Ahmet Tamkoç

COMPARISON OF AGRONOMIC CHARACTERS, TOTAL SEED STORAGE PROTEINS AND THEIR USE FOR GENOTYPES DISCRIMINATION IN THE KENTUCKY BLUEGRASS (POA PRATENSIS L.)

ABSTRACT This research aimed to investigate the genetic diversity among 11 Turkish Kentucky bluegrass (Poa pratensis) genotypes using seed storage proteins and agronomic traits. Thirty nine polypeptide bands in range of 10 to 82 kDa were recorded. Genetic similarity among genotypes was estimated basing on Euclidian coefficients. Cluster analysis was performed using the unweighted pair-group method with arithmetic averages (UPGMA). Similarity, coefficients of 11 genotypes belonging to Poa pratensis ranged 85- 100% with low diversity of genetic variation in protein profiles. Average Euclidean distance used for agronomic data ranged from 1.51 to 8.11. This clustering pattern for protein profiles and agronomic data showed that same genotypes were revealed in same groups.

Determination of protein degradability of alfalfa hay via buffer or protease

Abstract This study was conducted to determine the effect of different vegetative periods on protein fractions of alfalfa hay and to compare two different methods for estimation of its protein degradability. In this study, 44 alfalfa hay samples cut in late vegetative, late bud, early bloom and late bloom were used. Crude protein decreased with advancing maturity (p < .05), but neutral detergent insoluble nitrogen and acid detergent insoluble nitrogen were similar. Protein degradability of the samples was estimated using Streptomyces griseus protease or borate–phosphate buffer, kinetic model, as described in Cornell Net Carbohydrate Protein System (CNCPS). Alfalfa hay samples were subjected to proteolysis for 30 h with 0.115 U/mL or 0.230 U/mL of the protease at pH 6.8. There was a moderate correlation (0.66, 0.72) between enzymatic and buffer method estimates with protein degradation. As vegetation progresses, rapidly degradable fraction (A) decreased (p < .05) and undegradable fraction (C) increased (p < .05), while potentially degradable fraction (B) was unchanged. Rumen degradable protein content decreased (p < .05) with advancing maturity. The enzyme/duration limits should be standardised and investigated whether the protease enzyme alone is sufficient for all forages.

Correlation Between Traits and Path Analysis For Seed Yield in Cephalaria syriaca Lines

This research was conducted to determined correlation among seed yield with their components and through the path coefficient analysis method, an experiment with 33 Cephalaria syriaca lines and as control, 1 population at Konya-Turkey, in 2010 and 2011. Seed yield revealed in p<0.01 level significant positive association with plant height, head number and oil yield. Also, The highest positive direct effect of oil yield on seed yield was observed followed by plant height and head number.