Aisuo Wang
Charles Sturt University
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Publication
Featured researches published by Aisuo Wang.
Genome Announcements | 2013
Aisuo Wang; Julie Pattemore; Gavin Ash; Angela H. Williams; James K. Hane
ABSTRACT Bacillus thuringiensis has been widely used as a biopesticide for a long time. Its molluscicidal activity, however, is rarely realized. Here, we report the genome sequence of B. thuringiensis strain DAR 81934, a strain with molluscicidal activity against the pest snail Cernuella virgata.
Scientific Reports | 2015
Aisuo Wang; Gavin Ash
Fifty complete Bacillus genome sequences and associated plasmids were compared using the “feature frequency profile” (FFP) method. The resulting whole-genome phylogeny supports the placement of three Bacillus species (B. thuringiensis, B. anthracis and B. cereus) as a single clade. The monophyletic status of B. anthracis was strongly supported by the analysis. FFP proved to be more effective in inferring the phylogeny of Bacillus than methods based on single gene sequences [16s rRNA gene, GryB (gyrase subunit B) and AroE (shikimate-5-dehydrogenase)] analyses. The findings of FFP analysis were verified using kSNP v2 (alignment-free sequence analysis method) and Harvest suite (core genome sequence alignment method).
PLOS ONE | 2017
Aisuo Wang; David Gopurenko; Hanwen Wu; Brendan J. Lepschi
Invasive grass weeds reduce farm productivity, threaten biodiversity, and increase weed control costs. Identification of invasive grasses from native grasses has generally relied on the morphological examination of grass floral material. DNA barcoding may provide an alternative means to identify co-occurring native and invasive grasses, particularly during early growth stages when floral characters are unavailable for analysis. However, there are no universal loci available for grass barcoding. We herein evaluated the utility of six candidate loci (atpF intron, matK, ndhK-ndhC, psbE—petL, ETS and ITS) for barcode identification of several economically important invasive grass species frequently found among native grasses in eastern Australia. We evaluated these loci in 66 specimens representing five invasive grass species (Chloris gayana, Eragrostis curvula, Hyparrhenia hirta, Nassella neesiana, Nassella trichotoma) and seven native grass species. Our results indicated that, while no single locus can be universally used as a DNA barcode for distinguishing the grass species examined in this study, two plastid loci (atpF and matK) showed good distinguishing power to separate most of the taxa examined, and could be used as a dual locus to distinguish several of the invasive from the native species. Low PCR success rates were evidenced among two nuclear loci (ETS and ITS), and few species were amplified at these loci, however ETS was able to genetically distinguish the two important invasive Nassella species. Multiple loci analyses also suggested that ETS played a crucial role in allowing identification of the two Nassella species in the multiple loci combinations.
Mitochondrial DNA Part B | 2017
Aisuo Wang; Hanwen Wu; David Gopurenko
Abstract Nassella neesiana (Chilean needle grass) is a serious weed in Australia, and has been included in the list of Weeds of National Significance (WoNS). We present here, the complete chloroplast sequence of N. neesiana reconstructed from Illumina whole genome sequencing. The complete chloroplast sequence is 137,700 bp in size, and has similar gene content and structure as other published chloroplast genomes of Stipeae. The N. neesiana chloroplast genome is deposited at GenBank under accession number MF480752.
Journal of Phylogenetics & Evolutionary Biology | 2015
Aisuo Wang; Gavin Ash; M. Hodda; Farzad Jahromi
Pest snails are economically important pests of the grain industry. Nematode based bioagent appears to be a hope for controlling pest snails in an environment friendly way. Based on the dataset of 18S rRNA gene sequences, we propose a molecular phylogeny of nematodes baited with Cernuella virgata in soils collected from southern states of Australia. A total of 12 species (representing eight genera of nematodes) were identified and the inferred phylogenetic trees (Neighbor-Joining and Minium Evolution) placed them within three (I, IV and VII) out of the seven clades, indicating the possibility of multiple origins of snail parasitism. In Clade I and Clade VII, nematodes associated with Cernuella virgata formed sister group relationships with some slug – parasitic nematodes. We assume that snail – parasitic nematodes and slug - parasitic nematodes might share common ancestors in their evolutionary histories.
Plant and Soil | 2012
Yj Guo; Y. Ni; Harsh Raman; Bree A. L. Wilson; Gavin Ash; Aisuo Wang; Guangdi Li
Crime in Australia: International Connections | 2004
Paul Roffey; Pam Provan; Michelle Duffy; Aisuo Wang; Christopher Blanchard; Lyndall Angel
19th Australasian Weeds Conference (2014) | 2014
Aisuo Wang; David Gopurenko; Hanwen Wu; Rex Stanton; Brendan J. Lepschi
20th Australasian Weeds Conference (2016) | 2016
Aisuo Wang; Hanwen Wu; David Gopurenko
Molluscan Research | 2006
Aisuo Wang; Paul Roffey; Christopher Blanchard; Lyndall Angel; Pamela Provan
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