Akw Lie

Glucose-6-phosphate dehydrogenase deficiency and hematopoietic stem cell transplantation

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked hemolytic enzymopathy affecting 3% of Southern Chinese males. Among 275 adult allogeneic hematopoietic stem cell transplantations (SCT), five cases (1.8%) each of donors and recipients were G6PD deficient. Among 107 autologous SCT, four patients (3.7%) were G6PD deficient. All subjects were male, except for two female patients with chronic myeloid leukemia (CML). The incidence of G6PD deficiency in female CML patients was significantly higher than the background female incidence (P = 0.004), but comparable with that in the males (P = 0.664). There was no significant hemolysis or delay in red cell engraftment, and all but one patient converted to donor G6PD screening status. One female patient achieved partial correction of her G6PD status and relapsed at 10 months. We suggest that G6PD deficiency should be tested for in all marrow donors and recipients in susceptible populations. From our data, there is a suggestion of increased clinical incidence of G6PD deficiency in female patients with multi-lineage clonal marrow disorders.Bone Marrow Transplantation (2002) 29, 399–402. doi:10.1038/sj.bmt.1703369

Proteomic identification of stress-response heat shock protein markers associated with tumor recurrence in patients with hepatocellular carcinoma

12.2%) largely present in the lobules. Although both of them were phenotypically mature, and strong T cell stimulators as shown by T cell cytokine (ELISA) and division (CFSE dilution), CD11c DCs induced potent T cell response while B220DC induced T cell hyporesponsiveness. Administration of CD11cDCs accelerated cardiac allograft rejection, while B220DCs significantly prolonged graft survival. This hyporesponsiveness is not due to inhibition of DC-T cell interaction, but rather through an active process of stimulating T cell apoptosis. Compared to B220 DCs that expressed mRNA of TLR 1, 2, 6, 7 and 9, CD11cDCs expressed all TLR 1 to 9, implicating that CD11c DCs may more extensively involve in innate immunity. CpG (TLR9 ligation) stimulated very high IL-12 in CD11c DCs, but high IL-10 (not IL-12) in B220 DCs. These data help to better understand the complex of liver immune regulation in chronic liver diseases and transplantation.

A comparison of non-TBI and TBI conditioning regimens in bone marrow recipients undergoing match-unrelated transplantation in Hong Kong

Combined annual meetings of CIBMTR and the American Society of Blood and Marrow Transplantation (ASBMT)