Ain Raal

Composition of the essential oil of Salvia officinalis L. from various European countries

Variations in the essential oil composition of Salvia officinalis L. growing in Estonia and in other European countries were determined. The oils were obtained in yields of 2.2–24.8 mL kg−1. In three samples, the content of essential oil did not conform to the EP standard (10 mL kg−1). Variations in the essential oil composition of sage were studied using capillary gas chromatographic methods. A total of 40 components were identified. The principal components in the sage oils were 1,8-cineole, camphor, α-thujone, β-thujone, borneol, and viridiflorol. The chemotypes of sage were not determined in investigated samples. The concentration of the main compounds in the drugs cultivated in Estonia varied in about the same range as the concentrations of these compounds in the oils of drugs obtained from other countries. The comparatively high concentration of toxic thujones seem to be characteristic to sage leaves cultivated in Estonia.

Essential oil composition of Pimpinella anisum L. fruits from various European countries.

Variations in the essential oil composition of Pimpinella anisum L. fruits obtained from different geographical areas of Europe were determined using capillary GC and GC–MS techniques. The essential oil content of the samples was 10.0–53.6 mL kg−1 and did not confirm to the European Pharmacopoeia standard in 5 samples out of 14. A total of 21 compounds were identified and significant quantitative differences were observed among the samples. The major component was trans-anethole (76.9–93.7%); the other principal compounds in oils were γ-himachalene (0.4–8.2%), trans-pseudoisoeugenyl 2-methylbutyrate (0.4–6.4%), p-anisaldehyde (tr-5.4%) and methylchavicol (0.5–2.3%). The highest content of trans-anethole (>90%) was found in the samples from Greece, Hungary, Scotland, Lithuania, Italy, and Germany (2 samples). Essential oil of aniseed from Estonia was rich in γ-himachalene (8.2%) and trans-pseudoisoeugenyl 2-methylbutyrate (6.4%). The sample from France contained the highest amount of anisaldehyde (5.4%) comparing with other samples (0–3.1%). β-Bourbonene and α-farnesene are determined in anise oil for the first time.

Phytochemical analysis of the essential oil of Achillea millefolium L. from various European Countries

Variations in the essential oil composition of Achillea millefolium L. growing in Estonia and in other European countries, were determined. The oils were obtained in yields of 0.9–9.5 mL kg−1. A total of 102 components were identified. The quantitatively most important components of yarrow were sabinene, β-pinene, 1,8-cineole, artemisia ketone, linalool, α-thujone, β-thujone, camphor, borneol, fenchyl acetate, bornyl acetate, (E)-β-caryophyllene, germacrene D, caryophyllene oxide, β-bisabolol, δ-cadinol, chamazulene etc. Samples from Estonia contained high amounts of monoterpenes and chamazulene. High amounts of monoterpenes and chamazulene were also found in samples from Hungary, Greek, Moldavia, Latvia, Lithuania and Germany. The oils from France, Belgium, Russia, Armenia, Spain and Italy were rich in oxygenated monoterpenes and contained a little amount of chamazulene. The drugs from Greece, Estonia, Moldavia and Scotland were rich in sesquiterpenes. The Millefolii herba grown in Estonia conforms to the European Pharmacopoeia (EP) standards in the aspect of the essential oil contents.

Content and composition of the essential oil of Chamomilla recutita (L.) Rauschert from some European countries

Variations in the essential oil composition of Chamomilla recutita (L.) Rauschert from different European countries were determined. A total of 39 components were identified, representing over 92% of the total oil yield. The principal biologically active compounds in chamomile oils were bisabolol oxide A (3.1–56.0%), α-bisabolol (0.1–44.2%), bisabolol oxide B (3.9–27.2%), cis-enyne-bicycloether (8.8–26.1%), bisabolon oxide A (0.5–24.8%), chamazulene (0.7–15.3%), spathulenol (1.7–4.8%) and (E)-β-farnesene (2.3–6.6%). In 8 chamomile samples from 13, bisabolol oxide A (27.5–56.0%) was predominant (among them in three Estonian samples). α-Bisabolol (23.9–44.2%) was predominant in the samples from Moldova, Russia and the Czech Republic. The sample from Armenia was rich in bisabolol oxide B (27.2%) and chamazulene (15.3%). The oils were obtained in yields of 0.7–6.7 mL kg−1 and the minimum limit of 4 mL kg−1 stated by the European Pharmacopoeia was exceeded only in 13 samples from 13 analysed drugs.

