Ahmet Serteser

Determination of antioxidant effects of some plant species wild growing in Turkey

In this study, the antioxidant activity of 50% aqueous methanol extracts of Crataegus tanacetifolia (Lam.) Pers, Crataegus bornmuelleri Zaberi, Crataegus orientalis Pall. ex M.Bieb. var. orientalis, Crataegus szovitsii Pojark, Crataegus curvisepala Lindm., Crataegus monogyna Jacq. subsp. monogyna, Crataegus monogyna Jacq.subsp. azarella (Gris.) Franco, Crataegus micophylla C.Koch, Rosa foetida Herrm., Rosa hemisphaerica J.Herrm., Rosa pulverulenta M.Bieb., Rosa canina L., Rubus discolor Weihe & Nees, Rubus canescens DC. var. canescens, Rubus sanctus Screber, Rubus caesius L., Sorbus umbellata (Desf.) Fritsch var. umbellata, Prunus avium L. (Moench.) and Prunus cerasus L. Mespilus germenica was evaluated by various antioxidant assays, including free radical scavenging, hydrogen peroxide scavenging and metal-chelating activities. The extracts of R. hemisphaerica J.Herrm., P. cerasus L. and R. canina L. showed more stronger free radical scavenging and hydrogen peroxide scavenging activities, and the extracts of R. foetida Herrm. (62.54%) and P. cerasus L. showed stronger metal-chelating activity. The results obtained in the present study indicated that the R. hemisphaerica J.Herrm., P. cerasus L. and R. canina are potential sources of natural antioxidant. These antioxidant properties depend on the concentration of the sample.

Relationships between epipelic diatoms, aquatic macrophytes, and water quality in Akarçay Stream, Afyonkarahisar, Turkey

The Akarçay is the most important stream in the Afyonkarahisar region in central Turkey. In this study, the relationships between epipelic diatoms, macrophytes, and the water quality of Akarçay Stream are presented. According to Canonical Correspondence Analysis (CCA), BOD5, COD, TDS, NH4-N, NO2-N, and PO4-P had the most significant effects on the diatom and macrophyte community structures of the stream. According to CCA and Correspondence Analysis (CA), dominant diatom species and macrophytes constituted two groups, eutrophic and polluted. While eutraphentic taxa were abundant among upstream diatoms, hypereutraphentic taxa were found downstream in the Akarçay. The diatom assemblages in the sites upstream were generally characterized by an abundance of β-mesosaprobous and α-mesosaprobous species, while the diatom assemblages downstream had a very high abundance of polisaprobous species. Eutrophic and hypertrophic macrophyte taxa (such as Lemna trisulca, Potamogeton nodosus, and Ranunculus sp.) covered a high percentage of the stream bottom. Both diatom indices and macrophyte indices with the exception of the Biological Macrophyte Index of Rivers (IBMR) were correlated with BOD5, COD, TDS, NH4-N, NO2-N, PO4-P, and EC. Physicochemical variables, diatom and macrophyte taxa and the results of diatom and macrophyte indices indicated that the Akarçay stream basin is eutrophic and organically polluted.

Antioxidant Activity of Ribes multiflorum Kit. ex Roem. & Schult (blackcurrant) Extract

Abstract In this study, the antioxidant contents of Ribes multiflorum Kit. Ex Roem. & Schult (black currant) which was grown in Emir and Kumalar mountains in Afyonkarahisar were investigated. Ribes multiflorum Kit. Ex Roem. & Schult is found on 1700 m at Emir Mountain in Dumluca, on 1400 and 1600 m at Kumalar Mountains in Basören. Antioxidant activities of samples were determined by free radical scavenging effect, chelating activity with Fe +2 and H2O2 inhibition methods. The lower EC50 value, the higher antioxidant power. In the presence of metal ions, super reactive hydroxyl radical may be formed by Fenton reaction. The findings obtained in the present work indicated that the Ribes multiflorum are potential source of natural antioxidant. Their antioxidant properties probably depend on concentration of sample.

Genotoxic evaluation of Halfenprox using the human peripheral lymphocyte micronucleus assay and the Ames test

Abstract The genotoxicity and mutagenicity of Halfenprox, a synthetic pyrethroid insecticide and acaricide, was assessed using two standard genotoxicity assays of the Salmonella typhimurium mutagenicity assay (Ames test) and in vitro micronucleus (MN) assay in human peripheral lymphocytes. In the Ames test, Salmonella strains TA98 and TA100 were treated with or without S9 fraction. The doses of Halfenprox were 6.25, 12.5, 25, 50, and 100 μg/plate and test materials were dissolved in DMSO. The concentrations of Halfenprox did not show mutagenic activity on both strains with and without S9 fraction. The MN assay was used to investigate the genotoxic effects of Halfenprox in human peripheral lymphocytes treated with 250, 500, 750, and 1000 μg/ml concentrations of Halfenprox for 24 and 48 h, and at 1000 μg/ml the concentration was significantly increased and the MN formation was compared with the negative control for both treatment periods. In addition, a significant decrease of the nuclear devision index (NDI) values at the higher concentrations of Halfenprox and at both treatment periods was observed.

Evaluation of Free-Radical Scavenging and Antioxidant Activity of Fruit Extracts of Three Endemic Rhamnus Species in in vitro

Abstract The antioxidant properties of Rhamnus petiolaris Boiss., R. rhodopeus Velen. subsp. anatolicus (Grub.) Browicz & Ziel., and R. thymifolius Bornm., three endemic species occurring at altitudes of 1000 – 1200 meters on volcanic rock around Afyonkarahisar was evaluated. Antiradical activity of Rhamnus petiolaris Boiss., R. thymifolius Bornm. and R. rhodopeus Velen. subsp. anatolicus (Grub.) Browicz & Ziel was found 0.47, 0.56, and 0.68 respectively. Chelation of the samples via Fe2+ ranged from 38.78 % to 54.79 %. R. rhodopeus Velen. subsp. anatolicus (Grub.) Browicz & Ziel showed the highest chelation via Fe2+, which was 54.79 %. Another method to determine the antioxidant activity of the samples was elimination of H O , which appeared to be 36.12 % in Rhamnus petiolaris Boiss., 53.89 % in R. thymifolius Bornm., and 68.732 %2 in R. rhodopeus Velen. subsp. anatolicus (Grub.) Browicz & Ziel. Also for this test, R. rhodopeus Velen. subsp. anatolicus (Grub.) Browicz & Ziel exhibited the highest antioxidant activity.