Alan Conneely

Nanosecond laser silicon micromachining

We present theoretical calculations and experimental measurements of silicon micromachining rates, efficiency of laser pulse utilization, and morphology changes under UV nanosecond pulses with intensities ranging from 0.5 GW/cm2 to 150 GW/cm2. Three distinct irradiance regimes are identified based on laser intensity. At low intensity, proper gas dynamics and ablation vapor plume kinetics are taken into account in our theoretical modeling. At medium high intensity, we incorporate the proper plasma dynamics, and predict the effects of the laser generated vapor plasma and the electron hole plasma on the laser-matter interaction. At even higher intensity, we attribute the observed increased ablation rate to energy re-radiation from the laser heated hot plasma, the strong shock wave, and the accompanied strong shock wave heating effects. Experimentally measured data in these regimes agree well with our calculations, without changing parameters in the calculations used for the three regimes. Our results can be applied toward quantitatively characterize the behavior of ablation results under different laser parameters to achieve optimal results for micromachining of slots and vias on silicon wafers.

Blue-Light Inhibition of Listeria monocytogenes Growth Is Mediated by Reactive Oxygen Species and Is Influenced by σB and the Blue-Light Sensor Lmo0799.

ABSTRACT Listeria monocytogenes senses blue light via the flavin mononucleotide-containing sensory protein Lmo0799, leading to activation of the general stress response sigma factor SigB (σB). In this study, we investigated the physiological response of this foodborne pathogen to blue light. We show that blue light (460 to 470 nm) doses of 1.5 to 2 mW cm−2 cause inhibition of growth on agar-based and liquid culture media. The inhibitory effects are dependent on cell density, with reduced effects evident when high cell numbers are present. The addition of 20 mM dimethylthiourea, a scavenger of reactive oxygen species, or catalase to the medium reverses the inhibitory effects of blue light, suggesting that growth inhibition is mediated by the formation of reactive oxygen species. A mutant strain lacking σB (ΔsigB) was found to be less inhibited by blue light than the wild type, likely indicating the energetic cost of deploying the general stress response. When a lethal dose of light (8 mW cm−2) was applied to cells, the ΔsigB mutant displayed a marked increase in sensitivity to light compared to the wild type. To investigate the role of the blue-light sensor Lmo0799, mutants were constructed that either had a deletion of the gene (Δlmo0799) or alteration in a conserved cysteine residue at position 56, which is predicted to play a pivotal role in the photocycle of the protein (lmo0799 C56A). Both mutants displayed phenotypes similar to the ΔsigB mutant in the presence of blue light, providing genetic evidence that residue 56 is critical for light sensing in L. monocytogenes. Taken together, these results demonstrate that L. monocytogenes is inhibited by blue light in a manner that depends on reactive oxygen species, and they demonstrate clear light-dependent phenotypes associated with σB and the blue-light sensor Lmo0799. IMPORTANCE Listeria monocytogenes is a bacterial foodborne pathogen that can cause life-threatening infections in humans. It is known to be able to sense and respond to visible light. In this study, we examine the effects of blue light on the growth and survival of this pathogen. We show that growth can be inhibited at comparatively low doses of blue light, and that at higher doses, L. monocytogenes cells are killed. We present evidence suggesting that blue light inhibits this organism by causing the production of reactive oxygen species, such as hydrogen peroxide. We help clarify the mechanism of light sensing by constructing a “blind” version of the blue-light sensor protein. Finally, we show that activation of the general stress response by light has a negative effect on growth, probably because cellular resources are diverted into protective mechanisms rather than growth.

Laser soldering and inspection of fine pitch electronic components

Abstract The increasing miniaturisation of integrated circuits has resulted in devices with lead spacings as small as 0.008″. Laser soldering has the potential to overcome many of the problems encountered with these devices by conventional soldering technologies. Nd:YAG laser soldering of a 224-lead ceramic chip onto a FR4 circuit board is described, using experimental design to optimise the laser parameters for soldering. In order to improve solder joint quality and repeatability, a rigorous thermal analysis is undertaken using a finite element model to investigate the temperature rise variations between the joints. Thermocouple measurements are made in real time of the laser soldering process to confirm the finite element model predictions.

Investigation of a method for the determination of the focused spot size of industrial laser beams based on the drilling of holes in Mylar film

The focussed spot size of industrial laser beams is a critical processing parameter in most laser machining applications as it determines the machined feature size and the irradiance produced by the laser at the material interface. There are a number of standard methods available for accurately measuring and analysing the focussed spot. These methods often require expensive equipment that can be time consuming and difficult to set up in a production environment. This paper presents an investigation into a cost effective and straightforward method for the measurement of focussed laser spot sizes based on drilling of holes in mylar film. It can be shown that the slope of a plot of the square of the hole diameter versus the natural log of the laser pulse energy is equal to twice the square of the spot radius. A measure of the laser spot size can be calculated by generating laser-drilled holes at number of laser pulse energies. The practicality and accuracy of this method is investigated in this paper for a number of laser types including a diode pumped solid state laser (UV DPSS) operating at the third harmonic (355nm), a femtosecond laser and a flash lamp pumped Nd:YAG laser. A comparison between the measured results and the results generated with other available techniques is also presented.