Polyphenolic composition of roots and petioles of Rheum rhaponticum L.

INTRODUCTION Various species of the genus Rheum (Polygonaceae) are known for their high content of medicinally important hydroxyanthraquinones. However, little information is available concerning the polyphenolic composition of garden or dietary rhubarb Rheum rhaponticum L. (R. rhaponticum). OBJECTIVE Determination of further polyphenols in the roots and petioles of R. rhaponticum. METHODOLOGY The dried plant material was extracted with 10-fold excess (v/w) of methanol and subsequently diluted five times with methanol-water (1:1) and analysed by reversed-phase liquid chromatography using tandem UV-photodiode array and mass selective detection (RP-HPLC-UV-ESI/MS(2)). Polyphenols were identified using either HPLC-ESI/MS(2) data obtained for respective commercial standards or by comparison of a parent ion fragmentation picture with the respective MS(2 )spectrum from the literature. RESULTS The roots of R. rhaponticum were very rich in various hydroxystilbenes and contained four main substance groups--derivatives of trans-piceatannol, trans-resveratrol, trans-rhapontigenin and trans-deoxyrhapontigenin. Additionally, pterostilbene acetylglucosides and a number of hydroxyanthraquinones and their glycosides were identified in the root samples. The profile of polyphenols in the petioles of R. rhaponticum was similar to that of the roots but the content of individual substances was remarkably lower. The petioles of the R. rhaponticum additionally contained significant amounts of derivatives of flavonol quercetin, which is a good antioxidant. CONCLUSION The study has shown that roots of R. rhaponticum contain a wide variety of hydroxystilbenes and deserve further consideration as a source of medicinally interesting compounds.

Phytochemical analysis of the essential oil of Thymus serpyllum L. growing wild in Estonia

Variations in the essential oil composition of Thymus serpyllum L., growing wild in Estonia (33 samples) and in some other countries (Russia, Latvia and Armenia, seven samples) were determined. The oil were obtained from Estonia (46 samples) in yields 0.6–4.4 and 1.9–8.2 mL kg–1 in other countries. The T. serpyllum herb grown in Estonia usually did not confirmed to the EP standard in the aspect of the essential oil contents (3.0 mL kg–1). Variations in the essential oil composition of wild thyme were studied using capillary gas chromatographic methods. A total of 94 components were identified. Thymol and carvacrol, mentioned in literature as principial components, are not the main components of the essential oil of wild thyme growing in Estonia. (E)-nerolidol, caryophyllene oxide, myrcene and borneol chemotypes of wild thyme drug are distinguishable. The chemical composition of samples from Russia, Latvia and Armenia is very variable.

A survey of Estonian consumer expectations from the pharmacy service and a comparison with the opinions of pharmacists

ObjectiveTo assess the preferences of pharmacy customers when choosing a pharmacy and their expectations of the service, and␣comparing these with the opinions of pharmacists.Main outcome measureOpinion and satisfaction of community pharmacy clients in Estonia.MethodsA written survey was carried out among pharmacy customers (n=1979) in cities (in 3 community pharmacies), towns (in 2 community pharmacies), and in small towns (in 2 community pharmacies). The survey was also carried out among community pharmacists (n=135) in different regions of Estonia.ResultsWhen choosing a pharmacy, its location was considered most important, costs and wide choice are less important. The most important expectations of customers’ included help choosing the right medicine, as well as professional consultation. Preferences and expectations of pharmacy customers depended on their age, gender and income. Parking space, quickness and pleasantness were considered important by men. Costs and wide choice were considered to be more important by women. Pharmacists wish to give patients more information, but they overestimate the importance of quick service. Customers favoured privacy, discretion and confidentiality more. These characteristics were especially important to younger well-paid people living in big cities.ConclusionIn contrast to the opinions offered by pharmacists’, a rapid customer service is so not as important as the quality of service in pharmacy according to customers. They also emphasised that pharmacies should highlight the choice of products, quality of service, a professional consultation, as well as ensure privacy.

