), growth-regulated oncogene (GRO)α (P = 0.036), MCP-1 (P = 0.046), and GMCSF (P = 0.004) compared with the control untreated HUVECs. Exposure to CSC caused significant down-regulation in the expression of GRO (P = 0.04), GROα (P = 0.01), IL-6 (P = 0.03), and MCP-1 (P = 0.04) compared with the control untreated HUVECs. CONCLUSIONS Exposure of HUVECs to nicotine or CSC affects the levels of cytokine expression including reduction in anti-inflammatory and chemoattractant cytokines. This may subsequently affect the host defensive mechanisms of the tissues. The action of toxic chemicals in tobacco smoke on endothelial cells is a potential pathogenic mechanism that may in part explain the association between tobacco, PDD, and CVD.

Nasal airway and septal variation in unilateral and bilateral cleft lip and palate

Cleft lip and palate (CLP) affects the dentoalveolar and nasolabial facial regions. Internal and external nasal dysmorphology may persist in individuals born with CLP despite surgical interventions. 7–18 year old individuals born with unilateral and bilateral CLP (n = 50) were retrospectively assessed using cone beam computed tomography. Anterior, middle, and posterior nasal airway volumes were measured on each facial side. Septal deviation was measured at the anterior and posterior nasal spine, and the midpoint between these two locations. Data were evaluated using principal components analysis (PCA), multivariate analysis of variance (MANOVA), and post‐hoc ANOVA tests. PCA results show partial separation in high dimensional space along PC1 (48.5% variance) based on age groups and partial separation along PC2 (29.8% variance) based on CLP type and septal deviation patterns. MANOVA results indicate that age (P = 0.007) and CLP type (P ≤ 0.001) significantly affect nasal airway volume and septal deviation. ANOVA results indicate that anterior nasal volume is significantly affected by age (P ≤ 0.001), whereas septal deviation patterns are significantly affected by CLP type (P ≤ 0.001). Age and CLP type affect nasal airway volume and septal deviation patterns. Nasal airway volumes tend to be reduced on the clefted sides of the face relative to non‐clefted sides of the face. Nasal airway volumes tend to strongly increase with age, whereas septal deviation values tend to increase only slightly with age. These results suggest that functional nasal breathing may be impaired in individuals born with the unilateral and bilateral CLP deformity. Clin. Anat. 27:999–1008, 2014.

Computational fluid dynamics analysis of the upper airway after rapid maxillary expansion: a case report.

BackgroundAssessment of the upper airway volume, morphology, and mechanics is of great importance for the orthodontic patient. We hypothesize that upper airway dimensions have significant effects on the dynamics of the airway flow and that both the dimensions and mechanics of the upper airway are greatly affected by orthodontic and orthopedic procedures such as rapid maxillary expansion (RME). The aim of the current study was to assess the effect of RME on the airway flow rate and pattern by comparing the fluid dynamics results of pre- and post-treatment finite element models.MethodsCustomized pre- and post-treatment computational fluid dynamics models of the patient’s upper airway were built for comparison based on three-dimensional computed tomogram. The inhalation process was simulated using a constant volume flow rate for both models, and the wall was set to be rigid and stationary. Laminar and turbulent analyses were applied.ResultsComparisons between before and after RME airway volume measurements showed that increases were only detected in nasal cavity volume, nasopharynx volume, and the most constricted area of the airway. Pressure, velocity, and turbulent kinetic energy decreased after dental expansion for laminar and turbulent flow. Turbulent flow shows relatively larger velocity and pressure than laminar flow.ConclusionsRME showed positive effects that may help understand the key reasons behind relieving the symptom of breathing disorders in this patient. Turbulence occurs at both nasal and oropharynx areas, and it showed relatively larger pressure and velocity compared to laminar flow.