Akira Shibue

Electrochemical luminescence-based homogeneous immunosensor

Abstract Luminol capable of generating electrochemical luminescence was employed as a label of immunoassay. A luminol-labeled antibody generated luminescence upon anodic oxidation and the luminescence decreased markedly in the presence of antigen. The decrease was correlated to the antigen concentration as low as 10 −11 g/ml antigen. The proposed method was operated in an optoelectrochemical cell. A miniaturized and sensitive immunosensing can be developed by fabricating a small cell with a very thin layer.