Akihiro Moriwaki
Shimane University
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Featured researches published by Akihiro Moriwaki.
Fems Microbiology Letters | 2008
Junichi Kihara; Akihiro Moriwaki; Nozomi Tanaka; Chihiro Tanaka; Makoto Ueno; Sakae Arase
We isolated and characterized Bipolaris melanin regulation 1 gene (BMR1) encoding a transcription factor for melanin biosynthesis genes in the phytopathogenic fungus Bipolaris oryzae. Sequence analysis showed that the BMR1 gene encodes a putative protein of 1012 amino acids that has 99% sequence similarity to transcription factor Cmr1 of Cochliobolus heterostrophus. The predicted B. oryzae Bmr1 protein has two DNA-binding motifs, two Cys2His2 zinc finger domains, and a Zn(II)2Cys6 binuclear cluster domain at the N-terminal region of Bmr1. Targeted disruption of the BMR1 gene showed that BMR1 is essential for melanin biosynthesis in B. oryzae. The overexpression of the BMR1 gene led to more dark colonies than in the wild-type strain under dark conditions. Real-time PCR analysis showed that the BMR1 expression of the overexpression transformant was about 10-fold that of the wild type under dark conditions and of the expression of three melanin biosynthesis genes. These results indicated that BMR1 encodes the transcription factor of melanin biosynthesis genes in B. oryzae.
Current Microbiology | 2008
Akihiro Moriwaki; Hirofumi Katsube; Makoto Ueno; Sakae Arase; Junichi Kihara
Bipolaris oryzae is a filamentous ascomycetous fungus that causes brown leaf spot disease in rice. We isolated and characterized BLR2, a gene that encodes a putative blue-light regulator similar to Neurospora crassa white collar-2 (WC-2). The deduced amino acid sequence of the BLR2 showed significant homology to other fungal blue-light regulator proteins in the Per-Arnt-Sim (PAS) protein–protein interaction domain, nuclear localization signal, and GATA zinc finger DNA-binding domains. The BLR2-silenced transformants hardly produced conidia in the subsequent dark condition after near-ultraviolet (NUV) irradiation. Furthermore, the BLR2-silenced transformants suppressed the photolyase (PHR1) gene expression enhanced by NUV irradiation. These results indicate that BLR2 is necessary not only for conidial formation, but also for NUV radiation-enhanced photolyase gene expression in B. oryzae.
Fems Microbiology Letters | 2006
Akihiro Moriwaki; Emiko Kubo; Sakae Arase; Junichi Kihara
Fems Microbiology Letters | 2004
Akihiro Moriwaki; Junichi Kihara; Tsutomu Kobayashi; Toshiko Tokunaga; Sakae Arase; Yuichi Honda
Fems Microbiology Letters | 2007
Akihiro Moriwaki; Makoto Ueno; Sakae Arase; Junichi Kihara
Current Genetics | 2004
Junichi Kihara; Akihiro Moriwaki; Makoto Ueno; Toshiko Tokunaga; Sakae Arase; Yuichi Honda
Microbiological Research | 2007
Akihiro Moriwaki; Junichi Kihara; Chie Mori; Sakae Arase
Pigment Cell Research | 2004
Junichi Kihara; Akihiro Moriwaki; Machiko Ito; Sakae Arase; Yuichi Honda
Fems Microbiology Letters | 2007
Junichi Kihara; Akihiro Moriwaki; Nozomi Tanaka; Makoto Ueno; Sakae Arase
Current Genetics | 2004
Junichi Kihara; Akihiro Moriwaki; Nobuhito Matsuo; Sakae Arase; Yuichi Honda