Ahmet G. Önol

Effects of probiotics: alone and in a mixture of Biosacc plus Zinc Bacitracin on the caecal microflora of Japanese quail

The aim of this study was to investigate the effects of two commercially available probiotics, alone and in combination with an antibiotic, on the caecal flora of Japanese quail (Coturnix coturnix japonica) reared under unstressed conditions. Thirty-four 90-day-old Japanese quail were selected for this study. The birds were divided into four groups, two groups of nine birds and two groups of eight birds. The animals in these groups were given feed containing 0.5 kg per tonne of probiotics alone (Protexin or Biosacc), a mixture of probiotic plus antibiotic (Biosacc plus Zinc Bacitracin at 0.5 and 0.0525 ppm, respectively), and a group where no supplement was added to be used as controls. The total count of aerobic bacteria, lactobacilli, enterobacteriaceae, coliforms, enteroccoci, salmonellae, sulphite-reducing anaerobic bacteria (clostridia), and pH values in the caecal content of the birds were examined. No significant differences were detected among the four groups for pH values and bacterial number (p>0.05), except for sulphite-reducing anaerobic bacteria (p<0.001). These results suggest that the use of probiotics alone and/or a mixture of a probiotic plus antibiotic as a feed supplement does not have a major suppressing effect on the majority of bacterial groups in the caecal flora of mature, healthy Japanese quail reared in unstressed conditions.

Supplemental boric acid does not prevent the development of footpad dermatitis in broilers subjected to high stocking density

ABSTRACT The present study was conducted to evaluate the protective effect of dietary boric acid supplementation on the development of incidence and severity of footpad dermatitis (FPD) in broiler chickens subjected to normal or high stocking densities (NSD or HSD). A total of 576 1‐day‐old ROSS 308 broiler chickens were randomly allocated to 4 treatments (8 replicate pens per treatment) in a 2 × 2 factorial arrangement of dietary boric acid (0 and 60 mg/kg) and stocking density (NSD 14 birds/m2 and HSD 22 birds/m2). Basal diets were formulated for starter, grower, and finisher phases. Growth performance, litter quality (litter pH, moisture, temperature, and NH3 volatilization), serum and litter boron levels, and incidence and severity of FPD were recorded. The HSD affected the body weight gain and feed intake of broiler chickens during all phases and 0 to 42 (P < 0.05), whereas feed conversion ratio (FCR) was poor at 0 to 21 days only. Dietary boric acid had no effect on the growth performance of broiler chickens. Litter pH, moisture, and NH3 volatilization were higher in broiler chickens subjected to HSD (P < 0.05). Thus, the incidence and severity of FPD increased in response to HSD (P < 0.05). Dietary boric acid reduced the litter pH and NH3 volatilization on day 42 of experiment (P < 0.05). However, dietary boric acid supplementation had no effect on the incidence and severity of FPD. Boric acid supplementation in broiler diets increased the serum and litter boron levels at day 42 in broiler chickens subjected to NSD or HSD (P < 0.05). In conclusion, HSD resulted in poor growth performance, litter quality, and greater incidence and severity of FPD in broiler chickens. Dietary boric acid was ineffective against FPD in broiler chickens although it improved the litter quality by lowering the litter pH and NH3 volatilization.