Trans-Resveratrol Alone and Hydroxystilbenes of Rhubarb (Rheum rhaponticum L.) Root Reduce Liver Damage Induced by Chronic Ethanol Administration : a Comparative Study in Mice

The hepatoprotective effects and pharmacokinetics of trans‐resveratrol and hydroxystilbenes of the garden rhubarb (Rheum rhaponticum L., R. rhaponticum) root ethanol extract were studied.

Composition of the Essential Oil of Levisticum Officinale W.D.J. Koch from Some European Countries

Abstract Variations in the essential oil composition of Leviticum officinale W.D.J. Koch from different European countries were determined using capillary GC and GC/MS methods. The oils were obtained in yields of 0.11–1.80% from dried cut roots and 0.09% from leaves. A total of 48 components were identified, representing over 87% of the total yield of oil. Ten compounds not earlier reported: trans-p-mentha-2,8-dien-1-ol, iso-thujyl alcohol, p-mentha-1,5-dien-8-ol, bicyclo[3.2.0]heptan-3-ol, 2-methylene-6,6-dimethyl, trans-carveol, perillaldehyde, sabinyl acetate, perillyl alcohol, methyl ester of methylpentadecate acid, and methyl hexadecadienate acid. The principal components in the oils of L. officinale roots were β-phellandrene (0.1–48.9%), pentylcyclohexadiene (0–12.3%), trans-sabinyl acetate (0–12.1%), α-terpinyl acetate (0–26.1%), (Z)-3-butylidene phthalide (0.1–31.2%), and (Z)-ligustilide (0.2–70.9%). Phthalide isomers were predominant (73.2–82.6%) in the oils from Estonia, France, and Belgium. The roots oil of L. officinale from Scotland was rich in β-phellandrene (48.9%) and phenylacetaldehyde (17.2%). Maximum content of trans-sabinyl and α-terpinyl acetates (total 38.2%) was found in the oil from Holland. Estonian L. officinale root oil contained in high quantities (E)-ligustilide (52.4–70.9%) and pentylcyclohexadiene (12.3%). The L. officinale leaf oil cultivated in Estonia contained in high amounts α-terpinyl acetate (55.8%) and β-phellandrene (11.3%). The content of (Z)-ligustilide (17.0%) in the leaf oil was smaller compared with the root oil.

Complementary Treatment of the Common Cold and Flu with Medicinal Plants – Results from Two Samples of Pharmacy Customers in Estonia

The aim of the current survey was to investigate the complementary self-treatment of the common cold and flu with medicinal plants among pharmacy customers in Estonia. A multiple-choice questionnaire listing 10 plants and posing questions on the perceived characteristics of cold and flu, the effectiveness of plants, help-seeking behaviour, self-treatment and sources of information, was distributed to a sample of participants in two medium size pharmacies. The participants were pharmacy customers: 150 in Tallinn (mostly Russian speaking) and 150 in Kuressaare (mostly Estonian speaking). The mean number of plants used by participants was 4.1. Of the respondents, 69% self-treated the common cold and flu and 28% consulted with a general practitioner. In general, medicinal plants were considered effective in the treatment of the above-mentioned illnesses and 56% of the respondents had used exclusively medicinal plants or their combination with OTC medicines and other means of folk medicine for treatment. The use of medicinal plants increased with age and was more frequent among female than male respondents. Among Estonian-speaking customers lime flowers, blackcurrant and camomile were more frequently used, and among Russian speaking customers raspberry and lemon fruits. Regardless of some statistically significant differences in preferred species among different age, education, sex and nationality groups, the general attitude towards medicinal plants for self-treatment of the common cold and flu in Estonia was very favourable.