Visible and UVA light as a potential means of preventing Escherichia coli biofilm formation in urine and on materials used in urethral catheters

Catheter-associated urinary tract infections are the most common hospital-acquired infection, for which Escherichia coli is the leading cause. This study investigated the efficacy of 385nm and 420nm light for inactivation of E. coli attached to the silicone matrix of a urinary catheter. Using urine mucin media, inactivation of planktonic bacteria and biofilm formation was monitored using silicone coupons. Continuous irradiance with both 385nm and 420nm wavelengths with starting cell density population 103CFU ml-1 reduced planktonic suspensions of E. coli to below the detection level after 2h and 6h, respectively. Bacterial attachment to silicone was successfully prevented during the same treatment. Inactivation by 385nm and 420nm was found to be dependent on media, cell density and oxygen, with less inhibition on planktonic suspensions when higher starting cell densities were used. In contrast to planktonic suspensions in PBS, continuous irradiance of pre-established biofilms showed a greater reduction in survival compared to urine mucin media after 24h. Enhanced inhibition for 385nm and 420nm light in urine mucin media was associated with increased production of reactive oxygen species. These findings suggest 385nm and 420nm light as a promising antimicrobial technology for the prevention of biofilm formation on urethral catheters.

Combined anodizing and picosecond laser treatment to control the corrosion rate of biodegradable magnesium alloy AZ31

The corrosion rate of magnesium alloys is generally too high for biodegradable implant applications. This work explored combinations of anodizing and picosecond laser surface treatments to modify the corrosion response of magnesium alloy AZ31. Anodizing of the AZ31 in NaOH solutions produced porous oxide layer structures. Shallow laser treatment of these anodized surfaces, using low pulse powers, resulted mainly in oxide ablation and impaired corrosion resistance. Higher pulse power, resulting in rapid melting and resolidification into the substrate, provided an improved corrosion response. The refined grain structure produced is approximately only 5 µm deep and therefore has minimal influence on bulk mechanical properties. It is therefore a suitable process for surface modifications on small medical device structures. Controlling the initial point of degradation has been demonstrated by the use of selective laser treatment of the AZ31 surface.

Analysis of debris generated during UV laser micromachining of silicon

The generation of debris is critical in the future application of laser technology in IC, MEMS, MOEMS manufacture. Re-deposition of debris is also critical in optimising throughput of multi-pass laser ablative processes. In this study, the debris formed in laser micromachining of wafer grade silicon is investigated. Details of the laser workstation, based on a UV DPSS laser, will be presented and the development of real time diagnostic capabilities and off-line techniques will then be described. A real time imaging capability has been used to monitor plasma and shock front propagation with nanosecond resolution. The detection system is also used to monitor spectral emission of debris and micron-sized particulate ejected from the silicon surface. Emission spectroscopy of the laser ablated silicon in the plasma show spectral features that are characteristic of atomic and molecular species on timescales of nanoseconds and microseconds, respectively, after the laser pulse. Off-line characterisation techniques have focused on investigating the distribution and chemical composition of entrapped particulate. A number of novel experimental configurations for particulate entrapment, both adjacent to and remote from the laser-ablated surface, will be described. EDX results indicate that debris generated in air is composed principally of oxygen and silicon. Additional SEM results indicate that the particulate size grows through aggregation and depends on the environment in which they are generated.

Development of a soldering/inspection workcell for surface mount devices

The small focused spot size and localized heating possible with laser soldering makes it an attractive alternative technique for bonding surface mount devices with small lead pitch onto a printed wiring board. Using a Nd:YAG laser, we have applied this technique to reflow soldering of a test device-a 224-pin quad ceramic chip (lead spacing 25 mil)-onto pretinned substrates. The soldering step was incorporated into a larger workcell, in which an Adept robot was used to place the component in position and also to scan the laser beam, delivered via a fiber, over the leads to be soldered. The mobile laser head was modified to accept a miniature CCD camera coupled to a Cognex vision system, which allowed coincident viewing of the soldering process and postsoldering inspection of the joints.

Solar irradiance limits the long-term survival of Listeria monocytogenes in seawater

Seafood has often been implicated in outbreaks of food‐borne illness caused by Listeria monocytogenes but the source of contamination is usually not known. In this study we investigated the possibility that this pathogen could survive in seawater for an extended time period. Freshly collected seawater samples were inoculated with 1 × 108 CFU per ml of L. monocytogenes EGD‐e and survival was monitored by plate counting for up to 25 days. When incubated in the dark, either at ambient temperatures (4–14°C) or at 16°C, >104 CFU per ml survivors were present after 25 days. However, when the seawater cell suspensions were exposed to ambient light (solar irradiation) and temperatures, L. monocytogenes lost viability rapidly and no survivors could be detected after the 80 h time point. Both UV‐A and visible light in the blue region of the spectrum (470 nm) were found to contribute to this effect. The stress inducible sigma factor σB was found to play a role in survival of L. monocytogenes in seawater. Together these data demonstrate that solar irradiation is a critical determinant of L. monocytogenes survival in marine environments. The data further suggest the possibility of controlling this food‐borne pathogen in food‐processing environments using visible light.

Exploring the Potential of Light to Prevent and Treat Microbial Biofilms in Medical and Food Applications

Biofilms are complex communities of microbial cells covered in an exopolysaccharide matrix and adhered to a surface. Colonization of medical devices is a significant problem in healthcare-associated infections, especially those related to implanted medical devices such as intravascular catheters and urinary catheters. Recent advances in light technology highlight the potential for light inhibition of biofilm formation in medical devices. This chapter reviews the microbial responses to light, mechanisms of photoinactivation, and some recent research on the use of light to eliminate biofilms. Although light holds a tremendous opportunity to treat antibiotic-resistant infections, challenges in relation to patient safety need to be evaluated. We also discuss some of the research aimed at translating the knowledge into clinical treatment of biofilm-associated infections.