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Featured researches published by Akatsuki Kawakoshi.


Zoological Science | 2001

CNP is the only Natriuretic Peptide in an Elasmobranch Fish, Triakis scyllia

Akatsuki Kawakoshi; Susumu Hyodo; Yoshio Takei

Abstract The natriuretic peptide (NP) family consists of endocrine ANP/BNP/VNP in the heart and paracrine CNP in the brain in vertebrates ranging from teleosts to mammals. In elasmobranchs, however, only CNP has been identified thus far in the heart and brain. To delineate the molecular evolution of this hormone family, it is essential to determine whether CNP is the only NP in this primitive fish group. In the present study, PCR cloning of all types of piscine NP was performed from the heart and brain of a dogfish, Triakis scyllia, using degenerate primers that amplified eel ANP, VNP and CNP. However, only CNP cDNA with an identical sequence was cloned from the heart and brain. Southern blot analysis showed that the CNP gene is a single copy gene, showing that endocrine CNP from the heart and paracrine CNP in the brain originate from the same gene. Since expression of the CNP gene was so high as demonstrated by Northern blot analysis, the abundantly expressed CNP mRNA could have interfered the amplification of other NP mRNAs expressed in small amounts. Therefore, a method was developed to cleave the cloned CNP mRNA specifically at the 3′-untranslated region with RNase H. After removal of the cloned CNP mRNA by this technique, no other NP cDNAs could be cloned, but small amounts of CNP cDNAs with shorter 3′ sequence were amplified. These results strongly suggest that only CNP is present in elasmobranchs. Thus, it is likely that CNP is an ancestral form of the NP family and endocrine ANP/BNP/VNP have appeared later in the vertebrate evolution.


Journal of Molecular Endocrinology | 2004

Four natriuretic peptides (ANP, BNP, VNP and CNP) coexist in the sturgeon: identification of BNP in fish lineage

Akatsuki Kawakoshi; Susumu Hyodo; Koji Inoue; Y Kobayashi; Yoshio Takei


Journal of Molecular Endocrinology | 2003

A single and novel natriuretic peptide is expressed in the heart and brain of the most primitive vertebrate, the hagfish (Eptatretus burgeri)

Akatsuki Kawakoshi; Susumu Hyodo; Akikazu Yasuda; Yoshio Takei


General and Comparative Endocrinology | 2007

Identification of a ghrelin-like peptide in two species of shark, Sphyrna lewini and Carcharhinus melanopterus.

Akatsuki Kawakoshi; Hiroyuki Kaiya; Larry G. Riley; Tetsuya Hirano; E. Gordon Grau; Mikiya Miyazato; Hiroshi Hosoda; Kenji Kangawa


Journal of Experimental Zoology Part A: Comparative Experimental Biology | 2006

Contribution of comparative fish studies to general endocrinology: Structure and function of some osmoregulatory hormones

Yoshio Takei; Akatsuki Kawakoshi; Takehiro Tsukada; Shinya Yuge; Maho Ogoshi; Koji Inoue; Susumu Hyodo; Hideo Bannai; Satoru Miyano


General and Comparative Endocrinology | 2006

Identification of a natriuretic peptide (NP) in cyclostomes (lamprey and hagfish) : CNP-4 is the ancestral gene of the NP family

Akatsuki Kawakoshi; Susumu Hyodo; Masumi Nozaki; Yoshio Takei


General and Comparative Endocrinology | 2006

Multiple natriuretic peptides coexist in the most primitive extant ray-finned fish, bichir Polypterus endlicheri

Albert Ventura; Akatsuki Kawakoshi; Koji Inoue; Yoshio Takei


General and Comparative Endocrinology | 2006

Extremely high conservation in the untranslated region as well as the coding region of CNP mRNAs throughout elasmobranch species

Susumu Hyodo; Akatsuki Kawakoshi; Ray C. Bartolo; Yoshio Takei; Tes Toop; John A. Donald


Zoological Science | 2005

MULTIPLE NATRIURETIC PEPTIDES IDENTIFIED IN THE MOST PRIMITIVE EXTANT RAY-FINNED FISH CREATE A DEEPER UNDERSTANDING OF THEIR EVOLUTION(Physiology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)

Albert Ventura; Akatsuki Kawakoshi; Koji Inoue; Yoshio Takei


Proceedings of the Japan Society for Comparative Endocrinology | 2005

IDENTIFICATION OF GHRELIN IN TWO SPECIES OF SHARK, SPHYRNA LEWINI AND CARCHARHINUS MELANOPTERUS

Akatsuki Kawakoshi; Hiroyuki Kaiya; Larry G. Riley; Tetsuya Hirano; Gordon Grau; Kenji Kangawa

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